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Renoprotective Effect of Humic Acid on Renal Ischemia-Reperfusion Injury: An Experimental Study in Rats

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Abstract

Humic acid is an antioxidant molecule used in agriculture and livestock breeding, as well as in medicine. Our aim was to investigate the potential renoprotective effects of humic acid in a renal ischemia reperfusion model. Twenty-one rats were randomly divided into three equal groups. Intraperitoneal serum or humic acid was injected at 1, 12, and 24 h. Non-ischemic group I was evaluated as sham. The left renal artery was clamped in serum (group II) and intraperitoneal humic acid (group III) to subject to left renal ischemic reperfusion procedure. Ischemia and reperfusion time was 60 min for each. Total antioxidant status, total oxidative status, oxidative stress index, and ischemia-modified albumin levels were analyzed biochemically from the serum samples. Kidneys were evaluated histopatologically and immunohistochemically. Biochemical results showed that total oxidative status, ischemia-modified albumin, and oxidative stress index levels were significantly decreased, but total antioxidant status was increased in the humic acid group (III) compared with the ischemia group (II) On histopathological examination, renal tubular dilatation, tubular cell damage and necrosis, dilatation of Bowman’s capsule, hyaline casts, and tubular cell spillage were decreased in the humic acid group (III) compared with the ischemia group (II). Immunohistochemical results showed that apoptosis was deteriorated in group III. Renal ischemia reperfusion injury was attenuated by humic acid administration. These observations indicate that humic acid may have a potential therapeutic effect on renal ischemia reperfusion injury by preventing oxidative stress.

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Acknowledgments

This work was supported by the Research Fund of Çanakkale Onsekiz Mart University. Project Number 159.

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Authors and Affiliations

  1. Faculty of Medicine, Department of Urology, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Alpaslan Akbas, Murat Tolga Gulpinar & Eyup Burak Sancak

  2. Faculty of Medicine, Department of Pharmacology, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Coskun Silan

  3. Faculty of Medicine, Department of Pathology, Medeniyet University, Istanbul, Turkey

    Sidika Seyma Ozkanli

  4. Faculty of Medicine, Department of Biochemistry, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Dilek Ulker Cakir

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  1. Alpaslan Akbas
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  2. Coskun Silan
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  3. Murat Tolga Gulpinar
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  4. Eyup Burak Sancak
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  5. Sidika Seyma Ozkanli
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  6. Dilek Ulker Cakir
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Correspondence to Alpaslan Akbas.

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Akbas, A., Silan, C., Gulpinar, M.T. et al. Renoprotective Effect of Humic Acid on Renal Ischemia-Reperfusion Injury: An Experimental Study in Rats. Inflammation 38, 2042–2048 (2015). https://doi.org/10.1007/s10753-015-0185-2

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  • DOI: https://doi.org/10.1007/s10753-015-0185-2

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