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Effect of IL-15 and Natural Killer Cells on Osteoclasts and Osteoblasts in a Mouse Coculture

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Abstract

This study analyzes the effect of interleukin-15 (IL-15) on osteoclast formation using a coculture of mouse osteoblasts and bone marrow cells (BMCs) stimulated with prostaglandin E2 (PGE2), which both have important role in rheumatoid arthritis (RA) and periodontal disease (PD). BMCs isolate lacking T (BMT−) or NK (BMNK−) cells, BMCs with no cells removed (BMT+NK+), purified NK cells, and purified T cells were each cocultured with osteoblasts in the presence or absence of PGE2 and/or IL-15. The number of both osteoclasts and osteoblasts was decreased by IL-15 in a dose-dependent manner in BMT+NK+, BMT−. However, the reductions were improved in BMNK−. The expression of caspase3 in osteoblasts cocultured with NK cells was increased in a dose-dependent manner by IL-15. IL-15 stimulates apoptosis of osteoblasts via activation of NK cells. Since osteoblasts have an important role in bone formation, IL-15 may be an inflammatory bone destructive factor in RA and PD.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (20791628) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Tokyo, Japan, and a Aichi Gakuin University High-Tech Research Center (Aichi, Japan) Project for Private Universities, a matching fund subsidy from MEXT.

Conflict of Interest

All authors declare that they have no conflicts of interest to disclose in relation to the publication of this work.

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Authors and Affiliations

  1. Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Suemoridori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan

    Hiroaki Takeda, Takeshi Kikuchi, Kyoko Soboku, Iichiro Okabe, Hiroki Mizutani, Akio Mitani, Yuichi Ishihara & Toshihide Noguchi

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  1. Hiroaki Takeda
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  2. Takeshi Kikuchi
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Correspondence to Takeshi Kikuchi.

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Takeda, H., Kikuchi, T., Soboku, K. et al. Effect of IL-15 and Natural Killer Cells on Osteoclasts and Osteoblasts in a Mouse Coculture. Inflammation 37, 657–669 (2014). https://doi.org/10.1007/s10753-013-9782-0

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