, Volume 37, Issue 4, pp 1297–1306 | Cite as

Anti-inflammatory Potential of Alpha-Linolenic Acid Mediated Through Selective COX Inhibition: Computational and Experimental Data

  • Richa Anand
  • Gaurav KaithwasEmail author


The present work investigates the anti-inflammatory activity of alpha-linolenic acid (ALA) and linoleic acid (LA) using computational and experimental analysis. The binding affinity of ALA and LA was appraised for cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), and 5-lipoxygenase (5-LOX) using AutoDock 4.2 and AutoDock Vina 1.1.2. Anti-inflammatory activity of ALA (2 and 4 ml/kg, i.p.) (55.65 % v/v) and LA (2 and 4 ml/kg, i.p.) (55 % v/v) was further assayed using the rat paw edema test against a variety of phlogistic agents including carrageenan, arachidonic acid, prostaglandin, and leukotriene, respectively. ALA (2 and 4 ml/kg, i.p.) and LA (2 and 4 ml/kg, i.p.) were further tested for their efficacy against complete Freund’s adjuvant (CFA)-induced (0.05 ml) arthritis in albino rats. Following CFA-induced arthritis, ALA and LA were tested for their inhibitory proficiency against COX-1, COX-2, and 5-LOX in vitro. The present study commends that the anti-inflammatory potential of ALA could be attributed to COX inhibition, in particular, COX-2.


alpha-linolenic acid computational docking cyclooxygenase inflammation linoleic acid lipoxygenase structure-based docking 



Authors are thankful to Arbro Pharmaceuticals, Kirti Nagar, New Delhi, for providing the drugs mentioned in the text as gift sample. RA is receiving SRF from Ministry of Human Resource and Development, Government of India, for pursuing the Doctoral program.

Conflict of Interest

The authors declare that they do not have conflicts of interest.

Supplementary material

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Supplementary Figure I (DOCX 22 kb)
10753_2014_9857_MOESM2_ESM.docx (61 kb)
Supplementary Figure II (DOCX 60 kb)
10753_2014_9857_MOESM3_ESM.docx (14 kb)
Supplementary Table I (DOCX 14 kb)
10753_2014_9857_MOESM4_ESM.docx (15 kb)
Supplementary Table II (DOCX 15 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Division of Applied Sciences and Indo-Russian Centre for BiotechnologyIndian Institute of Information TechnologyAllahabadIndia
  2. 2.Department of Pharmaceutical SciencesBabasaheb Bhimrao Ambedkar University (A Central University)LucknowIndia

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