Abstract
Acupuncture was proven beneficial in treating allergic inflammation. We aimed to explore the regulation underlying the effects of acupuncture on Feishu, an acupoint most commonly used in the acupuncture therapy for respiratory diseases, with respect to the system of sympathetic nerve neurotransmitter acetylcholine (Ach). Male Wistar rats were randomly grouping. No treatment was taken in the normal group. Allergic asthma was induced using ovalbumin on the model, Feishu acupuncture, and sham acupuncture groups; then control or acupuncture treatment lasting for 3 weeks was performed. Bronchoalveolar lavage fluid (BALF) from the four groups was examined. And pulmonary tissues were subjected to histological analysis with H&E staining; besides, immunofluorescent staining, quantitative PCR, and western blot were used to detect synthetase (ChAT) and Ach hydrolase (AchE), and its muscarinic receptors (mAchRs) M1-M3. There was inflammatory infiltration in the lung upon allergic asthma, which was alleviated by the Feishu acupuncture. The eosinophilic granulocytes, neutrophils, and lymphocytes in BALF from the Feishu acupuncture group were all significantly decreased compared with those of the model and sham acupuncture groups. The specific acupuncture on Feishu upon allergic asthma put down the pulmonary expression of ChAT, repaired at the level of gene expression the pulmonary expression of mAchR M1, and restored the pulmonary expression of mAchR M2 (especially in the bronchiolar epithelium) which has a role in inhibiting Ach release; while sham acupuncture had no effect. These results confirmed the therapeutic effects of Feishu acupuncture on allergic asthma, suggesting that the mechanisms may involve suppression of the Ach signal both from its synthesis and during its release.
Similar content being viewed by others
References
McDonald, J.L., P.K. Smith, C.A. Smith, C. Changli Xue, B. Golianu, A.W. Cripps, and G. Mucosal Immunology Research. 2016. Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis. Annals of Allergy, Asthma, and Immunology 116: 497–505. https://doi.org/10.1016/j.anai.2016.04.002.
Cheng, X.N. 2016. Chinese acupuncture and moxibustion. China: Foreign Language Press.
Chen, H.Y., S.G. Li, W.C. Cho, and Z.J. Zhang. 2013. The role of acupoint stimulation as an adjunct therapy for lung cancer: a systematic review and meta-analysis. BMC Complementary and Alternative Medicine 13: 362. https://doi.org/10.1186/1472-6882-13-362.
Tian, Y., Y. Li, J. Li, Y. Xie, M. Wang, Y. Dong, L. Li, J. Mao, L. Wang, and S. Luo. 2015. Bufei Yishen granule combined with acupoint sticking improves pulmonary function and morphormetry in chronic obstructive pulmonary disease rats. BMC Complementary and Alternative Medicine 15: 266. https://doi.org/10.1186/s12906-015-0787-0.
Coulson, F.R., and A.D. Fryer. 2003. Muscarinic acetylcholine receptors and airway diseases. Pharmacology & Therapeutics 98: 59–69.
Soler, X., and J. Ramsdell. 2014. Anticholinergics/antimuscarinic drugs in asthma. Current Allergy and Asthma Reports 14: 484. https://doi.org/10.1007/s11882-014-0484-y.
Kistemaker, L.E., and R. Gosens. 2015. Acetylcholine beyond bronchoconstriction: roles in inflammation and remodeling. Trends in Pharmacological Sciences 36: 164–171. https://doi.org/10.1016/j.tips.2014.11.005.
Bell, L.A., K.A. Bell, and A.R. McQuiston. 2015. Acetylcholine release in mouse hippocampal CA1 preferentially activates inhibitory-selective interneurons via alpha4beta2* nicotinic receptor activation. Frontiers in Cellular Neuroscience 9: 115. https://doi.org/10.3389/fncel.2015.00115.
Pera, T., and R.B. Penn. 2014. Crosstalk between beta-2-adrenoceptor and muscarinic acetylcholine receptors in the airway. Current Opinion in Pharmacology 16: 72–81. https://doi.org/10.1016/j.coph.2014.03.005.
Ikeda, T., A.S. Anisuzzaman, H. Yoshiki, M. Sasaki, T. Koshiji, J. Uwada, A. Nishimune, H. Itoh, and I. Muramatsu. 2012. Regional quantification of muscarinic acetylcholine receptors and beta-adrenoceptors in human airways. British Journal of Pharmacology 166: 1804–1814. https://doi.org/10.1111/j.1476-5381.2012.01881.x.
Gao, X., Y. Zhao, Y. Su, K. Liu, X. Yu, C. Cui, Z. Yang, H. Shi, X. Jing, and B. Zhu. 2016. beta1/2 or M2/3 receptors are required for different gastrointestinal motility responses induced by acupuncture at heterotopic or homotopic acupoints. PLoS One 11: e0168200. https://doi.org/10.1371/journal.pone.0168200.
Chen, S.P., Y. Kan, J.L. Zhang, J.Y. Wang, Y.H. Gao, L.N. Qiao, X.M. Feng, Y.X. Yan, and J.L. Liu. 2016. Involvement of hippocampal acetylcholinergic receptors in electroacupuncture analgesia in neuropathic pain rats. Behavioral and Brain Functions 12: 13. https://doi.org/10.1186/s12993-016-0096-x.
Liu, Y., B. Zhang, S. Zhang, J. Qi, Z. Zhang, L. Liu, and X. Fang. 2010. Nerve growth factor mediated SH2-Bbeta/Akt signal pathway activated in allergic airway challenge in mice. Respirology 15: 80–87. https://doi.org/10.1111/j.1440-1843.2009.01648.x.
Shoseyov, D., H. Bibi, S. Offer, O. Schwob, M. Krimsky, M. Kleiman, and S. Yedgar. 2005. Treatment of ovalbumin-induced experimental allergic bronchitis in rats by inhaled inhibitor of secretory phospholipase a(2). Thorax 60: 747–753. https://doi.org/10.1136/thx.2005.043695.
Fang, C., X. Li, H. Liang, L. Xue, L. Liu, C. Yang, G. Gao, and X. Jiang. 2015. Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation. International Journal of Clinical and Experimental Pathology 8: 12053–12063.
Guo, Y., and J.Q. Fang. 2012. Experimental acupuncture. Beijing: China Press of Traditional Chinese Medicine.
Liu, H.T., Y.L. Liu, S.T. Song, and T.M. Ma. 2015. Methods for accurately locating “Feishu” (BL 13) in rats. Zhen Ci Yan Jiu 40: 329–330.
McDonald, J.L., A.W. Cripps, and P.K. Smith. 2015. Mediators, receptors, and Signalling pathways in the anti-inflammatory and antihyperalgesic effects of acupuncture. Evidence-based Complementary and Alternative Medicine 2015: 975632. https://doi.org/10.1155/2015/975632.
McDonald, J.L., A.W. Cripps, P.K. Smith, C.A. Smith, C.C. Xue, and B. Golianu. 2013. The anti-inflammatory effects of acupuncture and their relevance to allergic rhinitis: a narrative review and proposed model. Evidence-based Complementary and Alternative Medicine 2013: 591796. https://doi.org/10.1155/2013/591796.
Wan, F., X. Niu, Y.L. Song, and Y.C. Si. 2016. The role of Chinese herbs and acupuncture on the inflammation reaction after cerebral ischemia. Current Pharmaceutical Design 22: 709–719.
Wei, Y., M. Dong, L. Zhong, J. Liu, Q. Luo, Y. Lv, S. Mo, et al. 2017. Regulation of hypothalamic-pituitary-adrenal axis activity and immunologic function contributed to the anti-inflammatory effect of acupuncture in the OVA-induced murine asthma model. Neuroscience Letters 636: 177–183. https://doi.org/10.1016/j.neulet.2016.11.001.
Zhang, R., L. Lao, K. Ren, and B.M. Berman. 2014. Mechanisms of acupuncture-electroacupuncture on persistent pain. Anesthesiology 120: 482–503. https://doi.org/10.1097/ALN.0000000000000101.
Racke, K., and S. Matthiesen. 2004. The airway cholinergic system: physiology and pharmacology. Pulmonary Pharmacology & Therapeutics 17: 181–198. https://doi.org/10.1016/j.pupt.2004.03.001.
Wessler, I.K., and C.J. Kirkpatrick. 2001. The non-neuronal cholinergic system: an emerging drug target in the airways. Pulmonary Pharmacology & Therapeutics 14: 423–434. https://doi.org/10.1006/pupt.2001.0313.
Gosens, R., J. Zaagsma, H. Meurs, and A.J. Halayko. 2006. Muscarinic receptor signaling in the pathophysiology of asthma and COPD. Respiratory Research 7: 73. https://doi.org/10.1186/1465-9921-7-73.
Ramnarine, S.I., E.B. Haddad, A.M. Khawaja, J.C. Mak, and D.F. Rogers. 1996. On muscarinic control of neurogenic mucus secretion in ferret trachea. Journal of Physiology 494 (Pt 2): 577–586.
Funding
This study was funded by the National Natural Science Foundation of China (No. 81303052) and China Postdoctoral Science Foundation (No. 2012 M 510833).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Ethical Approval
The experimental procedures were carried out in accordance with the Chinese regulations involving animal protection and approved by the animal ethics committee of the Liaoning University of Traditional Chinese Medicine.
Electronic Supplementary Material
Supplementary Table 1
(DOCX 16 kb)
Rights and permissions
About this article
Cite this article
Liu, Yl., Zhang, Ld., Ma, Tm. et al. Feishu Acupuncture Inhibits Acetylcholine Synthesis and Restores Muscarinic Acetylcholine Receptor M2 Expression in the Lung When Treating Allergic Asthma. Inflammation 41, 741–750 (2018). https://doi.org/10.1007/s10753-017-0726-y
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10753-017-0726-y