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Elimination Half-Lives of Acute Phase Proteins in Rats and Beagle Dogs During Acute Inflammation

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ABSTRACT

The half-lives of typical acute phase proteins in rats and beagle dogs during acute inflammation were investigated. Acute inflammation was induced by injection of turpentine oil in rats and administration of indomethacin in beagle dogs. Serum concentrations of α2-macroglobulin (α2M) and C-reactive protein (CRP) were measured by enzyme-linked immunosorbent assay and α1-acid glycoprotein (AAG) was measured by single radial immunodiffusion. Half-life was calculated as 0.693/elimination rate constant (K). The mean half-lives in the terminal elimination phase of α2M and AAG were 68.1 and 164.8 h, respectively. The half-life of AAG was significantly longer than that of α2M. Mean half-lives in the terminal elimination phase of CRP and AAG were 161.9 and 304.4 h, respectively. The half-life of AAG was significantly longer than that of CRP in beagle dogs. No significant differences in the half-life of AAG were observed between rats and beagle dogs. Furthermore, serum concentrations in the terminal elimination phase could be simulated with the K data acquired in this study.

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ACKNOWLEDGMENTS

This research was partially supported by a research project grant awarded by Azabu University.

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Authors and Affiliations

  1. Graduate School of Environmental and Health Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku, Sagamihara, Kanagawa, 252-5201, Japan

    Takashi Kuribayashi, Tetsuro Seita & Shizuo Yamamoto

  2. Department of Human Health Care, Tohto College of Health Sciences, 4-2-11 Kamishiba-Cho Nishi, Fukaya, Saitama, 366-0052, Japan

    Eiichi Momotani

  3. Laboratory of Physiology, Department of Food and Nutrition, Kamakura Women’s University, 6-1-3 Ofuna, Kamakura, Kanagawa, 247-8512, Japan

    Shunsuke Yamazaki

  4. Laboratory of Immunology, Faculty of Life and Environmental Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku, Sagamihara, Kanagawa, 252-5201, Japan

    Kohei Hagimori

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  1. Takashi Kuribayashi
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  2. Tetsuro Seita
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  4. Shunsuke Yamazaki
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  6. Shizuo Yamamoto
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Correspondence to Shizuo Yamamoto.

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Kuribayashi, T., Seita, T., Momotani, E. et al. Elimination Half-Lives of Acute Phase Proteins in Rats and Beagle Dogs During Acute Inflammation. Inflammation 38, 1401–1405 (2015). https://doi.org/10.1007/s10753-015-0114-4

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