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1,8-Cineol Attenuates LPS-Induced Acute Pulmonary Inflammation in Mice

Abstract

Eucalyptol, also known as 1,8-cineol, is a monoterpene and has been shown to exert anti-inflammatory and antioxidant effect. It is traditionally used to treat respiratory disorders due to its secretolytic properties. In the present study, we evaluated the effect of 1,8-cineol on pulmonary inflammation in a mouse model of acute lung injury. We found that 1,8-cineol significantly decreased the level of TNF-α and IL-1β, and increased the level of IL-10 in lung tissues after acute lung injury induced by lipopolysaccharide (LPS). It also reduced the expression of nuclear factor kappa B (NF-κB) p65 and toll-like receptor 4 (TLR4), and myeloperoxidase activity in lung tissues. In addition, 1,8-cineol reduced the amounts of inflammatory cells in bronchoalveolar lavage fluid (BALF), including neutrophils and macrophages, and significantly decreased the protein content in BALF and the lung wet/dry weight (W/D) ratio. Its effect on LPS-induced pulmonary inflammation was associated with suppression of TLR4 and NF-κB expressions. Our results provide evidence that 1,8-cineol inhibits acute pulmonary inflammation, indicating its potential for the treatment of acute lung injury.

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Correspondence to Chunzhen Zhao.

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Zhao, C., Sun, J., Fang, C. et al. 1,8-Cineol Attenuates LPS-Induced Acute Pulmonary Inflammation in Mice. Inflammation 37, 566–572 (2014). https://doi.org/10.1007/s10753-013-9770-4

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  • DOI: https://doi.org/10.1007/s10753-013-9770-4

KEY WORDS

  • 1,8-cineol
  • lipopolysaccharide
  • inflammation
  • acute lung injury