Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Abstract

In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-κB were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-κB protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice.

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Acknowledgments

The project was supported by Chinese National Project of “Twelfth Five-Year” Plan for Science & Technology Support (2012BAI27B06), the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (no. CZY13016), the Natural Science Foundation of China (81303284) and the Planning of Deeply Participating into the Enterprises for Young Teachers (XD2012060).

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Correspondence to Chunfeng Zhang or Zhou Lan.

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Chen, L., Zhao, L., Zhang, C. et al. Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice. Inflammation 37, 358–364 (2014). https://doi.org/10.1007/s10753-013-9747-3

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KEY WORDS

  • p-cymene
  • inflammation
  • LPS-induced acute lung injury
  • NF-κB