Effects of Resveratrol in Inflammatory Arthritis

Abstract

Summary:

Nuclear factor kappa B (NF-κB), is a pivotal transcription factor involved in the activation of the TNF-α and IL-1β genes. Activation of NF-κB in synovial cells is a feature seen in arthritis patients. Resveratrol, a polyphenolic, natural phytoalexin found with particularly high levels in grape skin and red wine is potent and specific inhibitor of TNF-α and IL-1β induced NF-κB activation. We aimed to determine the in vivo effects of intra-articular injections of resveratrol on cartilage and synovium in an experimental rabbit inflammatory arthritis model.

Materials and methods:

Arthritis was induced by intra-articular injection of three times of 50 μg lipopolysaccharide (LPS) at day 0, 4 and 8 at 4-day intervals into the knee joints of rabbits. To the test group, 10 μMol/kg resveratrol in the DMSO was injected in the knees at day 0 and then it was continued once daily for 2 weeks. To the control group the same time and amount of DMSO was injected the knees of rabbits. All rabbits were killed 1 week after the last injection and cartilage tissue and synovium were evaluated with semiquantitative scoring histologically.

Results:

According to control group in the resveratrol group, significantly decreased cartilage destruction was determined by H&E staining (p = 0.04). Loss of matrix proteoglycan content in the cartilage was much lower, as determined by safranin O staining (p = 0.03). We also observed marked synovial inflammation after intra-articular injection to control knees, but not in the resveratrol treated group knees (p = 0.01).

Conclusion:

This study suggests that intra-articular injection of resveratrol may protect cartilage against the development of experimentally induced IA.

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Correspondence to N. Elmali.

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Elmali, N., Baysal, O., Harma, A. et al. Effects of Resveratrol in Inflammatory Arthritis. Inflammation 30, 1–6 (2007). https://doi.org/10.1007/s10753-006-9012-0

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Key words

  • resveratrol
  • cartilage
  • synovium
  • inflammatory arthritis
  • NF-κB