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Cytoprotection of Perfluorocarbon on PMVECs In Vitro

Inflammation volume 36, pages 512–520 (2013)Cite this article

Abstract

Lipopolysaccharide (LPS) can activate endothelial cells and induce inflammatory injury. Toll-like receptor-4 (TLR-4) is integrally involved in LPS signaling and has a requisite role in the activation of nuclear factor (NF)-κB. A number of studies have demonstrated the cytoprotective action of perfluorocarbon (PFC) both in vivo and in vitro, but the exact mechanisms have yet to be elucidated. In this study, we examined in an in vitro model the cytoprotective effect of PFC on LPS-stimulated pulmonary vascular endothelial cells (PMVECs). Intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-α (TNF-α), and interleukin-8 (IL-8) were significantly increased in the LPS-stimulated PMVECs groups. The expression of TLR-4 mRNA and protein in LPS groups was markedly increased. Meanwhile, NF-κB was activated. There were no significant effects of PFC alone on any of the factors studied while the coculture group showed significant downregulation of the secretion of ICAM-1, TNF-α, and IL-8; the expression of TLR-4 mRNA; and the activity of NF-κB. LPS can induce PMVEC inflammatory injury via the activation of TLR-4 and subsequent activation of NF-κB. PFC is able to protect PMVECs from LPS-induced inflammatory injury by blocking the initiation of the LPS signaling pathway.

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Acknowledgments

The authors thank Lu Ying-lin (The Chinese Military Medical Science Institute), Cao Lu, and Cai Fen (Respiratory Laboratory, The Chinese People’s Liberation Army General Hospital) for their technical assistance.

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Authors and Affiliations

  1. Department of Pulmonary Medicine, Chinese People’s Liberation Army General Hospital, Beijing, 100853, People’s Republic of China

    Shufeng Xu, Ping Wang, Zhixin Liang, Xiaowei Zhao & Liangan Chen

  2. Department of Respiratory Medicine, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066000, China

    Shufeng Xu, Kun Wei & Fengsui Liu

  3. Department of Respiratory Medicine, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, China

    Aimin Li

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  1. Shufeng Xu
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  2. Ping Wang
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  3. Kun Wei
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  5. Zhixin Liang
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  6. Xiaowei Zhao
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  7. Aimin Li
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  8. Liangan Chen
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Correspondence to Liangan Chen.

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Shu-feng Xu and Ping Wang contributed equally to this study.

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Xu, S., Wang, P., Wei, K. et al. Cytoprotection of Perfluorocarbon on PMVECs In Vitro . Inflammation 36, 512–520 (2013). https://doi.org/10.1007/s10753-012-9572-0

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  • DOI: https://doi.org/10.1007/s10753-012-9572-0

KEY WORDS

  • perfluorocarbon
  • lipopolysaccharide
  • TLR-4
  • NF-κB
  • pulmonary vascular endothelial cells