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HSF1 Is a Transcriptional Activator of IL-10 Gene Expression in RAW264.7 Macrophages

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Abstract

The heat shock transcription factor (HSF) is an important transactivator of the heat shock genes. Recent studies have shown that HSF1 acts as a repressor of non-heat shock genes to protect against endotoxemia. In this study, we found that heat shock treatment and HSF1 over-expression augmented the induction of interleukin (IL)-10 mRNA. Computational analysis of the mouse IL-10 promoter region showed that three potential heat shock elements (HSEs) were located at mouse IL-10 gene promoter, among which only the −387/−360 probe formed a complex with HSF1. The lack of binding of the other two HSEs to HSF1 suggested the critical role of the flanking sequences in the binding specificity of HSE to HSF1. Moreover, we showed that HSF1 overexpression transactivated mouse IL-10 gene promoter and this transcriptional activation was inhibited by the mutation of HSE in the −387/−360 region of IL-10 gene promoter using luciferase reporter assay. These findings indicate that HSF1 is a transcriptional activator of anti-inflammatory mediator IL-10 gene in RAW264.7 macrophages.

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ACKNOWLEDGMENTS

This work was supported by funds from the Major National Basic Research Program of China (2007CB512007). Dr. Benjamin and Joel Pieper from University of Utah provided excellent editorial assistance during preparation of this manuscript.

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Authors and Affiliations

  1. Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, 410078, Hunan, People’s Republic of China

    Huali Zhang, Lingli Zhang, Fengxiu Yu, Ying Liu, Qiujuan Liang, Gonghua Deng, Guangwen Chen, Meidong Liu & Xianzhong Xiao

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  1. Huali Zhang
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  2. Lingli Zhang
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Correspondence to Xianzhong Xiao.

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Zhang, H., Zhang, L., Yu, F. et al. HSF1 Is a Transcriptional Activator of IL-10 Gene Expression in RAW264.7 Macrophages. Inflammation 35, 1558–1566 (2012). https://doi.org/10.1007/s10753-012-9471-4

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