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CD80 Regulates Th17 Cell Differentiation in Coxsackie Virus B3-Induced Acute Myocarditis

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Abstract

The cluster of differentiation protein complex, CD80/CD86, regulates Th1/Th2 differentiation in autoimmune disease. In order to establish the effects of CD80/CD86 on Th17 cell differentiation in acute viral myocarditis (VMC), we infected C57BL/6 mice with Coxsackie virus B3 (CVB3) and examined the effects of the treatment with anti-CD80/CD86 monoclonal antibodies (mAbs) on Th17 cell differentiation in vivo. The effects of anti-CD80/CD86 mAbs on Th17 cell differentiation were further evaluated in vitro. The treatment with anti-CD80 mAb induced marked suppression of Th17 cell differentiation and ROR-γt mRNA expression, whereas anti-CD86 mAb alone had no effect, both in vivo and in vitro. Our finding that CD80 regulates Th17 differentiation supports the potential utility of anti-CD80 mAb as an effective new immunotherapeutic target in acute VMC.

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Funding

This work was supported by the National Natural Science Foundation of China (81670345).

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Authors and Affiliations

  1. Department of Cardiology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, People’s Republic of China

    Yanlan Huang, Yong Li, Bin Wei, Weifeng Wu & Xingcui Gao

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  1. Yanlan Huang
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  2. Yong Li
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  3. Bin Wei
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  4. Weifeng Wu
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  5. Xingcui Gao
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Corresponding author

Correspondence to Weifeng Wu.

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Animal experiments were implemented in accordance with the protocols approved by the Guangxi Medical University Animal Ethics Committee.

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The authors declare that they have no conflicts of interest.

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Yanlan Huang and Yong Li are equal contributors.

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Huang, Y., Li, Y., Wei, B. et al. CD80 Regulates Th17 Cell Differentiation in Coxsackie Virus B3-Induced Acute Myocarditis. Inflammation 41, 232–239 (2018). https://doi.org/10.1007/s10753-017-0681-7

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  • DOI: https://doi.org/10.1007/s10753-017-0681-7

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