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Veratric Acid Inhibits LPS-Induced IL-6 and IL-8 Production in Human Gingival Fibroblasts

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Abstract

Veratric acid, one of the major benzoic acid isolated from vegetables and fruits, has been reported to have anti-inflammatory activity. The purpose of this study was to investigate the anti-inflammatory effects of veratric acid on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). HGFs were pretreated with veratric acid 1 h before LPS stimulation. The productions of IL-6 and IL-8 were detected by ELISA. The expression of iNOS, COX-2, PI3K, AKT, and NF-κB were detected by western blotting. The results showed that veratric acid inhibited LPS-induced IL-6 and IL-8 production, as well as iNOS and COX-2 expression. Veratric acid also inhibited LPS-induced NF-κB activation. In addition, veratric acid was found to suppress LPS-induced PI3K and AKT phosphorylation. In conclusion, the anti-inflammatory mechanism of veratric acid is due to its ability to inhibit PI3K/Akt/NF-κB signaling pathway.

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Acknowledgments

This study was supported by “Health Science and Technology Development Program Project of Jinan” (no. 2011-14) and “Science and Technology Development Program Project of Jinan” (no. 201401082).

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, 250001, China

    Qi-bao Wang, Zuo-de Gong & Yi Du

  2. Dental Implant Center, Jinan Stomatological Hospital, Jinan, Shandong, 250001, China

    Lan-ying Sun

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  1. Qi-bao Wang
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  2. Lan-ying Sun
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  3. Zuo-de Gong
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  4. Yi Du
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Correspondence to Yi Du.

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Wang, Qb., Sun, Ly., Gong, Zd. et al. Veratric Acid Inhibits LPS-Induced IL-6 and IL-8 Production in Human Gingival Fibroblasts. Inflammation 39, 237–242 (2016). https://doi.org/10.1007/s10753-015-0243-9

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  • DOI: https://doi.org/10.1007/s10753-015-0243-9

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