Acute Effect of Aloe vera Gel Extract on Experimental Models of Pain

Abstract

The present study was performed to explore the effect of aqueous extract of Aloe vera on behavioural parameters of pain. Pain assessment was performed by the tail-flick and formalin tests. A. vera (100 mg/kg, per oral (p.o.)) produced an insignificant decrease in the pain response in the tail-flick and formalin tests. Moreover, A. vera (200 and 400 mg/kg, p.o.) did not have significant effect on the tail-flick test. However, A. vera (200 and 400 mg/kg, p.o.) significantly decreased the second phase of the formalin-induced pain. Thus, these findings suggest that A. vera exerts its effect by a peripheral mechanism of action rather than central.

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References

  1. 1.

    Das, S., B. Mishra, K. Gill, M.S. Ashraf, A.K. Singh, M. Sinha, S. Sharma, I. Xess, K. Dalal, T.P. Singh, and S. Dey. 2011. Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel. International Journal of Biological Macromolecules 48: 38–43.

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    Rajasekaran, S., K. Sivagnanam, K. Ravi, and S. Subramanian. 2005. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacological Reports 57: 90–96.

    PubMed  Google Scholar 

  3. 3.

    Rajasekaran, S., K. Ravi, K. Sivagnanam, and S. Subramanian. 2006. Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clinical and Experimental Pharmacology and Physiology 33: 232–237.

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Hamman, J.H. 2008. Composition and applications of Aloe vera leaf gel. Molecules 13: 1599–1616.

    PubMed  Article  CAS  Google Scholar 

  5. 5.

    Choi, S.W., B.W. Son, Y.S. Son, Y.I. Park, S.K. Lee, and M.H. Chung. 2001. The wound healing effect of a glycoprotein fraction isolated from Aloe vera. British Journal of Dermatology 145: 535–545.

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Kim, J., I.S. Lee, S. Park, and R. Choue. 2010. Effects of Scutellariae radix and Aloe vera gel extracts on immunoglobulin E and cytokine levels in atopic dermatitis NC/Nga mice. Journal of Ethnopharmacology 132: 529–532.

    PubMed  Article  Google Scholar 

  7. 7.

    Penneys, N.S. 1981. Inhibition of arachidonic acid oxidation by vehicle components. Acta Dermatology and Venereology 62: 59–61.

    Google Scholar 

  8. 8.

    Heggers, J.P., A. Kucukcelebi, D. Listengarten, J. Stabenau, F. Ko, L.D. Broemeling, et al. 1996. Beneficial effect of Aloe on wound healing in an excisional wound model. Journal of Alternative and Complementary Medicine 2: 271–277.

    Article  CAS  Google Scholar 

  9. 9.

    Prabjone, R., D. Thong-Ngam, N. Wisedopas, T. Chatsuwan, and S. Patumraj. 2006. Anti-inflammatory effects of Aloe vera on leukocyte–endothelium interaction in the gastric microcirculation of Helicobacter pylori-infected rats. Clinical Hemorheology and Microcirculation 35: 359–366.

    PubMed  Google Scholar 

  10. 10.

    D'Armour, F.E., and D.L. Smith. 1941. A method for determining loss of pain sensation. Journal of Pharmacology and Experimental Therapeutics 72: 74–79.

    Google Scholar 

  11. 11.

    Abbott, F.V., K.B.J. Franklin, and F. Westbrook. 1995. The formalin test: scoring properties of first and second phases of pain response in rats. Pain 60: 91–102.

    PubMed  Article  CAS  Google Scholar 

  12. 12.

    Park, M.Y., H.J. Kwon, and M.K. Sung. 2011. Dietary aloin, aloesin, or aloe-gel exerts anti-inflammatory activity in a rat colitis model. Life Sciences 88: 486–492.

    PubMed  Article  CAS  Google Scholar 

  13. 13.

    Halder, S., A.K. Mehta, and P.K. Mediratta. 2012. Augmented humoral immune response and decreased cell mediated immunity by Aloe vera in rats. Inflammopharmacology. Epub ahead of print.

  14. 14.

    Park, M.Y., H.J. Kwon, and M.K. Sung. 2009. Evaluation of aloin and aloe-emodin as anti-inflammatory agents in aloe by using murine macrophages. Bioscience, Biotechnology, and Biochemistry 73: 828–832.

    PubMed  Article  CAS  Google Scholar 

  15. 15.

    Vazquez, B., G. Avila, D. Segura, and B. Escalante. 1996. Antiinflammatory activity of extracts from Aloe vera gel. Journal of Ethnopharmacology 55: 69–75.

    PubMed  Article  CAS  Google Scholar 

  16. 16.

    Udupa, A.L., and S.L. Udupa. 1996. Analgesic activity of Aegle marmelos and Aloe vera in mice. Indian Journal of Pharmacology 36: 74.

    Google Scholar 

  17. 17.

    Cai, Y., M. Sun, J. Xing, and H. Corke. 2004. Antioxidant phenolic constituents in roots of Rheum officinale and Rubia cordifolia: structure-radical scavenging activity relationships. Journal of Agricultural and Food Chemistry 52: 7884–7890.

    PubMed  Article  CAS  Google Scholar 

  18. 18.

    Matsuda, H., T. Morikawa, I. Toguchida, J.Y. Park, S. Harima, and M. Yoshikawa. 2001. Antioxidant constituents from rhubarb: structural requirements of stilbenes for the activity and structures of two new anthraquinone glucosides. Bioorganic & Medicinal Chemistry 9: 41–50.

    Article  CAS  Google Scholar 

  19. 19.

    Lee, H.Z., S.L. Hsu, M.C. Liu, and C.H. Wu. 2001. Effects and mechanisms of aloe-emodin on cell death in human lung squamous cell carcinoma. European Journal of Pharmacology 431: 287–295.

    PubMed  Article  CAS  Google Scholar 

  20. 20.

    Kuo, P.L., T.C. Lin, and C.C. Lin. 2002. The antiproliferative activity of aloe-emodin is through p53-dependent and p21-dependent apoptotic pathway in human hepatoma cell lines. Life Sciences 71: 1879–1892.

    PubMed  Article  CAS  Google Scholar 

  21. 21.

    Lin, J.G., G.W. Chen, T.M. Li, S.T. Chouh, T.W. Tan, and J.G. Chung. 2006. Aloe-emodin induces apoptosis in T24 human bladder cancer cells through the p53 dependent apoptotic pathway. Journal of Urology 175: 343–347.

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    Chen, H.C., W.T. Hsieh, W.C. Chang, and J.G. Chung. 2004. Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells. Food and Chemical Toxicology 42: 1251–1257.

    PubMed  Article  CAS  Google Scholar 

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Correspondence to Pramod K. Mediratta.

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Rathor, N., Mehta, A.K., Sharma, A.K. et al. Acute Effect of Aloe vera Gel Extract on Experimental Models of Pain. Inflammation 35, 1900–1903 (2012). https://doi.org/10.1007/s10753-012-9512-z

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KEY WORDS

  • Aloe vera
  • tail-flick test
  • formalin test
  • inflammatory pain