Abstract
Kidney allograft biopsies were analyzed for the presence of B cell clusters/aggregates using CD20 staining. Few B cells were found in the diffuse interstitial infiltrates, but clusters of B cells were found in nodular infiltrates. These nodular infiltrates were smaller shortly after transplantation, and their size increased over time. At the time of clinical rejection, the nodules often presented as tertiary lymphoid structures (TLS) with lymphoid-like follicles. The presence of small B cell clusters during the first 2 months after transplantation was not associated with early rejection. Even in animals that did not reject their allograft, TLS-like structures were present and could disappear over time. Although TLS were more often found in samples with interstitial fibrosis and tubular atrophy (IFTA), TLS were also present in samples without IFTA. The presence and density of clusters resembling tertiary lymphoid structures most likely reflect an ongoing immune response inside the graft and do not necessarily signify a poor graft outcome or IFTA.
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Abbreviations
- CsA:
-
Cyclosporine A
- IFTA:
-
Interstitial fibrosis and tubular atrophy
- NHP:
-
Non-human primate
- TLS:
-
Tertiary lymphoid structure
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The authors of this manuscript would like to thank Henk van Westbroek for the artwork.
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Research involving animals.
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The authors declare that they have no competing interests.
Authors Contribution
MJ analyzed data and wrote the paper; JAMW performed research and collected data; KH and BA’tH were critically involved in the research and drafting of the manuscript.
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Jonker, M., Wubben, J.A.M., ‘t Hart, B.A. et al. Lymphoid-Like Structures with Distinct B Cell Areas in Kidney Allografts are not Predictive for Graft Rejection. A Non-human Primate Study. Inflammation 38, 2191–2202 (2015). https://doi.org/10.1007/s10753-015-0202-5
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DOI: https://doi.org/10.1007/s10753-015-0202-5