, Volume 37, Issue 2, pp 500–511 | Cite as

Grape Seed Proanthocyanidin Extract Protects Against Carrageenan-Induced Lung Inflammation in Mice Through Reduction of Pro-inflammatory Markers and Chemokine Expressions

  • Sheikh Fayaz AhmadEmail author
  • Khairy M. A. Zoheir
  • Hala E. Abdel-Hamied
  • Sabry M. Attia
  • Saleh A. Bakheet
  • Abdelkader E. Ashour
  • Adel R. A. Abd-AllahEmail author


Proanthocyanidins are the most abundant phenolic compounds and have been reported to exert anti-inflammatory actions. The aim of this study was to investigate the effects of grape seed proanthocyanidin extract (GSPE) in a mouse model of carrageenan-induced pleurisy. Following the induction of pleurisy using λ-carrageenan (Cg, 1 %), GSPE (25, 50 and 100 mg/kg) was administered per-oral (p.o.), and the glucocorticoid-induced tumour necrosis factor receptor (GITR), IL-17A expressing cells and other markers, such as cytokines (Th1/Th2 and Th17), were studied. We evaluate the effects of GSPE on the mRNA expression of pro-inflammatory and anti-inflammatory mediators. The results illustrated that the cell numbers of IL-17A and GITR expressing cells and the cytokine levels in Th1/Th17 cells were markedly increased in the Cg-group, whereas the cytokines produced by Th2 cells were significantly decreased in the same group. Treatment with GSPE reversed these effects. Histological examinations revealed anti-inflammatory effects of GSPE.


carrageenan cytokines grape seed proanthocyanidin extract GITR pleural exudate lung 



The authors are grateful to the College of Pharmacy Research Center and the Deanship of Scientific Research, King Saud University, Riyadh, Saudi Arabia, for the financial support.

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sheikh Fayaz Ahmad
    • 1
    Email author
  • Khairy M. A. Zoheir
    • 1
    • 2
  • Hala E. Abdel-Hamied
    • 3
  • Sabry M. Attia
    • 1
    • 4
  • Saleh A. Bakheet
    • 1
  • Abdelkader E. Ashour
    • 1
  • Adel R. A. Abd-Allah
    • 1
    • 4
    Email author
  1. 1.Department of Pharmacology and Toxicology, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Cell BiologyNational Research CentreCairoEgypt
  3. 3.Department of Pathology, Faculty of Medicine (Girls)Al-Azhar UniversityCairoEgypt
  4. 4.Department of Pharmacology and Toxicology, College of PharmacyAl-Azhar UniversityCairoEgypt

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