Skip to main content
SpringerLink
Log in

Procaterol Inhibits Lung Fibroblast Migration

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Fibroblasts are important cells that are involved in modulation of fibrosis after injuries. In some uncontrollable inflammatory processes, excess fibroblasts migrate around the small airway. The pathogenesis of chronic obstructive pulmonary disease is related to fibrosis around the small airways. The aim of the current study was to investigate the effect of procaterol, a second-generation beta (2)-agonist, on migration of human fetal lung fibroblasts (HFL-1) induced by human plasma fibronectin (HFn). Using the blindwell chamber technique, 10−8 M procaterol inhibited migration of HFL-1 (control, 100%; 10−8 M, 73.2 ± 4.9%; n = 6, p < 0.05). The inhibitory effect of procaterol was concentration-dependent. Although a beta 2-receptor inhibitor, ICI 181551, blocked the inhibitory effect of procaterol, a beta 1-receptor inhibitor, atenolol, did not. Because a cyclic AMP-dependent protein kinase (PKA) inhibitor, KT5720, blocked the effect of procaterol, the cyclic AMP–PKA pathway may be involved in the migration inhibitory process. Procaterol, which is prescribed mainly for treatment of bronchial asthma, might be a useful drug for inhibiting lung fibrosis following injuries to the lung.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Rennard, S. I., M. C. Jaurand, J. Bignon, et al. 1984. Role of pleural mesothelial cells in the production of the submesothelial connective tissue matrix of lung. Am. Rev. Respir. Dis. 130:267–274.

    CAS  PubMed  Google Scholar 

  2. Mirza, Z. N., M. Kato, H. Kimura, et al. 2002. Fenoterol inhibits superoxide anion generation by human polymorphonuclear leukocytes via beta-adrenoceptor-dependent and -independent mechanisms. Ann. Allergy Asthma Immunol. 88:494–500.

    Article  CAS  PubMed  Google Scholar 

  3. Fujisawa, T., Y. Kato, A. Terada, et al. 2002. Synergistic effect of theophylline and procaterol on interleukin-5-induced degranulation from human eosinophils. J. Asthma 39:21–27.

    Article  CAS  PubMed  Google Scholar 

  4. Barnes, P. J. 1999. Effect of beta-agonists on inflammatory cells. J. Allergy Clin. Immunol. 104:S10–S17.

    Article  CAS  PubMed  Google Scholar 

  5. Johnson, M., and S. Rennard. 2001. Alternative mechanisms for long-acting beta(2)-adrenergic agonists in COPD. Chest 120:258–270.

    Article  CAS  PubMed  Google Scholar 

  6. Boyden, S. 1962. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J. Exp. Med. 115:453–466.

    Article  CAS  PubMed  Google Scholar 

  7. Kohyama, T., R. F. Ertl, V. Valenti, et al. 2001. Prostaglandin E(2) inhibits fibroblast chemotaxis. Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1257–L1263.

    CAS  PubMed  Google Scholar 

  8. Kohyama, T., X. D. Liu, F. Q. Wen, et al. 2002. Prostaglandin D2 inhibits fibroblast migration. Eur. Respir. J. 19:684–689.

    Article  CAS  PubMed  Google Scholar 

  9. Hunninghake, W. C., K. C. Garret, H. B. Richerson, et al. 1984. Pathogenesis of the granulomatous lung disease (state of art). Am. Rev. Respir. Dis. 130:476–496.

    CAS  PubMed  Google Scholar 

  10. Kohyama, T., X. Liu, F. Q. Wen, et al. 2002. PDE4 inhibitors attenuate fibroblast chemotaxis and contraction of native collagen gels. Am. J. Respir. Cell Mol. Biol. 26:694–701.

    CAS  PubMed  Google Scholar 

  11. Clark, J. G., K. M. Kostal, and B. A. Marino. 1982. Modulation of collagen production following bleomycin-induced pulmonary fibrosis in hamsters. Presence of a factor in lung that increases fibroblast prostaglandin E2 and cAMP and suppresses fibroblast proliferation and collagen production. J.Biol. Chem. 257:8098–8105.

    CAS  PubMed  Google Scholar 

  12. Zhu, Y., C. M. Skold, X. Liu, et al. 2001. Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture. Respir. Res. 2:295–299.

    Article  CAS  PubMed  Google Scholar 

  13. Mio, T., Y. Adachi, S. Carnevali, et al. 1996. Beta-adrenergic agonists attenuate fibroblast-mediated contraction of released collagen gels. Am. J. Physiol. 270:L829–L835.

    CAS  PubMed  Google Scholar 

  14. Naito, K., T. Nagao, M. Otsuka, et al. 1985. Studies on the affinity and selectivity of denopamine (TA-064), a new cardiotonic agent, for beta-adrenergic receptors. Jpn. J. Pharmacol. 38:235–241.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported in part by grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports, Culture and Technology of Japan, a grant to the Respiratory Failure Research Group from the Ministry of Health, Labour and Welfare, Japan, and grants-in-aid for Comprehensive Research on Aging and Health from the Ministry of Health, Labour and Welfare, Japan. The authors thank Ms. Makiko Kase for her excellent technical assistance.

Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Tadashi Kohyama, Yasuhiro Yamauchi, Susumu Itakura, Sumiko Kamitani, Masashi Desaki, Shin Kawasaki & Takahide Nagase

  2. Fourth Department of Internal Medicine, School of Medicine, Teikyo University, Kawasaki, Japan

    Hajime Takizawa

  3. School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, Japan

    Tadashi Kohyama

Authors
  1. Tadashi Kohyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuhiro Yamauchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hajime Takizawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Susumu Itakura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sumiko Kamitani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Masashi Desaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shin Kawasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Takahide Nagase
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tadashi Kohyama.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kohyama, T., Yamauchi, Y., Takizawa, H. et al. Procaterol Inhibits Lung Fibroblast Migration. Inflammation 32, 387–392 (2009). https://doi.org/10.1007/s10753-009-9147-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-009-9147-x

KEY WORDS

Search

Navigation