The present work investigates the anti-inflammatory activity of alpha-linolenic acid (ALA) and linoleic acid (LA) using computational and experimental analysis. The binding affinity of ALA and LA was appraised for cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), and 5-lipoxygenase (5-LOX) using AutoDock 4.2 and AutoDock Vina 1.1.2. Anti-inflammatory activity of ALA (2 and 4 ml/kg, i.p.) (55.65 % v/v) and LA (2 and 4 ml/kg, i.p.) (55 % v/v) was further assayed using the rat paw edema test against a variety of phlogistic agents including carrageenan, arachidonic acid, prostaglandin, and leukotriene, respectively. ALA (2 and 4 ml/kg, i.p.) and LA (2 and 4 ml/kg, i.p.) were further tested for their efficacy against complete Freund’s adjuvant (CFA)-induced (0.05 ml) arthritis in albino rats. Following CFA-induced arthritis, ALA and LA were tested for their inhibitory proficiency against COX-1, COX-2, and 5-LOX in vitro. The present study commends that the anti-inflammatory potential of ALA could be attributed to COX inhibition, in particular, COX-2.
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Authors are thankful to Arbro Pharmaceuticals, Kirti Nagar, New Delhi, for providing the drugs mentioned in the text as gift sample. RA is receiving SRF from Ministry of Human Resource and Development, Government of India, for pursuing the Doctoral program.
Conflict of Interest
The authors declare that they do not have conflicts of interest.
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Anand, R., Kaithwas, G. Anti-inflammatory Potential of Alpha-Linolenic Acid Mediated Through Selective COX Inhibition: Computational and Experimental Data. Inflammation 37, 1297–1306 (2014). https://doi.org/10.1007/s10753-014-9857-6
- alpha-linolenic acid
- computational docking
- linoleic acid
- structure-based docking