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Investigation into the Anti-inflammatory and Antigranuloma Activity of Colchicum luteum Baker in Experimental Models

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Abstract

The present study evaluates the anti-inflammatory and antigranuloma activity of CLHE in experimental models, viz. carrageenan-induced paw edema, subcutaneous cotton pellet implantation-induced granuloma formation, and complete Freund's adjuvant-induced stimulation of peritoneal macrophages in rats. Serum TNF-alpha, IL-6, and IL-1 beta levels were estimated as markers for global effects of inflammation. TNF-R1 protein expression was estimated in stimulated peritoneal macrophages. There was a significant reduction (P < 0.05) of paw edema in the CLHE-treated groups as compared to control. In the cotton pellet-induced granuloma model, there was a significant (P < 0.05) reduction in the dry granuloma weight and serum TNF-alpha, IL-6, and IL-1beta levels in the CLHE-treated group as compared to control. Immunoblot analysis for TNF-R1 also demonstrated a significant reduction in the receptor protein expression on stimulated macrophages. Result of the present study thus demonstrates and validates the antigranuloma activity of CLHE.

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ACKNOWLEDGMENT

The study was funded by an extramural research grant from the Indian Council of Medical Research, New Delhi, India. The funding agency did not have any role in the collection, analysis, interpretation of data, writing of the report, or the decision to submit the paper for publication.

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Authors and Affiliations

  1. Department of Pharmacology, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, 110029, India

    Vinod Nair, Rohit Kumar, Surender Singh & Y. K. Gupta

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  1. Vinod Nair
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  2. Rohit Kumar
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  3. Surender Singh
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  4. Y. K. Gupta
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Correspondence to Surender Singh.

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Nair, V., Kumar, R., Singh, S. et al. Investigation into the Anti-inflammatory and Antigranuloma Activity of Colchicum luteum Baker in Experimental Models. Inflammation 35, 881–888 (2012). https://doi.org/10.1007/s10753-011-9389-2

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