In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-κB were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-κB protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice.
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Rubenfeld, G.D. 2003. Epidemiology of acute lung injury. Critical Care Medicine 31: S276–S284.
Zhang, X.M., K.J. Song, H.Z. Xiong, H.Y. Li, X. Chu, and X.M. Deng. 2009. Protective effect of florfenicol on acute lung injury induced by lipopolysaccharide in mice. International Immunopharmacology 9: 1525–1529.
Matthay, M.A., G.A. Zimmerman, C. Esmon, J. Bhattacharya, B. Coller, C.M. Doerschuk, et al. 2003. Future research directions in acute lung injury summary of a national heart lung and blood institute working group. American Journal of Respiratory and Critical Care Medicine 167: 1027–1035.
Chen, Z., X. Zhang, X. Chu, X. Zhang, K. Song, Y. Jiang, L. Yu, and X. Deng. 2010. Preventive effects of valnemulin on lipopolysaccharide-induced acute lung injury in mice. Inflammation 33: 306–314.
Hewett, J.A., and R.A. Roth. 1993. Hepatic and extrahepatic pathobiology of bacterial lipopolysaccharides. Pharmacological Reviews 45: 382–411.
Kubes, P., and D.M. McCafferty. 2000. Nitric oxide and intestinal inflammation. American Journal of Medicine 109: 150–158.
Watson, W.H., Y. Zhao, and R.K. Chawla. 1999. S-adenosylmethionine attenuates the lipopolysaccharide-induced expression of the gene for tumour necrosis factor alpha. Biochemical Journal 342: 21–25.
Itoh, T., H. Obata, S. Murakami, K. Hamada, K. Kangawa, H. Kimura, and N. Nagaya. 2007. Adrenomedullin ameliorates lipopolysaccharide-induced acute lung injury in rats. American Journal of Physiology 293: L446–L452.
Riedemann, N.C., M.E. Murphy, H.S. Murphy, and P.A. Ward. 2003. Regulatory effects of iNOS on acute lung inflammatory responses in mice. American Journal of Pathology 163: 2319–2328.
Chojkier, M., and D.A. Brenner. 1988. Therapeutic strategies for hepatic fibrosis. Hepatology 8: 176–182.
Wu, J., and A. Danielsson. 1994. Inhibition of hepatic fibrogenesis: A review of pharmacologic candidates. Scandinavian Journal of Gastroenterology 29: 385–391.
Cavalli, J.F., F. Tomi, A.F. Bernardini, and J. Casanova. 2004. Combined analysis of the essential oil of Chenopodium ambrosioides by GC, GC-MS and 13C-NMR spectroscopy: Quantitative determination of ascaridole, a heat- sensitive compound. Phytochemical Analysis 15: 275–279.
Khokra, S.L., O. Prakash, S. Jain, K.R. Aneja, and Y. Dhingra. 2008. Essential oil composition and antibacterial studies of Vitex negundo Linn. extracts. Indian Journal of Pharmaceutical Sciences 70: 522–526.
Alma, M.H., A. Mavi, A. Yildirim, M. Digrak, and T. Hirata. 2003. Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biological and Pharmaceutical Bulletin 26: 1725–1729.
Zhong, Weiting, Gefu Chi, and Lanxiang Jiang. 2013. p-Cymene modulates in vitro and in vivo cytokine production by inhibiting MAPK and NF-κB activation. Inflammation 26(3): 529–537.
Yunhe, Fu., Liu Bo, Feng Xiaosheng, et al. 2012. The effect of magnolol on the toll-like receptor 4/nuclear factor kappa B signaling pathway in lipopolysaccharide-induced acute lung injury in mice. European Journal of Pharmacology 689: 255–261.
Rubenfeld, G.D. 2003. Epidemiology of acute lung injury. Critical Care Medicine 31: 276–284.
Nanashima, N., M. Akita, T. Yamada, T. Shimizu, H. Nakano, Y. Fan, et al. 2008. The hairless phenotype of the Hirosaki hairless rat is due to the deletion of an 80-kb genomic DNA containing five basic keratin genes. Journal of Biological Chemistry 283: 16868–16875.
Parsey, M.V., R. Tuder, and E. Abraham. 1998. Neutrophils are major contributors to intraparenchymal lung IL-1β expression after hemorrhage and endotoxemia. The Journal of Immunology 160: 1007–1101.
Shenkar, R., and E. Abraham. 1999. Mechanisms of lung neutrophil activation after hemorrhage or endotoxemia: Roles of reactive oxygen intermediates, NF-κB, and cyclic AMP response element binding protein. The Journal of Immunology 163: 954–962.
Bradley, P.P., D.A. Priebat, R.D. Christensen, and G. Rothstein. 1982. Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. The Journal of Investigative Dermatology 78: 206–209.
Bhatia, M., and S. Moochhala. 2004. Role of inflammatory mediators in the pathophysiology of acute respiratory distress syndrome. Journal of Pathology 202: 145–156.
Cribbs, S.K., M.A. Matthay, and G.S. Martin. 2010. Stem cells in sepsis and acute lung injury. Critical Care Medicine 38: 2379–2385.
Goodman, R.B., J. Pugin, J.S. Lee, and M.A. Matthay. 2003. Cytokine-mediated inflammation in acute lung injury. Cytokine and Growth Factor Reviews 14: 523–535.
Giebelen, I.A., D.J. van Westerloo, G.J. LaRosa, A.F. de Vos, and T. van der Poll. 2007. Local stimulation of alpha7 cholinergic receptors inhibits LPS-induced TNF-alpha release in the mouse lung. Shock 28: 700–703.
Abraham, E. 2000. NF-kappaB activation. Critical Care Medicine 28: 100–104.
The project was supported by Chinese National Project of “Twelfth Five-Year” Plan for Science & Technology Support (2012BAI27B06), the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (no. CZY13016), the Natural Science Foundation of China (81303284) and the Planning of Deeply Participating into the Enterprises for Young Teachers (XD2012060).
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Chen, L., Zhao, L., Zhang, C. et al. Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice. Inflammation 37, 358–364 (2014). https://doi.org/10.1007/s10753-013-9747-3
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