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Inhibition of Endoplasmic Reticulum Stress Alleviates Lung Injury Induced by Brain Death

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Abstract

Brain death (BD) can induce inflammation and injury of organs. Endoplasmic reticulum (ER) stress is associated with a variety of diseases. However, little is known about how ER stress is implicated in brain death (BD)-induced lung injury. In this study, a stable BD rat model was constructed to investigate the role of ER stress on BD-induced lung injury. H&E staining demonstrated that BD can induce lung injury in rats. The results of Western blot and immunohistochemistry showed that apoptosis was observed in the lung tissues of BD rats. And the level of GRP78, p-PERK, p-eIF2α, CHOP, and Caspase-12 was highly expressed in BD rats compared with the control group. Inhibition of ER stress with salubrinal reduced the BD-induced lung inflammation. Moreover, BD-induced increase of NF-κB activity was lowered by inhibition of ER stress. These results suggested that inhibition of ER stress alleviates BD-induced lung inflammation by regulating NF-κB signaling pathway.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant no.81571947) and the Scientific Research Fund of Henan Education Department (grant No. 16A320055).

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Author notes

  1. Hongwei Tang and Jiakai Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital of Zhengzhou University, No.1, East Jian She Road, Zhengzhou, Henan Province, 450052, China

    Hongwei Tang, Jiakai Zhang, Shengli Cao, Bing Yan, Hongbo Fang, Huapeng Zhang, Wenzhi Guo & Shuijun Zhang

  2. Henan Key Laboratory of Digestive Organ Transplantation, No.1, East Jian She Road, Zhengzhou, Henan Province, 450052, China

    Hongwei Tang, Bing Yan, Huapeng Zhang & Shuijun Zhang

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  1. Hongwei Tang
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  4. Bing Yan
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Correspondence to Wenzhi Guo or Shuijun Zhang.

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Tang, H., Zhang, J., Cao, S. et al. Inhibition of Endoplasmic Reticulum Stress Alleviates Lung Injury Induced by Brain Death. Inflammation 40, 1664–1671 (2017). https://doi.org/10.1007/s10753-017-0606-5

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  • DOI: https://doi.org/10.1007/s10753-017-0606-5

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