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Inflammatory and Oxidative Stress Markers in Experimental Allergic Asthma

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Abstract

Ovalbumin-induced allergic lung inflammation (ALI) is a condition believed to be mediated by cytokines, extracellular matrix remodeling, and redox imbalance. In this study, we evaluated pulmonary function together with inflammatory markers as interleukin-4 (IL-4), myeloperoxidase (MPO), eosinophil cells, and redox markers in the lungs of BALB/c mice after ovalbumin (OVA) sensitization and challenge. Our results showed an increase in bronchial hyperresponsiveness stimulated by methacholine (Mch), inflammatory cell influx, especially eosinophils together with an increase of high mobility group box 1 (HMGB1) and altered lipid peroxidation (LP) and antioxidant defenses in the OVA group compared to the control group (p ≤ 0.5). Thus, we demonstrated that OVA-induced ALI altered redox status concomitantly with impaired lung function, which was associated with HMGB1 expression and proteolytic remodeling. Taken together all results found here, we may suggest HMGB1 is an important therapeutic target for asthma, once orchestrates the redox signaling, inflammation, and remodeling that contribute to the disease development.

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Acknowledgements

This work was supported by grant from Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Brazil).

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Authors and Affiliations

  1. Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Renata Tiscoski Nesi, Emanuel Kennedy-Feitosa, Manuella Lanzetti & Samuel Santos Valença

  2. Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense – UNESC, Av. Universitária 1105, Criciúma, SC, 88806-000, Brazil

    Renata Tiscoski Nesi

  3. DeVry/Fanor, Fortaleza, CE, 60191-156, Brazil

    Emanuel Kennedy-Feitosa

  4. Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Mariana Barcellos Ávila, Clarissa Bichara Magalhães, Walter Araújo Zin & Débora Souza Faffe

  5. Programa de Pós-graduação em Biologia Humana e Experimental, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil

    Luís Cristóvão Porto

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  1. Renata Tiscoski Nesi
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  2. Emanuel Kennedy-Feitosa
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  9. Samuel Santos Valença
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Correspondence to Renata Tiscoski Nesi.

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All procedures were done in accordance with international guidelines (NIH) and Brazilian law (the “Arouca” Law) for the use of laboratory animals (Law 11,794 from 10/08/2008), and this study received prior approval from the animal ethics committee of the Federal University of Rio de Janeiro (IBCCF 106)

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Renata Tiscoski Nesi and Emanuel Kennedy-Feitosa contributed equally to this study.

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Nesi, R.T., Kennedy-Feitosa, E., Lanzetti, M. et al. Inflammatory and Oxidative Stress Markers in Experimental Allergic Asthma. Inflammation 40, 1166–1176 (2017). https://doi.org/10.1007/s10753-017-0560-2

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