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Geometric morphometrics shows a close relationship between the shape features, position on thalli, and CaCO3 content of segments in Halimeda tuna (Bryopsidales, Ulvophyceae)

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Abstract

Calcifying green algae of the genus Halimeda J.V. Lamouroux are typical for the modular thalli composed of serial segments. Their CaCO3 content gradually increases with age due to calcification, the intensity of which is largely linked to photosynthesis. The dynamics of segment phenotypic plasticity at different scales and its relation to CaCO3 content is not well known. We investigated the populations of Halimeda tuna in the upper sublittoral of four regions on the Adriatic Sea coast. Using geometric morphometrics, we explored the patterns of segment shape plasticity, their relationships with the spatial factors and CaCO3 content. The results showed that segment position on thalli was the main determinant of their shape features. This effect was considerably more prominent than the differences among plants, populations, or regions. Likewise, the segment shape proved to be a significant predictor of their CaCO3 content. Segments with inversely conical shapes, typical for the lower parts of branches, contained significantly less CaCO3 than the reniform and oval segments that probably contribute most to the overall carbonate budget of the Mediterranean Halimeda draperies.

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Data availability

Primary data used for the analyses are available online at: http://doi.org/10.5281/zenodo.5504135

Code availability

The R scripts used for the analyses are available online at: http://doi.org/10.5281/zenodo.5504135

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Acknowledgements

The authors wish to thank the Ministry of the Sea, Transport, and Infrastructure of the Republic of Croatia for the field research permit no. 6422/2019/JLJ and Prof. Dr. Zrinka Ljubešić for her kind assistance in arranging the research permission. The study was supported by the institutional grant of Charles University ‘Progres Q43’.

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The study was supported by the institutional grant of Charles University ‘Progres Q43’.

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  1. Department of Botany, Faculty of Science, Charles University Prague, Prague, Czech Republic

    Jiri Neustupa & Yvonne Nemcova

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  1. Jiri Neustupa
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Correspondence to Jiri Neustupa.

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Neustupa, J., Nemcova, Y. Geometric morphometrics shows a close relationship between the shape features, position on thalli, and CaCO3 content of segments in Halimeda tuna (Bryopsidales, Ulvophyceae). Hydrobiologia 849, 2581–2594 (2022). https://doi.org/10.1007/s10750-022-04876-y

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