Picroside II Protects Rat Lung and A549 Cell Against LPS-Induced Inflammation by the NF-κB Pathway
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Picroside II is the main active ingredient in the root department of Chinese medicine Picrorhiza scrophulariiflora which has been proved to have beneficial effects on health, such as ameliorating the cerebral ischemia and protecting the liver. However, its effects on acute lung injury remain unclear. The purpose of the study was to evaluate the effects of picroside II on acute lung injury in mice and the inflammation in A549 cells which are lipopolysaccharide (LPS) induced. We evaluated the levels of tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1β), and interleukin-6 (IL-6) in vivo and vitro by enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qRT-PCR). Results showed picroside II significantly decreased the concentrations of TNF-α, IL-1β, and IL-6 in cells and mice. In addition, Western blot and immunofluorescence analysis indicated that picroside II suppressed the activation of p65 NF-κB signaling pathway compared with LPS stimulation. In the acute lung injury model of mice, after picroside II treatment, the pathologic changes of lung tissues had been alleviated and lung wet/dry weight ratio was decreased. In summary, picroside II showed the promising effects of anti-inflammation in cells and animals.
KEY WORDSpicroside II cytokines NF-κB anti-inflammation
B.Y.S., C.X.Z., C.Z.C., and H.H.F. contributed to the design. B.Y.S., C.X.Z., Z.L., Y.L., and Y.T. did the data collection. B.Y.S., C.X.Z., and H.H.F. did the analysis. B.Y.S. did the writing of the article. C.X.Z., C.Z.C., and H.H.F. did the revisions to the article. This work was supported by grants from the National Nature Science Foundation of China (nos. 31572347 and 31372478).
Compliance with Ethical Standards
All animal experiments were performed in accordance with the National Institutes of Health (NIH) guide for the Care and Use of Laboratory Animals and approved by the Jilin University animal administration committee.
Conflict of Interest
The authors declare that they have no conflict of interest.
- 12.Siomek, A. 2012. NF-κB signaling pathway and free radical impact. Acta Biochimica 59: 323–331.Google Scholar
- 13.Wang, L., X.H. Liu, H. Chen, and Z.Y. Chen. 2015. Picroside II protects rat kidney against ischemia/reperfusion-induced oxidative stress and inflammation by the TLR4/NF-κB pathway. Experimental and Therapeutic Medcine 9: 1253–1258.Google Scholar
- 20.Fiorentino, D.F., A. Zlotnik, T.R. Mosmann, M. Howard, and A. O’Garra. 1991. IL-10 inhibits cytokine production by activated macrophages. Journal of Immunology 146: 3444–3451.Google Scholar
- 28.Xia, Y.F., B.Q. Ye, Y.D. Li, J.G. Wang, X.J. He, X. Lin, X. Yao, D. Ma, A. Slungaard, R.P. Hebbel, N.S. Key, and J.G. Geng. 2004. Andrographolide attenuates inflammation by inhibition of NF -κB activation through covalent modification of reduced cysteine 62 of p50. Journal of Immunology 173: 4207–4217.CrossRefGoogle Scholar