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AV119, a Natural Sugar from Avocado gratissima, Modulates the LPS-Induced Proinflammatory Response in Human Keratinocytes

Inflammation volume 34, pages 568–575 (2011)Cite this article

Abstract

Keratinocytes play an active role in innate immune responses by secreting a variety of cytokines and chemokines. The release of critical proinflammatory cytokines, which are necessary to activate the immune response, is induced by the stimulation of Toll-like receptors (TLRs) by molecules present on pathogenic micro-organisms such as lipopolysaccharide (LPS). AV119, a patented blend of avocado sugars, induced the aggregation of Malassezia furfur, a dimorphic, lipid-dependent yeast that is part of the normal human cutaneous commensal flora and inhibited its penetration into the keratinocytes. In the present study, the anti-inflammatory effects of AV119 were investigated in LPS-induced inflammation of human keratinocytes. In particular, we analysed the modulation of the LPS-induced expression of proinflammatory cytokines and heat shock protein 70 (HSP70) by AV119 and the involvement of TLR-4. Our data show that AV119 is able to modulate significantly the proinflammatory response in human keratinocytes, blocking the NF-kB activation in human keratinocytes.

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ACKNOWLEDGEMENTS

This study was supported by grants from Expanscience Laboratoires, R&D Centre, Epernon, France.

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Affiliations

  1. Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Naples, Italy

    Giovanna Donnarumma, Iole Paoletti, Elisabetta Buommino & Alessandra Fusco

  2. Innovation, Research and Development Department, Laboratoires Expansciences, Epernon, France

    Caroline Baudouin & Philippe Msika

  3. Department of Dermatology, Faculty of Medicine and Surgery, Second University of Naples, Via Pansini, 5, 80138, Naples, Italy

    Adone Baroni

  4. Department of Experimental Medicine, Second University of Naples, Via Costantinopoli, 16, 80100, Naples, Italy

    Maria Antonietta Tufano

Authors
  1. Giovanna Donnarumma
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  2. Iole Paoletti
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  3. Elisabetta Buommino
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  4. Alessandra Fusco
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  5. Caroline Baudouin
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  6. Philippe Msika
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  7. Maria Antonietta Tufano
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  8. Adone Baroni
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Corresponding author

Correspondence to Maria Antonietta Tufano.

Additional information

Giovanna Donnarumma and Iole Paoletti equally contributed to this work.

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Donnarumma, G., Paoletti, I., Buommino, E. et al. AV119, a Natural Sugar from Avocado gratissima, Modulates the LPS-Induced Proinflammatory Response in Human Keratinocytes. Inflammation 34, 568–575 (2011). https://doi.org/10.1007/s10753-010-9264-6

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  • DOI: https://doi.org/10.1007/s10753-010-9264-6

KEY WORDS

  • AV119
  • keratinocytes
  • LPS
  • cytokines
  • TLR-4