Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis

Abstract

Turmeric (rich in curcuminoids) and ginger (rich in gingerols and shogaols) rhizomes have been widely used as dietary spices and to treat different diseases in Ayurveda/Chinese medicine since antiquity. Here, we compared the anti-inflammatory/anti-oxidant activity of these two plants in rat adjuvant-induced arthritis (AIA). Both plants (at dose 200 mg/kg body weight) significantly suppressed (but with different degrees) the incidence and severity of arthritis by increasing/decreasing the production of anti-inflammatory/pro-inflammatory cytokines, respectively, and activating the anti-oxidant defence system. The anti-arthritic activity of turmeric exceeded that of ginger and indomethacin (a non-steroidal anti-inflammatory drug), especially when the treatment started from the day of arthritis induction. The percentage of disease recovery was 4.6–8.3% and 10.2% more in turmeric compared with ginger and indomethacin (P < 0.05), respectively. The present study proves the anti-inflammatory/anti-oxidant activity of turmeric over ginger and indomethacin, which may have beneficial effects against rheumatoid arthritis onset/progression as shown in AIA rat model.

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References

  1. 1.

    Gabriel, S.E. 2001. The epidemiology of rheumatoid arthritis. Rheumatic Diseases Clinics of North America 27(2): 269–281.

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    Buch, M., and P. Emery. 2002. The aetiology and pathogenesis of rheumatoid arthritis. Hospital Pharmacist 9: 5–10.

    Google Scholar 

  3. 3.

    Carteron, N.L. 2000. Cytokines in rheumatoid arthritis: Trials and tribulations. Molecular Medicine Today 6(8): 315–323.

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Shanahan, J.C., L.W. Moreland, and R.H. Carter. 2003. Upcoming biologic agents for the treatment of rheumatic diseases. Current Opinion in Rheumatology 15(3): 226–236.

    PubMed  Article  CAS  Google Scholar 

  5. 5.

    Szekanecz, Z., M.M. Halloran, M.V. Volin, J.M. Woods, R.M. Strieter, G. Kenneth Haines, S.L. Kunkel, M.D. Burdick, and A.E. Koch. 2000. Temporal expression of inflammatory cytokines and chemokines in rat adjuvant-induced arthritis. Arthritis and Rheumatism 43(6): 1266–1277.

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Goldring, S.R. 2003. Pathogenesis of bone and cartilage destruction in rheumatoid arthritis. Rheumatology (Oxford) 42(2): 11–16.

    Article  Google Scholar 

  7. 7.

    Henrotin, Y.E., P. Bruckner, and J.P. Pujol. 2003. The role of reactive oxygen species in homeostasis and degradation of cartilage. Osteoarthritis and Cartilage 11(10): 747–755.

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Gomes, A., S. Bhattacharya, M. Chakraborty, P. Bhattacharjee, and R. Mishra. 2010. Anti-arthritic activity of Indian monocellate cobra (Naja kaouthia) venom on adjuvant induced arthritis. Toxicon 55(2–3): 670–673.

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Ramprasath, V.R., P. Shanthi, and P. Sachdanandam. 2005. Evaluation of antioxidant effect of Semecarpus anacardium Linn. nut extract on the components of immune system in adjuvant arthritis. Vascul Pharmacol 42(4): 179–186.

    PubMed  Article  CAS  Google Scholar 

  10. 10.

    Quan, L.D., G.M. Thiele, J. Tian, and D. Wang. 2008. The development of novel therapies for rheumatoid arthritis. Expert Opinion on Therapeutic Patents 18(7): 723–738.

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    Payne, R. 2000. Limitations of NSAIDs for pain management: Toxicity or lack of efficacy? Journal of Pain 1(3 Suppl): 14–18.

    PubMed  CAS  Google Scholar 

  12. 12.

    Simon, L.S. 2000. DMARDs in the treatment of rheumatoid arthritis: Current agents and future developments. International Journal of Clinical Practice 54(4): 243–249.

    PubMed  CAS  Google Scholar 

  13. 13.

    Feldmann, M., and R.N. Maini. 2001. Anti-TNF alpha therapy of rheumatoid arthritis: What have we learned? Annual Review of Immunology 19: 163–196.

    PubMed  Article  CAS  Google Scholar 

  14. 14.

    Funk, J.L., J.B. Frye, J.N. Oyarzo, N. Kuscuoglu, J. Wilson, G. McCaffrey, G. Stafford, G. Chen, R.C. Lantz, S.D. Jolad, A.M. Solyom, P.R. Kiela, and B.N. Timmermann. 2006. Efficacy and mechanism of action of turmeric supplements in the treatment of experimental arthritis. Arthritis and Rheumatism 54(11): 3452–3464.

    PubMed  Article  CAS  Google Scholar 

  15. 15.

    Shishodia, S., G. Sethi, and B.B. Aggarwal. 2005. Curcumin: Getting back to the roots. Annals of the New York Academy of Sciences 1056: 206–217.

    PubMed  Article  CAS  Google Scholar 

  16. 16.

    Ali, B.H., G. Blunden, M.O. Tanira, and A. Nemmar. 2008. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research. Food and Chemical Toxicology 46(2): 409–420.

    PubMed  Article  CAS  Google Scholar 

  17. 17.

    Grzanna, R., L. Lindmark, and C.G. Frondoza. 2005. Ginger—an herbal medicinal product with broad anti-inflammatory actions. Journal of Medicinal Food 8(2): 125–132.

    PubMed  Article  CAS  Google Scholar 

  18. 18.

    Phan, P.V., A. Sohrabi, A. Polotsky, D.S. Hungerford, L. Lindmark, and C.G. Frondoza. 2005. Ginger extract components suppress induction of chemokine expression in human synoviocytes. Journal of Alternative and Complementary Medicine 11(1): 149–154.

    Article  Google Scholar 

  19. 19.

    Funk, J.L., J.N. Oyarzo, J.B. Frye, G. Chen, R.C. Lantz, S.D. Jolad, A.M. Solyom, and B.N. Timmermann. 2006. Turmeric extracts containing curcuminoids prevent experimental rheumatoid arthritis. Journal of Natural Products 69(3): 351–355.

    PubMed  Article  CAS  Google Scholar 

  20. 20.

    Ojewole, J.A. 2006. Analgesic, antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats. Phytotherapy Research 20(9): 764–772.

    PubMed  Article  Google Scholar 

  21. 21.

    Narendhirakannan, R.T., S. Subramanian, and M. Kandaswamy. 2007. Anti-inflammatory and lysosomal stability actions of Cleome gynandra L. studied in adjuvant induced arthritic rats. Food and Chemical Toxicology 45(6): 1001–1012.

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry 193(1): 265–275.

    PubMed  CAS  Google Scholar 

  23. 23.

    Ganesan, K., M. Tiwari, C. Balachandran, B.M. Manohar, and R. Puvanakrishnan. 2008. Estrogen and testosterone attenuate extracellular matrix loss in collagen-induced arthritis in rats. Calcified Tissue International 83(5): 354–364.

    PubMed  Article  CAS  Google Scholar 

  24. 24.

    Wroblewski, F., and J.S. Ladue. 1956. Serum glutamic pyruvic transaminase in cardiac with hepatic disease. Proceedings of the Society for Experimental Biology and Medicine 91(4): 569–571.

    PubMed  CAS  Google Scholar 

  25. 25.

    Wenger, C. 1984. Alkaline phosphatase. In Clinical chemistry, ed. A. Kaplan, 1094–1098. Mosby: St Louis.

    Google Scholar 

  26. 26.

    Esterbauer, H., R.J. Schaur, and H. Zollner. 1991. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology & Medicine 11(1): 81–128.

    Article  CAS  Google Scholar 

  27. 27.

    Aebi, H. 1984. Catalase in vitro. Methods in Enzymology 105: 121–126.

    PubMed  Article  CAS  Google Scholar 

  28. 28.

    Flohe, L., and W.A. Gunzler. 1984. Assays of glutathione peroxidase. Methods in Enzymology 105: 114–121.

    PubMed  Article  CAS  Google Scholar 

  29. 29.

    Smith, I.K., T.L. Vierheller, and C.A. Thorne. 1988. Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Analytical Biochemistry 175(2): 408–413.

    PubMed  Article  CAS  Google Scholar 

  30. 30.

    Malstrom, B., L. Andreasson, and B. Reinhammaer. 1975. Copper-containing oxidases and superoxide dismutase. In The enzymes, ed. P.D. Boyer, 533. New York: Academic.

    Google Scholar 

  31. 31.

    Ellman, G.L. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82: 70–77.

    PubMed  Article  CAS  Google Scholar 

  32. 32.

    Turner, J.R., and J.F. Thayer. 2001. Introduction to analysis of variance: Design, analysis and interpretation. Thousand Oaks: Sage Publications.

    Google Scholar 

  33. 33.

    Tamura, T., N. Udagawa, N. Takahashi, C. Miyaura, S. Tanaka, Y. Yamada, Y. Koishihara, Y. Ohsugi, K. Kumaki, T. Taga, T. Kishimoto, and T. Suda. 1993. Soluble interleukin-6 receptor triggers osteoclast formation by interleukin 6. Proceedings of the National Academy of Sciences of the United States of America 90(24): 11924–11928.

    PubMed  Article  CAS  Google Scholar 

  34. 34.

    Hisadome, M., T. Fukuda, H. Sumichika, T. Hanano, and K. Adachi. 2000. A novel anti-rheumatic drug suppresses tumor necrosis factor-alpha and augments interleukin-10 in adjuvant arthritic rats. European Journal of Pharmacology 409(3): 331–335.

    PubMed  Article  CAS  Google Scholar 

  35. 35.

    Yoshihara, Y., H. Nakamura, K. Obata, H. Yamada, T. Hayakawa, K. Fujikawa, and Y. Okada. 2000. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in synovial fluids from patients with rheumatoid arthritis or osteoarthritis. Annals of the Rheumatic Diseases 59(6): 455–461.

    PubMed  Article  CAS  Google Scholar 

  36. 36.

    Bharti, A.C., Y. Takada, and B.B. Aggarwal. 2004. Curcumin (diferuloylmethane) inhibits receptor activator of NF-kappa B ligand-induced NF-kappa B activation in osteoclast precursors and suppresses osteoclastogenesis. Journal of Immunology 172(10): 5940–5947.

    CAS  Google Scholar 

  37. 37.

    Park, C., D.O. Moon, I.W. Choi, B.T. Choi, T.J. Nam, C.H. Rhu, T.K. Kwon, W.H. Lee, G.Y. Kim, and Y.H. Choi. 2007. Curcumin induces apoptosis and inhibits prostaglandin E(2) production in synovial fibroblasts of patients with rheumatoid arthritis. International Journal of Molecular Medicine 20(3): 365–372.

    PubMed  CAS  Google Scholar 

  38. 38.

    Grzanna, R., P. Phan, A. Polotsky, L. Lindmark, and C.G. Frondoza. 2004. Ginger extract inhibits beta-amyloid peptide-induced cytokine and chemokine expression in cultured THP-1 monocytes. Journal of Alternative and Complementary Medicine 10(6): 1009–1013.

    Article  Google Scholar 

  39. 39.

    Hayashida, K., T. Nanki, H. Girschick, S. Yavuz, T. Ochi, and P.E. Lipsky. 2001. Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8. Arthritis Research 3(2): 118–126.

    PubMed  Article  CAS  Google Scholar 

  40. 40.

    Katrib, A., P.P. Tak, J.V. Bertouch, C. Cuello, H.P. McNeil, T.J. Smeets, M.C. Kraan, and P.P. Youssef. 2001. Expression of chemokines and matrix metalloproteinases in early rheumatoid arthritis. Rheumatology (Oxford) 40(9): 988–994.

    Article  CAS  Google Scholar 

  41. 41.

    Gangur, V., F.E. Simons, and K.T. Hayglass. 1998. Human IP-10 selectively promotes dominance of polyclonally activated and environmental antigen-driven IFN-gamma over IL-4 responses. The FASEB Journal 12(9): 705–713.

    PubMed  CAS  Google Scholar 

  42. 42.

    Barber, D.A., and S.R. Harris. 1994. Oxygen free radicals and antioxidants: A review. American Pharmacy NS34(9): 26–35.

    PubMed  CAS  Google Scholar 

  43. 43.

    Hassan, M.Q., R.A. Hadi, Z.S. Al-Rawi, V.A. Padron, and S.J. Stohs. 2001. The glutathione defense system in the pathogenesis of rheumatoid arthritis. Journal of Applied Toxicology 21(1): 69–73.

    PubMed  Article  CAS  Google Scholar 

  44. 44.

    Islamov, B.I., R.M. Balabanova, V.A. Funtikov, Y.V. Gotovskii, and E.E. Meizerov. 2002. Effect of bioresonance therapy on antioxidant system in lymphocytes in patients with rheumatoid arthritis. Bulletin of Experimental Biology and Medicine 134(3): 248–250.

    PubMed  Article  CAS  Google Scholar 

  45. 45.

    Niino-Nanke, Y., H. Akama, M. Hara, and S. Kashiwazaki. 1998. Alkaline phosphatase (ALP) activity in rheumatoid arthritis (RA): Its clinical significance and synthesis of ALP in RA synovium. Ryumachi 38(4): 581–588.

    PubMed  CAS  Google Scholar 

  46. 46.

    Deng, S.L., W.F. Chen, B. Zhou, L. Yang, and Z.L. Liu. 2006. Protective effects of curcumin and its analogues against free radical-induced oxidative haemolysis of human red blood cells. Food Chemistry 98: 112–119.

    Article  CAS  Google Scholar 

  47. 47.

    Ahmed, R.S., V. Seth, and B.D. Banerjee. 2000. Influence of dietary ginger (Zingiber officinales Rosc) on antioxidant defense system in rat: comparison with ascorbic acid. Indian Journal of Experimental Biology 38(6): 604–606.

    PubMed  CAS  Google Scholar 

  48. 48.

    Goldstein, J.L. 2004. Challenges in managing NSAID-associated gastrointestinal tract injury. Digestion 69: 25–33.

    PubMed  Article  CAS  Google Scholar 

  49. 49.

    Charlier, C., and C. Michaux. 2003. Dual inhibition of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) as a new strategy to provide safer non-steroidal anti-inflammatory drugs. European Journal of Medicinal Chemistry 38: 645–659.

    PubMed  Article  CAS  Google Scholar 

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Acknowledgements

The authors thank Mr. Ali A. Al-Kahtani our lab technician, for animal care. This study was supported by the Deanship of Scientific Research, King Faisal University, Kingdom of Saudi Arabia (90066 to G. R.).

Conflicts of Interest

The authors have no potential financial conflicts of interest.

Author contributions

G. R. planned the study (with assistance from M. A. A.-K.), designed and carried out all experiments (except for that in Table 2, which were carried out by W. M. E.-S.), performed the statistical analysis and summarized the results. G. R. drafted the manuscript with assistance from M. A. A.-K. and W. M. E.-S.

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Correspondence to Gamal Ramadan.

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Ramadan, G., Al-Kahtani, M.A. & El-Sayed, W.M. Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis. Inflammation 34, 291–301 (2011). https://doi.org/10.1007/s10753-010-9278-0

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KEY WORDS

  • rat adjuvant-induced arthritis
  • anti-inflammatory/anti-oxidant activity
  • Curcuma longa
  • indomethacin
  • Zingiber officinale