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Measurement of electric field gradient at 117In on the Cu-site in mavicyanin by perturbed angular correlation of γ-rays

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Abstract

The structure around the metal site of mavicyanin, a protein molecule with a copper site, was investigated in solution by using time-differential perturbed angular correlation of 117In. The electric field gradient (EFG) of the metal site was deduced from the measurement. It demonstrated that the site in a mutant-type mavicyanin, Thr15Ala-Mav, gives an EFG different from that in the wild-type mavicyanin does. The pH dependence of the EFG was also observed for both proteins.

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Authors and Affiliations

  1. Faculty of Science and Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Ishikawa, 920-1192, Japan

    A. Yokoyama, T. Hashimoto, K. Ihara, H. Kikunaga, N. Kinoshita, I. Yamazaki, K. Kataoka, M. Yanase & M. Takata

  2. Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka, 590-0494, Japan

    Y. Murakami, K. Takamiya, M. Tanigaki & Y. Ohkubo

Authors
  1. A. Yokoyama
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  2. T. Hashimoto
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  3. K. Ihara
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  4. H. Kikunaga
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  5. N. Kinoshita
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  6. I. Yamazaki
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  7. K. Kataoka
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  8. M. Yanase
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  9. M. Takata
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  10. Y. Murakami
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  11. K. Takamiya
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  12. M. Tanigaki
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  13. Y. Ohkubo
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Corresponding author

Correspondence to A. Yokoyama.

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Yokoyama, A., Hashimoto, T., Ihara, K. et al. Measurement of electric field gradient at 117In on the Cu-site in mavicyanin by perturbed angular correlation of γ-rays. Hyperfine Interact 181, 69–73 (2008). https://doi.org/10.1007/s10751-008-9691-z

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  • DOI: https://doi.org/10.1007/s10751-008-9691-z

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