Inflammation

, 31:215 | Cite as

Hepatoprotective Effect of Infliximab, an Anti-TNF-α Agent, on Carbon Tetrachloride-Induced Hepatic Fibrosis

  • Ibrahim Halil Bahcecioglu
  • Suleyman Serdar Koca
  • Orhan Kursat Poyrazoglu
  • Mehmet Yalniz
  • Ibrahim Hanifi Ozercan
  • Bilal Ustundag
  • Kazim Sahin
  • Adile Ferda Dagli
  • Ahmet Isik
Article

Abstract

To assess the effect of infliximab, an anti-tumor necrosis factor (TNF)-α agent, on the carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Rats were randomized into three groups (n = 9). The control group received only intraperitoneal (i.p.) olive oil. Hepatic fibrosis was induced by repeated i.p. injections of 1.5 ml/kg CCl4 (1:3 mixture with olive oil) for 5 weeks in the remaining two groups which were also injected subcutaneous saline or 2 mg/kg infliximab. Infliximab reduced the levels of aspartate aminotransferase and alanine aminotransferase (p < 0.05 for both). The scores of hepatic necrosis, inflammation and fibrosis, and expression of α-smooth muscle actin were lower in the infliximab-treated group than the CCI4-treated group (p < 0.01, p < 0.001, p < 0.01, p < 0.001, respectively). However, there was no significant difference in terms of liver tissue and plasma malondialdehyde, and serum TNF-α levels, while infliximab relatively reduced the level of transforming growth factor-β1 (373.0 ± 153.1 vs. 280.8 ± 127.1 pg/ml). Treatment with infliximab attenuated the necro-inflammation and fibrogenesis in the CCI4-induced hepatic fibrosis, and thus it might be effective as a therapeutic anti-fibrotic agent.

KEY WORDS

carbon tetrachloride hepatic fibrosis infliximab 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Ibrahim Halil Bahcecioglu
    • 1
  • Suleyman Serdar Koca
    • 2
    • 6
  • Orhan Kursat Poyrazoglu
    • 1
  • Mehmet Yalniz
    • 1
  • Ibrahim Hanifi Ozercan
    • 3
  • Bilal Ustundag
    • 4
  • Kazim Sahin
    • 5
  • Adile Ferda Dagli
    • 3
  • Ahmet Isik
    • 2
  1. 1.Department of Gastroenterology, Faculty of MedicineFırat UniversityElazigTurkey
  2. 2.Department of Internal Medicine and Rheumatology, Faculty of MedicineFırat UniversityElazigTurkey
  3. 3.Department of Pathology, Faculty of MedicineFırat UniversityElazigTurkey
  4. 4.Department of Biochemistry, Faculty of MedicineFırat UniversityElazigTurkey
  5. 5.Department of Animal Nutrition, Faculty of VeterinaryFırat UniversityElazigTurkey
  6. 6.Tıp Fakultesi, RomatolojiFırat universitesiElazigTurkey

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