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Anti-inflammatory and Immunomodulatory Effects of Baccharis trimera Aqueous Extract on Induced Pleurisy in Rats and Lymphoproliferation In Vitro

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Abstract

Baccharis trimera is a widespread South American plant known as “carqueja”. Medicinal teas prepared from the aerial parts of this plant are used in folk medicine in cases of liver diseases and inflammatory processes. We evaluated the effects of aqueous extract of B. trimera in the experimental inflammatory model of carrageenan-induced pleurisy in rat. The injection of carrageenan into the pleural cavity induces an influx of cells and fluid accumulation with a large number of polymorphonuclear leukocytes and increase of protein levels. The inflammation parameters were attenuated when B. trimera (400 and 800 mg/kg, i.p.) was administrated 30 min before the carrageenan. The immunomodulatory effects were evaluated in vitro on human peripheral blood mononuclear cells. The extract in concentration of 25, 50 and 100 mg/mL presented inhibited the T-lymphocytes proliferation stimulated by phytohemagglutinin, but these extract concentrations also presented cytotoxic effect. These results showed that the aqueous extract of B. trimera has anti-inflammatory effect.

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References

  1. Geetha, S., M. Sai Ram, V. Singh, G. Ilavazhagan, and R. C. Sawhney. 2002. Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides)-and in vitro study. J. Ethnopharmacol. 79:373–378.

    Article  CAS  PubMed  Google Scholar 

  2. Corrêa, M. P. 1984. Dicionário das plantas úteis do Brasil e das exóticas cultivadas. 1Imprensa Nacional, Instituto Brasileiro de Desenvolvimento Florestal, Rio de Janeiro.

    Google Scholar 

  3. Brandão Torres, L. M., M. T. Gamberini, N. F. Roque, M. T. Lima-Landman, C. Souccar, and A. J. Lapa. 2000. Diterpene from Baccharis trimera with a relaxant effect on rat vascular smooth muscle. Phytochemistry. 55:617–619.

    Article  Google Scholar 

  4. De Las Heras, B., K. Slowing, J. Benedi, E. Carretero, T. Ortega, C. Toledo, P. Bermejo, I. Iglesias, M. J. Abad, P. G. Serranillos, P. A. Liso, A. Villar, and X. Chiriboga. 1998. Anti-inflammatory and antioxidant activity of plants used in traditional medicine in Ecuador. J. Ethnopharmacol. 61:161–166.

    Article  Google Scholar 

  5. Soicke, H., and A. Leng-Peschlow. 1987. Characterisation of flavonoids from Baccharis trimera and their antihepatotoxic properties. Planta Med. 53(1):37–39.

    Article  CAS  PubMed  Google Scholar 

  6. Gené, R. M., E. Marin, and T. Adzet. 1992. Anti-inflammatory effect of aqueous extracts of three species of the genus Baccharis. Planta Med. 58:37–39.

    Article  Google Scholar 

  7. Gené, R. M., C. Cartañá, T. Adzet, E. Marin, T. Parella, and S. Cañigueral. 1996. Anti-inflammatory and analgesic activity of Baccharis trimera: identification of its active constituents. Planta Med. 62:232–235.

    Article  PubMed  Google Scholar 

  8. Srinivasan, K., S. Muruganandan, J. Lal, S. Chandra, S. K. Tandan, and V. Ravi Prakash. 2001. Evaluation of anti-inflammatory activity of Pongamia pinnata leaves in rats. J Ethnopharmacol. 78:151–157.

    Article  CAS  PubMed  Google Scholar 

  9. Saleh, T. S. F., J. B. Calixto, and Y. S. Medeiros. 1999. Effects of anti-inflammatory drugs upon nitrate and myeloperoxidase levels in the mouse pleurisy induced by carregeenan. Peptides. 20:949–956.

    Article  CAS  PubMed  Google Scholar 

  10. Alves Filho, J. C. F., R. C. V. Santos, T. A. Castaman, and J. R. Oliveira. 2004. Anti-inflammatory effects of fructose-1,6-bisphosphate on carrageenan-induced pleurisy in rat. Pharmacol. Res. 49:245–248.

    Article  CAS  PubMed  Google Scholar 

  11. Lo, T. N., A. P. Almeida, and M. A. Braven. 1982. Dextran and carrageenan evoke different inflammatory responses in rat with respect to composition of infiltrates and effect of indomethacin. J. Pharmacol. Exp. Ther. 221:261–267.

    CAS  PubMed  Google Scholar 

  12. Saleh, T. S., J. B. Calixto, and I. S. Medeiros. 1996. Anti-inflammatory effects of theophylline, cromolyn and salbutamol in a murine model of pleurisy. Br. J. Pharmacol. 118:811–819.

    CAS  PubMed  Google Scholar 

  13. Lopes, R. P., A. Lunardelli, T. Preissler, C. E. Leite, J. C. F. Alves-Filho, F. B. Nunes, J. R. Oliveira, and M. E. Bauer. 2006. The effects of fructose-1,6-bisphosphate and dexamethasone on acute inflammation and T-cell proliferation. Inflamm. Res. 55:354–358.

    Article  CAS  PubMed  Google Scholar 

  14. Severino, D. E., R. L. Pereira, I. Knysak, D. M. Cândido, and F. H. Kwasniewski. 2009. Edematogenic activity of scorpion venoms from the Buthidae family and the role of platelet-activating factor and nitric oxide in paw edema induced by Tityus venoms. Inflammation. 32(1):57–64.

    Article  CAS  PubMed  Google Scholar 

  15. Souza-Fagundes, E. M., G. Gazzinelli, G. G. Parreira, O. A. Martins-Filho, G. P. Amarante-Mendes, R. Correa-Oliveira, and C. L. Zani. 2003. In vitro activity of labdane diterpene from Alomia myriadenia (Asteraceae): immunosuppression via induction of apoptosis in monocytes. Int. Immunopharmacol. 3:383–392.

    Article  CAS  PubMed  Google Scholar 

  16. Dutta, R. C. 2002. Peptide immunomodulators versus infection; an analysis. Immunol. Lett. 83(3):153–161.

    Article  CAS  PubMed  Google Scholar 

  17. Leite, C. E., A. Lunardelli, T. A. Castaman, E. L. Paul, and J. R. Oliveira. 2007. Extrato aquoso de Baccharis trimera (Asteraceae) diminui a inflamação e o dano celular em pleurisia induzida por veneno de Dirphia sp. (Saturniidae). Rev. Bras. Anál. Clín. 39:29–32.

    Google Scholar 

  18. De Oliveira, S. Q., G. Barbon, S. Bordignon, and G. Gosmann. 2006. Diferentation of south Brazilian Baccharis species by TLC. J. Liq. Chromatogr. Relat. Technol. 29:2603–2609.

    Article  Google Scholar 

  19. Wagner, H. 1983. Drogenanalyse: Dunnschichtchromatographishe Analyse von Arzneidrogen. Springer, Berlin, pp. 291–293.

    Google Scholar 

  20. Lunardelli, A., C. E. Leite, M. G. S. Pires, and J. R. Oliveira. 2006. Extract of the bristles of Dirphia sp. increases nitric oxide in a rat pleurisy model. Inflamm. Res. 55:129–135.

    Article  CAS  PubMed  Google Scholar 

  21. Nunes, F. B., C. M. Graziottin, J. C. F. Alves-Filho, A. Lunardelli, E. Caberlon, A. Peres, and J. R. Oliveira. 2003. Immunomodulatory effect of fructose-1,6-bisphosphate on T-lymphocytes. Int. Immunopharmacol. 3:267–272.

    Article  Google Scholar 

  22. Suffredini, A. F., G. Fantuzzi, R. Badolato, J. J. Oppnheim, and N. P. O’Grady. 1999. New insights into the biology of the acute phase response. J. Clin. Immunol. 19:203–214.

    Article  CAS  PubMed  Google Scholar 

  23. Amirghofran, Z., M. Azadbakht, and M. H. Karimi. 2000. Evaluation of the immunomodulatory effects of five herbal plants. J. Ethnopharmacol. 72:167–172.

    Article  CAS  PubMed  Google Scholar 

  24. Mongelli, E., C. Desmarchelier, T. J. Rodrigues, J. Coussio, and G. Ciccia. 1997. In vitro antioxidant and cytotoxic activity of extracts of Baccharis coridifolia DC. J. Ethnopharmacol. 58:157–163.

    Article  CAS  PubMed  Google Scholar 

  25. Boschi, E. S., C. E. Leite, V. C. Saciura, E. Caberlon, A. Lunardelli, S. Bitencourt, D. A. S. Melo, and J. R. Oliveira. 2008. Anti-inflammatory effects of low-level laser therapy (660 nm) in the early phase in carrageenan-induced pleurisy in rat. Lasers Surg. Med. 40:500–508.

    Article  PubMed  Google Scholar 

  26. Aimbire, F., A. P. L. Oliveira, R. Albertini, J. C. Corrêa, C. B. L. Campos, J. P. Lyon, J. A. Silva Jr., and M. S. Costa. 2008. Low level laser therapy (LLLT) decreases pulmonary microvascular leakage, neutrophil influx and IL-1β levels in airway and lung from rat subjected to LPS-induced inflammation. Inflammation. 31(3):189–197.

    Article  CAS  PubMed  Google Scholar 

  27. Spiller, F., M. K. Alves, S. M. Vieira, T. A. Carvalho, C. E. Leite, A. Lunardelli, J. A. T. Poloni, F. Q. Cunha, and J. R. Oliveira. 2008. Anti-inflammatory effects of red pepper (Capsicum baccatum) on carrageenan- and antigen-induced inflammation. J. Pharm. Pharmacol. 60:473–478.

    Article  CAS  PubMed  Google Scholar 

  28. Hodek, P., P. Trefil, and M. Stiborová. 2002. Flavonoids-potent and versatile biologically active componds interacting with cytochromes P450. Chem.-Biol. Interact. 139:1–21.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, prédio 12, bloco C, sala 263, Porto Alegre, Rio Grande do Sul, Brasil, 90.619–900

    Elisiane Lozza Paul, Adroaldo Lunardelli, Eduardo Caberlon, Roberto Christ Vianna Santos, Vanderlei Biolchi, Carolina Maria Alves Bastos, Karla Barbosa Moreira, Fernanda Bordignon Nunes & Jarbas Rodrigues de Oliveira

  2. Laboratório de Fitoquímica, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil

    Cristiane Bernardes de Oliveira & Grace Gosmann

Authors
  1. Elisiane Lozza Paul
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  2. Adroaldo Lunardelli
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  3. Eduardo Caberlon
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  4. Cristiane Bernardes de Oliveira
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  6. Vanderlei Biolchi
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  7. Carolina Maria Alves Bastos
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  9. Fernanda Bordignon Nunes
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  11. Jarbas Rodrigues de Oliveira
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Correspondence to Jarbas Rodrigues de Oliveira.

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Paul, E.L., Lunardelli, A., Caberlon, E. et al. Anti-inflammatory and Immunomodulatory Effects of Baccharis trimera Aqueous Extract on Induced Pleurisy in Rats and Lymphoproliferation In Vitro . Inflammation 32, 419–425 (2009). https://doi.org/10.1007/s10753-009-9151-1

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