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P2X7 Receptor Expression in Peripheral Blood Monocytes Is Correlated With Plasma C-Reactive Protein and Cytokine Levels in Patients With Type 2 Diabetes Mellitus: a Preliminary Report

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Abstract

Chronic inflammation plays a major role in development of type 2 diabetes mellitus (T2DM). C-reactive protein (CRP) and inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β) are directly involved in the occurrence of insulin resistance. Increased extracellular ATP levels can amplify the inflammatory response in vivo via the P2X7 receptor. The present study aimed to assess the relationship between P2X7 receptor expression in human peripheral blood monocytes and plasma levels of TNF-α, IL-1β, and CRP in T2DM patients. The results showed the association of increased P2X7 receptor expression of monocytes with high serum CRP, TNF-α, and IL-1β levels. TNF-α and IL-1β levels were lowest in healthy subjects; in T2DM patients, these inflammatory markers were less abundant in individuals with normal CRP levels compared to those with high CRP contents. In contrast, IL-10 levels in T2DM patients with high CRP levels were dramatically decreased. P2X7 receptor expression in monocytes from T2DM patients with high CRP levels was significantly increased in comparison with healthy individuals and T2DM patients with normal CRP levels. These findings indicated that P2X7 receptor in peripheral blood monocytes may be involved in the pathological changes of T2DM, particularly affecting patients with high CRP levels.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Nos. 81171184, 31060139, 81200853, and 81360140), the Natural Science Foundation of Jiangxi Province (Nos. 20142BAB205028 and 20142BAB215027), the second batch of Jiangxi Province “Gan Po Excellence Talent 555 Project”—leading talent project, and the Educational Department of Jiangxi Province (Nos. GJJ14319 and GJJ13155).

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China

    Hong Wu, Huangui Xiong, Shuangmei Liu, Guilin Li, Lili Guo, Shouyu Wang, Yun Xue, Bing Wu, Lichao Peng, Miaomiao Song, Guodong Li & Shangdong Liang

  2. First Affiliated Hospital, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China

    Yijun Nie

  3. Jiangxi University of Finance and Economics, Nanchang, Jiangxi, 330006, People’s Republic of China

    An Huang

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  1. Hong Wu
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Correspondence to Shangdong Liang.

Additional information

Hong Wu, Yijun Nie, and Huangui Xiong are joint first authors.

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Wu, H., Nie, Y., Xiong, H. et al. P2X7 Receptor Expression in Peripheral Blood Monocytes Is Correlated With Plasma C-Reactive Protein and Cytokine Levels in Patients With Type 2 Diabetes Mellitus: a Preliminary Report. Inflammation 38, 2076–2081 (2015). https://doi.org/10.1007/s10753-015-0189-y

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  • DOI: https://doi.org/10.1007/s10753-015-0189-y

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