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Inflammation

, Volume 40, Issue 3, pp 806–817 | Cite as

5-Aminosalicylic Acid Attenuates Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats by Increasing the Expression of Nur77

  • Ling-yue Sun
  • Zong-ye Cai
  • Jun Pu
  • Jian Li
  • Jie-yan ShenEmail author
  • Cheng-de YangEmail author
  • Ben He
ORIGINAL ARTICLE

Abstract

The aim of this study is to investigate the effect of 5-aminosalicylic acid (5-ASA) on monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH) in rats and its molecular mechanism. Sixty male Sprague–Dawley rats (250–300 g) were evenly randomized into six groups: control group; PAH group induced by MCT intraperitoneal injection (50 mg/kg) on day 1; and four PAH groups treated for 30 days from day 2 with 5-ASA at 50 (5-ASA-50 group), 100 (5-ASA-100 group), 150 (5-ASA-150 group), and 200 mg/kg/day (5-ASA-200 group), respectively. Body mass, weight increment, survival rates, pulmonary artery pressure (PAP), right ventricular hypertrophy index (RVHI), and the signal pathway regulated by 5-ASA were assessed. (1) Compared with the control group, the PAH group had lower body mass and weight increment, and relative to the latter, 5-ASA-treated groups had larger body mass and weight increment except for groups 5-ASA-150 and 5-ASA-200 and greater overall survival rates; (2) SPAP, DPAP, MPAP, and RVHI in 5-ASA-treated groups, except for MPAP and RVHI in 5-ASA-200 group, were lower than those in the PAH group; (3) compared with the PAH group, Nur77 expression in the pulmonary arteries of 5-ASA-treated groups was increased; and (4) expression of inflammatory mediators (NF-κB p65) was lower, while that of IκBα was higher in the pulmonary arteries of 5-ASA-treated groups and control group than that in the PAH group (all P < 0.05). 5-ASA attenuates PAH in MCT-injected rats, reducing pulmonary arterial pressures and right ventricular hypertrophy and improving survival rates, via the Nur77-NF-κB/IκBα pathway involved in modulating the pulmonary vascular remodeling.

KEY WORDS

5-aminosalicylic acid pulmonary arterial hypertension pulmonary vascular remodeling Nur77 MCT 

Notes

Compliance with Ethical Standards

Funding

This work was supported by research fund from National Natural Science Foundation of China (CN) (81570040).

Conflict of Interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Cardiology, Ren Ji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of CardiologyRenji Hospital (South), School of Medicine, Shanghai Jiao Tong UniversityShanghaiChina
  3. 3.Department of CardiologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina
  4. 4.Department of Rheumatology, Rui Jin Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina

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