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Flos Lonicerae Extracts and Chlorogenic Acid Protect Human Umbilical Vein Endothelial Cells from the Toxic Damage of Perfluorooctane Sulphonate

Inflammation volume 36, pages 767–779 (2013)Cite this article

Abstract

Chlorogenic acid (CGA), one of the most common phenolic acids, is found in many food and traditional Chinese herbs. Various bioactivities of CGA are studied. However, little is known about these properties of Flos Lonicerae extracts, and the difference in the effect between Flos Lonicerae extracts and CGA has not been reported. CGA was identified in Flos Lonicerae extracts by HPLC and determined qualitatively by quadrupole ion trap mass spectrometry. In this study, we evaluated the effect of Flos Lonicerae extracts and CGA on inflammatory-related gene expression, adhesion molecule expression and reactive oxygen species (ROS) production in perfluorooctane sulphonate (PFOS)-treated human umbilical vein endothelial cells (HUVECs). The suppression of transcription of IL-1β, IL-6, COX-2, and P-Selectin genes with Flos Lonicerae extracts was greater than that of CGA in PFOS-treated HUVECs, while the degree of suppression on PFOS-induced expression of NOS3 and ICAM-1 was greater for CGA. Furthermore, the suppressive effect of Flos Lonicerae extracts on adhesion of monocytes onto PFOS-induced HUVECs was greater than that of CGA. In addition, Flos Lonicerae extracts and CGA were highly effective in reducing ROS although their effects were almost comparable. So, Flos Lonicerae extracts exhibited antioxidant activity and CGA was a major contributor to this activity. These results suggest that Flos Lonicerae extracts could be useful to prevent PFOS-mediated inflammatory diseases.

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Abbreviations

CGA:

Chlorogenic acid

PFOS:

Perfluorooctane sulfonate

TCA:

Trichloroacetic acid

SRB:

Sulforhodamine B

DCFH-DA:

2,7-dichlorofluorescein-diacetate

BCECF-AM:

2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester

DMSO:

Dimethyl sulfoxide

PPAR:

Peroxisome proliferator-activated receptors

ER:

Estrogen receptor

ROS:

Reactive oxygen species

VCAM:

Vascular cell adhesion molecule

TNF-α:

Tumor necrosis factor α

ICAM:

Intercellular adhesion molecule

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

HPLC-ESI-MS-MS:

High-performance liquid chromatography-electrospray ionization tandem mass spectrometry

qRT-PCR:

Quantitative real-time reverse-transcription PCR

EDCs:

Endocrine disrupting chemicals

FBS:

Fetal bovine serum

HUVECs:

Human umbilical vein endothelial cells

SD:

Standard deviation

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ACKNOWLEDGEMENTS

This investigation was supported by 100 Talents Program of Chinese Academy of Sciences (KZCX2-YW-BR-18) and the CAS/SAFEA International Partnership Program for Creative Research Teams (KZCX2-YW-T08).

Conflict of Interest Statement

The authors declare that there is no conflict of interest.

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Affiliations

  1. Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, People’s Republic of China

    Yanyan Liao, Sijun Dong, Peng Cai, Liangpo Liu & Heqing Shen

  2. Signaling Molecules Research Group, Biomedical Res. Inst., National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, 305-8566, Japan

    Ryoiti Kiyama

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  1. Yanyan Liao
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  2. Sijun Dong
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  3. Ryoiti Kiyama
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Correspondence to Sijun Dong.

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Liao, Y., Dong, S., Kiyama, R. et al. Flos Lonicerae Extracts and Chlorogenic Acid Protect Human Umbilical Vein Endothelial Cells from the Toxic Damage of Perfluorooctane Sulphonate. Inflammation 36, 767–779 (2013). https://doi.org/10.1007/s10753-013-9603-5

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KEY WORDS

  • adhesion molecule
  • chlorogenic acid
  • Flos Lonicerae extracts
  • HUVECs
  • inflammatory response
  • PFOS
  • ROS