Abstract
Although brain disorders are not the main indication for radon therapy, our previous study suggested that radon inhalation therapy might mitigate brain disorders. In this study, we assessed whether radon inhalation protects against transient global cerebral ischemic injury in gerbils. Gerbils were treated with inhaled radon at a concentration of 2,000 Bq/m3 for 24 h. After radon inhalation, transient global cerebral ischemia was induced by bilateral occlusion of the common carotid artery. Results showed that transient global cerebral ischemia induced neuronal damage in hippocampal CA1, and the number of damaged neurons was significantly increased compared with control. However, radon treatment inhibited ischemic damage. Superoxide dismutase (SOD) activity in the radon-treated gerbil brain was significantly higher than that in sham-operated gerbils. These findings suggested that radon inhalation activates antioxidative function, especially SOD, thereby inhibiting transient global cerebral ischemic injury in gerbils.
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REFERENCES
Gilgun-Sherki, Y., Z. Rosenbaum, E. Melamed, and D. Offen. 2002. Antioxidant therapy in acute central nervous system injury: current state. Pharmacological Reviews 54: 271–284.
Murakami, K., T. Kondo, C.J. Epstein, and P.H. Chan. 1997. Overexpression of CuZn-superoxide dismutase reduces hippocampal injury after global ischemia in transgenic mice. Stroke 28: 1797–1804.
Chan, P.H. 2001. Reactive oxygen radicals in signaling and damage in the ischemic brain. Journal of Cerebral Blood Flow and Metabolism 21: 2–14.
Chan, P.H., M. Kawase, K. Murakami, S.F. Chen, Y. Li, B. Calagui, L. Reola, E. Carlson, and C.J. Epstein. 1998. Overexpression of SOD1 in transgenic rats protects vulnerable neurons against ischemic damage after global cerebral ischemia and reperfusion. The Journal of Neuroscience 18: 8292–8299.
Keller, J.N., M.S. Kindy, F.W. Holtsberg, D.K. St, H.C. Clair, A. Yen, S.M. Germeyer, A.J. Steiner, J.B. Hutchins Bruce-Keller, and M.P. Mattson. 1998. Mitochondrial manganese superoxide dismutase prevents neural apoptosis and reduces ischemic brain injury: suppression of peroxynitrite production, lipid peroxidation, and mitochondrial dysfunction. The Journal of Neuroscience 18: 687–697.
Sheng, H., R.D. Bart, T.D. Oury, R.D. Pearlstein, J.D. Crapo, and D.S. Warner. 1999. Mice overexpressing extracellular superoxide dismutase have increased resistance to focal cerebral ischemia. Neuroscience 88: 185–191.
Yamaoka, K., S. Kojima, M. Takahashi, T. Nomura, and K. Iriyama. 1998. Change of glutathione peroxidase synthesis along with that of superoxide dismutase synthesis in mice spleens after low-dose X-ray irradiation. Biochimica et Biophysica Acta 1381: 265–270.
Yamaoka, K., S. Kojima, and T. Nomura. 1999. Changes of SOD-like substances in mouse organs after low-dose X-ray irradiation. Physiological Chemistry and Physics and Medical NMR 31: 23–28.
Yamaoka, K. 2006. Activation of antioxidant system by low dose radiation and its applicable possibility for treatment of reactive oxygen species-related diseases. Journal of Clinical Biochemistry and Nutrition 39: 114–133.
Kataoka, T. 2013. Study of antioxidative effects and anti-inflammatory effects in mice due to low-dose X-irradiation or radon inhalation. Journal of Radiation Research 54: 587–596.
Kataoka, T., Y. Mizuguchi, M. Yoshimoto, T. Taguchi, and K. Yamaoka. 2007. Inhibitory effects of prior low-dose X-irradiation on ischemia-reperfusion injury in mouse paw. Journal of Radiation Research 48: 505–513.
Yoshimoto, M., T. Kataoka, T. Toyota, T. Taguchi, and K. Yamaoka. 2012. Inhibitory effects of prior low-dose X-irradiation on cold-induced brain injury in mouse. Inflammation 35: 89–97.
Yamaoka, K., F. Mitsunobu, K. Hanamoto, S. Mori, Y. Tanizaki, and K. Sugita. 2004. Study on biologic effects of radon and thermal therapy on osteoarthritis. The Journal of Pain 5: 20–25.
Mitsunobu, F., K. Yamaoka, K. Hanamoto, S. Kojima, Y. Hosaki, K. Ashida, K. Sugita, and Y. Tanizaki. 2003. Elevation of antioxidant enzymes in the clinical effects of radon and thermal therapy for bronchial asthma. Journal of Radiation Research 44: 95–99.
Ishimori, Y., F. Mitsunobu, K. Yamaoka, H. Tanaka, T. Kataoka, and A. Sakoda. 2010. Development of a radon test facility for small animals. Japanese Journal of Health Physics 45: 65–71.
Kataoka, T., A. Sakoda, Y. Ishimori, T. Toyota, Y. Nishiyama, H. Tanaka, F. Mitsunobu, and K. Yamaoka. 2011. Study of the response of superoxide dismutase in mouse organs to radon using a new large-scale facility for exposing small animals to radon. Journal of Radiation Research 52: 775–781.
Kataoka, T., J. Teraoka, A. Sakoda, Y. Nishiyama, K. Yamato, M. Monden, Y. Ishimori, T. Nomura, T. Taguchi, and K. Yamaoka. 2012. Protective effects of radon inhalation on carrageenan-induced inflammatory paw edema in mice. Inflammation 35: 713–722.
Baehner, R.L., S.K. Murrmann, J. Davis, and R.B. Johnston. 1975. The role of superoxide anion and hydrogen peroxide in phagocytosis-associated oxidative metabolic reactions. The Journal of Clinical Investigation 56: 571–576.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254.
Yamato, K., T. Kataoka, Y. Nishiyama, T. Taguchi, and K. Yamaoka. 2013. Antinociceptive effects of radon inhalation on formalin-induced inflammatory pain in mice. Inflammation 36: 355–363.
Yamato, K., T. Kataoka, T. Taguchi Nishiyama, and K. Yamaoka. 2013. Preventive and curative effects of radon inhalation on chronic constriction injury-induced neuropathic pain in mice. European Journal of Pain 17: 480–492.
Kataoka, T., Y. Nishiyama, T. Toyota, M. Yoshimoto, A. Sakoda, Y. Ishimori, Y. Aoyama, T. Taguchi, and K. Yamaoka. 2011. Radon inhalation protects mice from carbon-tetrachloride-induced hepatic and renal damage. Inflammation 34: 559–567.
Kataoka, T., Y. Nishiyama, K. Yamato, J. Teraoka, Y. Morii, A. Sakoda, Y. Ishimori, T. Taguchi, and K. Yamaoka. 2012. Comparative study on the inhibitory effects of antioxidant vitamins and radon on carbon tetrachloride-induced hepatopathy. Journal of Radiation Research 53: 830–889.
Kataoka, T., K. Yamato, Y. Nishiyama, Y. Morii, R. Etani, Y. Takata, K. Hanamoto, A. Kawabe, A. Sakoda, Y. Ishimori, T. Taguchi, and K. Yamaoka. 2012. Comparative study on the inhibitory effects of α-tocopherol and radon on carbon tetrachloride-induced renal damage. Renal Failure 34: 1181–1187.
Urabe, T., Y. Yamasaki, N. Hattori, M. Yoshikawa, K. Uchida, and Y. Mizuno. 2000. Accumulation of 4-hydroxynonenal-modified proteins in hippocampal CA1 pyramidal neurons precedes delayed neuronal damage in the gerbil brain. Neuroscience 100: 241–250.
Wang, Q., A.Y. Sun, A. Simonyi, M.D. Jensen, P.B. Shelat, G.E. Rottinghaus, R.S. MacDonald, D.K. Miller, D.E. Lubahn, G.A. Weisman, and G.Y. Sun. 2005. Neuroprotective mechanisms of curcumin against cerebral ischemia-induced neuronal apoptosis and behavioral deficits. Journal of Neuroscience Research 82: 138–148.
Wattanapitayakul, S.K., and J.A. Bauer. 2001. Oxidative pathways in cardiovascular disease: roles, mechanisms, and therapeutic implications. Pharmacology & Therapeutics 89: 187–206.
Antonawich, F.J., H.J. Federoff, and J.N. Davis. 1999. BCL-2 transduction, using a herpes simplex virus amplicon, protects hippocampal neurons from transient global ischemia. Experimental Neurology 156: 130–137.
Hara, A., T. Iwai, M. Niwa, T. Uematsu, N. Yoshimi, T. Tanaka, and H. Mori. 1996. Immunohistochemical detection of Bax and Bcl-2 proteins in gerbil hippocampus following transient forebrain ischemia. Brain Research 711: 249–253.
Lorrioa, S., P. Negredo, J.M. Roda, A.G. García, and M.G. López. 2009. Effects of memantine and galantamine given separately or in association, on memory and hippocampal neuronal loss after transient global cerebral ischemia in gerbils. Brain Research 1254: 128–137.
Park, J.H., H.S. Joo, K.Y. Yoo, B.N. Shin, I.H. Kim, C.H. Lee, J.H. Choi, K. Byun, B. Lee, S.S. Lim, M.J. Kim, and M.H. Won. 2011. Extract from terminalia chebula seeds protect against experimental ischemic neuronal damage via maintaining SODs and BDNF levels. Neurochemical Research 36: 2043–2050.
Yoon, D.K., K.Y. Yoo, I.K. Hwang, J.J. Lee, J.H. Kim, T.C. Kang, and M.H. Won. 2006. Comparative study on Cu, Zn-SOD immunoreactivity and protein levels in the adult and aged hippocampal CA1 region after ischemia–reperfusion. Brain Research 1092: 214–219.
ACKNOWLEDGMENTS
This research was supported by a Grant-In-Aid for Young Scientists (B) from MEXT (No. 25820449).
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Kataoka, T., Etani, R., Takata, Y. et al. Radon Inhalation Protects Against Transient Global Cerebral Ischemic Injury in Gerbils. Inflammation 37, 1675–1682 (2014). https://doi.org/10.1007/s10753-014-9896-z
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DOI: https://doi.org/10.1007/s10753-014-9896-z