Abstract
Several Alpine streams are currently facing recurrent summer drying events with detrimental consequences on stream detritivores, i.e., shredders, due to negative effects via changes the organic matter (CPOM) availability. We examined the ecological requirements of three phylogenetically related shredder genera belonging to the family of Nemouridae (Plecoptera), namely Nemoura, Protonemura and Amphinemura, in 14 Alpine streams recently facing recurrent summer flow intermittency events. We evaluated the overlap among their ecological niches measured in terms of hydraulic stress, substrate composition, changes in CPOM availability and competition with other shredder taxa (i.e., presence of individuals of other shredders) and we examined potential changes in their ecological niches between permanent and intermittent sites. The ecological niches of Protonemura and Amphinemura overlap broadly, but not with Nemoura, suggesting only partial potential competition. The reduced CPOM availability decreased the individual abundance of the three genera in intermittent sites, where they consistently preferred microhabitats with high CPOM availability and low competition with other shredder taxa, possibly due to food limitation. Overall, our results emphasize how the negative effect of flow intermittency on shredders in Alpine streams is mainly due to the decrease in CPOM availability, with consequent potential bottom up effects on stream ecosystem functionality.
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Introduction
Mountain low order lotic systems have always been characterized by highly predictable natural hydrological and geomorphological dynamics, with an increase in water flow and fine sediments in summer, during snow melting, and a minimum discharge in winter (McGregor et al., 1995). The recurrent occurrence of flow intermittency is currently becoming one of the most dramatic threats to mountain streams (Fenoglio et al., 2010; Brighenti et al., 2019), which are changing from perennial to temporary systems. These newly temporary streams are characterized with recurrent non-flow events, occurring in summer, followed by rewetting phases in late autumn (Fenoglio et al., 2010) due to the interactive effects of both climate change and anthropogenic disturbance (Belmar et al., 2019; Bruno et al., 2019). These recurrent drying events are expected to alter the distribution of lotic biota by influencing physical conditions and distribution of trophic resources (e.g., Calapez et al., 2014; Elias et al., 2015; Milner et al., 2017; Doretto et al., 2018; Falasco et al., 2018; Piano et al., 2019a; Doretto et al., 2020a).
Stream detritivores, which feed on fragments of leaf litter and other plant detritus (i.e., shredders, sensu Merritt et al., 2017), represent a key component in the lotic food web of mountain streams (Boyero et al., 2012). In fact, small, upland, snow melt driven streams in temperate regions are mainly heterotrophic ecosystems, as most of the energetic support is allochthonous (Vannote et al., 1980) and originate from terrestrial plant organic matter, such as dead leaves introduced during autumn abscission in forested areas (e.g., Petersen & Cummins, 1974; Webster & Meyer, 1997; Merritt et al., 2017) or grass fragments in arctic-alpine areas (Fenoglio et al., 2014; Taylor & Andrushchenko, 2014). In particular, detritivores have evolved to take advantage of pulsed organic matter inputs (Benstead & Huryn, 2011), having their life cycles synchronized with the autumnal litter fall (Fenoglio et al., 2005). Early instars take advantage of the organic matter that enters the streams and feed on CPOM until individuals are ready to emerge as winged adults in late spring/early summer (Cummins et al., 1989; Bo et al., 2013; Ferreira et al., 2013). This enables shredder biomass and body size to increase throughout the winter and to reach a maximum in early spring, just before the adult insects emerge from the water before the expected increase in flow due to snow melt (González & Graça, 2003; Fenoglio et al., 2005; Tierno de Figueroa et al., 2009; Bo et al., 2013).
Therefore, as flow intermittency events occur in summer, they are expected to impact shredders during the oviposition and early instars, greatly reducing their survival and abundance, but also because recurrent drying events significantly alter organic matter processing, as already observed in Mediterranean streams (e.g., Abril et al., 2016). For instance, surface flow disappearance usually reduces the decomposition rate especially in dry streambed sediments, where the activity of Ingoldian fungi, bacteria and invertebrates are inhibited by emersion (Corti et al., 2011; Receveur et al., 2020). In addition, lower flow velocity reduces the removal of fine sediments, with consequent high fine sediment deposition, which can alter the quality and quantity of resources, in terms of both reduced in-stream production, due to the abrasion of autotrophic biofilms (Henley et al., 2000; Bona et al., 2016), and allochthonous coarse organic matter availability through burial (Doretto et al., 2016). On top of that, such dramatic consequences on organic matter availability and decomposition may persist even after several months following flow resuming, because of the so-called “drying memory” (Datry et al., 2011; Pinna et al., 2016), thus affecting also the late instars of shredders (Piano et al., 2019a). Physical alterations and consequent changes in food resources induced by flow intermittency thus represent strong environmental filters that may influence the ecological niche of shredders. Although some studies investigated the effect of flow intermittency on the diversity of this trophic group in Alpine streams (e.g., Fenoglio et al., 2007; Doretto et al., 2018; Piano et al., 2019a), little is known about how these events may alter the ecological parameters that determine the niches of shredder taxa.
Here, we investigated the influence of flow intermittency, CPOM availability, hydromorphological parameters and competition on the distribution and the ecological niche of three phylogenetically related shredder genera, namely Nemoura, Protonemura and Amphinemura, belonging to the Nemouridae (Plecoptera) family. We focused our attention on these genera as they are expected to be particularly sensitive to flow intermittency due to their life-history and ecological traits, as they are medium-sized, monovoltine crawlers, with aquatic respiration, preferring fast flowing waters and coarse substrates (Usseglio-Polatera et al., 2000; Tachet et al., 2010). We conducted our study in fourteen streams in Italian SW-Alps experiencing summer flow intermittency since 2011, where we evaluated the distribution of our focal genera during base flow conditions in April 2017, when shredder larvae reach their maximum density and size. This work is part of the research project NOACQUA dedicated to the investigation of the effect of flow intermittency on the biodiversity and ecosystem processes in mountain Italian streams, which has already published data on other macroinvertebrate groups (i.e., scrapers, see Piano et al., 2019b). We hypothesized that: (i) the ecological niches of the three examined genera overlap, thus suggesting possible exploitative competition; (ii) flow intermittency negatively affects their abundances; and (iii) recurrent drying events change their ecological niches.
Materials and methods
Sampling design
This study was conducted in fourteen low order streams located in the hydroecoregion of SW Alps (HER 4, Piemonte, NW Italy; Wasson et al., 2007), characterized by similar geology, climate and altitude. Study streams were selected based on our experience and available historical data on their hydrology (ARPA – local Environmental Protection Agency). In each stream, we selected two sampling reaches differing in their hydrological regime: (i) a control section, with permanent water during the whole year (hereinafter PS); (ii) an intermittent section, which experiences drying events during summer (hereinafter IS) caused by factors acting at both global (i.e., climate change) and local (i.e., water abstraction) scales. In particular, ISs have already been facing summer drying lasting on average two months since 2011, with the riverbed almost completely dry for several kilometers (ARPA, 2013). We selected PSs within 10 km upstream of the ISs to reduce environmental variation between the two reaches. At the same time, due to the low drift propensity of Nemouridae (e.g., Bruno et al., 2013), permanent and intermittent sites within the same stream may be considered independent sampling units. Sampling site elevation was on average 489 m a.s.l., ranging from 307 m and 656 m, and permanent and intermittent reaches within the same stream differed on average of 70.2 m in their elevation (min = 19 m; max = 155 m). We performed our sampling campaign in April 2017, before summer snow melt (6 months after the drying period), under moderate flow (Qmean = 3.98 ± 4.56 m3/s) occurring in both sections, which allowed us to sample shredders at their latest instars and in the period of their maximum biomass. Water flow in ISs had resumed in November 2016 after a heavy rain event, ending the dry period (Hydrological bulletins, www.arpa.piemonte.it).
In each reach we selected seven independent sampling patches within 30 m long stream section of longitudinal distribution, overall spaced at least 5 m apart, which were randomly selected to cover different habitat conditions of flow velocity, water depth and substrate composition (7 samples × 2 reaches × 14 streams = 196 samples). In each patch, which consisted of a surface of 0.062 m2, i.e., the area of the Surber sampler, we measured flow velocity (0.05 m from the bottom) and water depth with a current meter (Hydro-bios Kiel) and we visually estimated percentages of different substratum sizes measured with a gravelometer following the classification of Wentworth, namely boulders (> 256 mm), cobbles (64–256 mm), gravel (2–64 mm) and fine sediment (< 2 mm). One sample was collected in each sampling patch, by using a Surber sampler (250 μm mesh size; 0.062 m2 area) and we collected both the retained CPOM and macroinvertebrates (Doretto et al., 2020b). Collected samples were preserved in plastic jars with 75% ethanol. In the laboratory, CPOM was washed through a 250 μm mesh sieve to eliminate mineral detritus and subsequently separated from macroinvertebrates. The material was subsequently air dried for 24 h, oven dried (105°C) for 24 h, and then weighed with an electronic balance (accuracy 0.001 g). The CPOM mass, expressed as mg/m2, was then considered a proxy of CPOM availability in subsequent analyses. In the laboratory, all benthic invertebrates were identified according to Campaioli et al. (1994, 1999) to the family or genus level and counted. Only data referred to shredder taxa were considered for further analysis (see Table S1 and Fig. S1 for a complete checklist of shredders collected in this study). The numbers of individuals of the three genera Nemoura, Protonemura and Amphinemura were used as dependent variables in our analyses, while we used the number of individuals of the other shredder taxa as a proxy for inter-specific competition.
Statistical analyses
All statistical analyses were performed with R software (R Core Team, 2019). In a preliminary step, we calculated the Froude number (Gordon et al., 1992) and the Substrate Index (SI, modified by Quinn & Hickey, 1994 after Jowett et al., 1991) to obtain synthetic measures of hydraulic stress and substrate composition. The Froude number is a measure of hydraulic turbulence, hence high values correspond to erosive microhabitats. It is calculated as: v/√(d × g), where v = flow velocity, measured as m/s, d = water depth, measured as m, and g is the gravity acceleration. The SI quantifies the coarseness of the substrate composition, with high values corresponding to coarse substrates and it is calculated as: 0.8 × %Rocks + 0.7 × %Boulders + 0.6 × %Cobbles + 0.5 × %Gravel + 0.4 × %Sand. We focused our attention on these parameters since they have already been successfully used to describe the physical niche of benthic invertebrates (e.g., Lamouroux et al., 2004; Mesa, 2010; Bo et al., 2017; Piano et al., 2019b).
Ecological niches of Nemouridae
In order to characterize the ecological niches of Nemouridae, we compared the overall (i.e., independent of streams and sections) ecological niches of the examined genera based on abundance data in both PS and IS sites to investigate whether their ecological requirements overlap, thus suggesting possible exploitative competition. To perform this, we calculated their ecological niche as their multidimensional Hutchinsonian hypervolume with a kernel density estimation (KDE) procedure via the hypervolume R package (Blonder, 2015) based on the Froude number, SI, CPOM mass and the number of other shredder taxa measured at each Surber sample. All variables were standardized before this analysis to achieve the same dimensionality for all axes and the hypervolume was calculated with the hypervolume_gaussian R command (Blonder, 2015), which constructs a hypervolume based on a Gaussian kernel density estimate. We standardized the choice of bandwidth for each variable through a Silverman estimator, and we set a threshold that included 100% of the total probability density. We then calculated the intersection between the hypervolumes and their overlap statistics for each pair of genera via the hypervolume_set and hypervolume_overlap_statistics R commands, respectively (Blonder, 2015). Overlap statistics include the Jaccard and Sorensen similarity indices, which range from 0 to 1 (0 = no overlap; 1 = complete overlap).
Effects of flow intermittency on the ecological niches of Nemouridae
To test the effect of flow intermittency on the ecological niches of Nemouridae, we first checked whether environmental and biotic parameters, namely Froude number, SI, CPOM mass and the abundance of individuals belonging to shredders (hereinafter Competition), and abundances of the three examined genera differed between PSs and ISs with the non-parametric Wilcoxon test repeated for each variable.
In a second step, we assessed whether flow intermittency affects the ecological niche of the three examined genera by means of the Outlying Mean Index (OMI) analysis performed on PS and IS sites separately. The OMI is a two-table ordination technique that can be implemented even with low number of individuals, such as those observed in our work. It positions the sampling units in a multidimensional space as a function of environmental parameters and the distribution of species in this space represents their realized niches (Dolédec et al., 2000). It is based on the concept of marginality, i.e., the distance between the mean habitat conditions observed in the sampling sites where the taxon is present and the mean habitat conditions across the study area. Taxa with high marginality occur in atypical habitats within the study area, while those with low marginality occur in typical habitats within the study area. Besides the marginality, the OMI analysis also calculates the tolerance of each taxon, which is calculated as the niche breadth, namely the amplitude in the distribution of each species along the sampled environmental gradients. Low tolerance values mean that a species is distributed across a limited range of conditions (specialist species), while high tolerance values imply that a species is distributed across habitats with widely varying environmental conditions (generalist species). The OMI analysis were performed via the function “niche” in the package ade4 (Chessel et al., 2004; Dray & Dufour, 2007; Dray et al., 2007) for the R software (R Core Team, 2019).
Results
All the three genera of Nemouridae typical of the Alpine area, namely Nemoura, Protonemura and Amphinemura, were present in the samples and were generally more abundant in PS than in IS sites. We collected 492 Nemouridae, 450 individuals in PS sites and 72 individuals in IS sites, recorded in 11 and 6 reaches, respectively. Among the three genera, Protonemura resulted the most abundant and widely distributed genus, with 264 individuals recorded in 13 out of 28 reaches, with higher occurrence and abundance in PS (Occurrence = 8 reaches; Abundance = 225 individuals) than IS sites (Occurrence = 5 reaches; Abundance = 39 individuals). Amphinemura was slightly less abundant than Protonemura, with a total of 236 individuals of Amphinemura, 205 in PS sites and 31 in IS sites, recorded in 10 reaches and 4 reaches, respectively, for a total of 14 reaches. Nemoura showed the lowest occurrence and abundance, with only 22 individuals collected, 20 in PS sites and 2 in IS sites, recorded in 8 and 2 reaches, respectively, for a total of 10 reaches. Of the 28 investigated reaches, 11 had no Nemouridae, 2 had only one genus, 10 hosted 2 genera and 5 reaches accounted for the three examined genera.
Ecological niches of Nemouridae
Among the three examined genera, Protonemura showed the highest dimension of the four-dimensional hypervolume (559.4), followed by Amphinemura (353.0) and Nemoura (248.8). The three hypervolumes partially overlap (Fig. 1) as demonstrated by the Jaccard and Sorensen similarity indices (Table 1). Although Nemoura has the smallest hypervolume, about 50% of its ecological niche is unique and does not overlap with that of Protonemura and Amphinemura (Unique 1 values at the first and second line, respectively in Table 1). The highest overlap value is observed between the ecological niches of Amphinemura and Protonemura (Table 1). The overlap between ecological niches (Fig. 1) indicates that the unique portion of Nemoura niche is mainly determined by the fact that it is not limited by the presence of other shredder taxa (Competition), but it seems the be the most limited by the amount of organic matter (CPOM). Amphinemura and Protonemura show an opposite pattern compared to Nemoura, as they occupy all ecological niches determined by CPOM while they are limited by the competition with other shredder taxa (Fig. 1). When considering the Froude number and the Substrate Index, the ecological niches of the three genera broadly overlap, suggesting that they have similar requirements in terms of hydromorphological conditions (Fig. 1).
Effects of flow intermittency on the ecological niches of Nemouridae
The Wilcoxon test highlighted significant differences among PS and IS sites for all the three genera, as PS sites had higher abundances and more favorable habitat conditions than in IS sites (Table 2), and among PS and IS sites in terms of Substrate Index, CPOM mass, and Competition, while no differences were observed for the Froude number (Table 2). In particular, our results highlight how PS sites have significant higher values of SI, CPOM and Competition than IS sites.
To evaluate how flow intermittency affects the ecological preferences of the three examined genera, we performed the OMI analysis, which showed how responses to hydrological intermittency seems to be genera-specific.
In PS sites, Amphinemura showed the highest marginality and tolerance values, thus being the genus that occupies the most marginal microhabitats, whereas Nemoura showed the lowest marginality, which means that it occupies the most typical microhabitats in permanent sites (Table 3). When considering tolerance, Amphinemoura showed the highest values, thus having the widest niche, while Protonemura showed the lowest tolerance values, thus being the genus with the narrowest niche in permanent sites (Table 3). In IS sites, Nemoura has the highest marginality, thus occupying the most marginal microhabitats in intermittent sites, whereas Protonemura has the lowest marginality value, thus occurring in the most typical microhabitats of intermittent sites (Table 3). When considering niche width, Amphinemura resulted the genus with the widest niche, as it had the highest tolerance value, whereas Protonemura has the narrowest niche as demonstrated by the fact that it has the lowest tolerance value (Table 3).
When considering the role of environmental variables, Nemoura was positively affected by Froude number (0.31), Competition (0.55), and weakly by CPOM mass (0.10), but negatively by SI (− 0.22) in PS sites (Table 4 and Fig. 2a), while it is positively affected by CPOM mass (0.23) and negatively by Competition (− 0.28), Froude (− 0.76) and SI (− 1.06) in IS sites (Table 4 and Fig. 2b). Protonemura was positively affected by Froude number (0.14) and negatively affected by SI (− 0.17), while it showed an extremely low influence of CPOM mass (− 0.06) and Competition (− 0.05) (Table 4 and Fig. 2a) in PS sites. In IS sites, it showed a positive relationship with CPOM (0.41) and SI (0.71), whereas it is negatively affected by Competition (− 0.11) and Froude (− 0.17) (Table 4 and Fig. 2b). Amphinemura is favored by Competition (0.61), followed by CPOM mass (0.45), and SI (0.38), but it is weakly negatively affected by Froude number (− 0.14) (Table 4 and Fig. 2a) in PS sites. In IS sites, it is positively correlated with CPOM mass (0.80), Froude (0.12) and SI (0.35), while it is not affected by Competition (− 0.01) (Table 4 and Fig. 2b).
Therefore, Nemoura shifts from reophilous microhabitats with finer substrates, high hydraulic stress and competition in permanent sites towards microhabitats with finer substrates, high CPOM availability and low competition and hydraulic stress in intermittent sites. Protonemura prefers microhabitats with finer substrates and high hydraulic stress in permanent sites, whereas it is mainly found in microhabitats with coarser substrates, high CPOM availability and low hydraulic stress and competition in intermittent sites. Amphinemura selects microhabitats with coarser substrates and high competition and CPOM availability in permanent sites, while it is not affected by competition in intermittent sites.
Discussion
We here evaluated the role of flow intermittency in shaping the niche of three coexisting phylogenetically related shredders in Alpine streams recently facing summer seasonal drying events. As summer drying may strongly affect the CPOM processing, by altering the decomposition by fungi and bacteria, and availability, as high fine sediment deposition buries CPOM, in the subsequent months, we here tested whether and how flow intermittency affects the three shredder genera belonging to the Nemouridae family, namely Nemoura, Protonemura and Amphinemura. By first highlighting how these three genera share similar ecological requirements, we then demonstrated a clear negative effect of flow intermittency on both the abundance and ecological preferences of Nemoura, Protonemura and Amphinemura.
Ecological niches of Nemouridae
As we expected differences in the ecological requirements of Nemouridae, we first examined whether the examined genera differed in the dimension of their realized ecological niches and whether they overlap in their ecological requirements. Our results displayed only a partial overlap of the niche hypervolumes of the three genera, partially confirming our first hypothesis. Although our model organisms were found to co-occur in similar hydromorphological conditions, they differ in their niche dimension especially in terms of CPOM availability and potential competitive pressure. Nemoura displayed the smallest niche, but it also showed the highest unique component, which can be due both to competitive exclusion and the capacity to exploit atypical habitats. The results of the OMI analysis support this second hypothesis as Nemoura occupied the most atypical habitats in intermittent sites. In addition, this genus is also possibly the most negatively affected by flow intermittency as its tolerance decreases from perennial to intermittent sites. We can hypothesize that this can be due to the fact that some species within this genus, such as Nemoura cinerea, display semivoltine populations (Fochetti et al., 2009). Voltinism has been recognized as one of the most sensitive traits (Bonada & Doledec, 2018) to flow intermittency as shifts from semivoltinism to multivoltinism have been observed from perennial to intermittent hydrological regimes in Mediterranean streams (López-Rodríguez et al. 2009a, b). In fact, plurivoltine species can overcome the negative effects of flow intermittency events on individuals inhabiting the stream during summer by producing multiple generations per year. Conversely, individuals semivoltine species, i.e., completing their life cycle in more than 1 year, have to face the drying up of the riverbed, thus being strongly affected by these events.
Although being highly sensitive to flow intermittency, Protonemura and Amphinemura resulted to be less influenced by flow intermittency than Nemoura, possibly because species belonging to these genera are mainly monovoltine (Fochetti et al., 2009), and their life cycle allows them to escape flow intermittency events by emerging before their occurrence. This hypothesis of a similar response of these two genera to flow intermittency is further supported by the fact that their ecological niches broadly overlap. Although having the widest niche, the results of the OMI analysis suggest that Protonemura is outcompeted by Amphinemura, which has the highest tolerance in both permanent and especially in intermittent sites. Although being negatively affected by flow intermittency, as demonstrated by the lower number of individuals in intermittent compared to permanent sites, Amphinemura was the most tolerant genus to flow intermittency among the three Nemouridae genus studied. This can be due to the lower body size dimensions of this genus compared to Nemoura and Protonemura (Fochetti et al., 2009) as small body size is also a common attribute of macroinvertebrate taxa living in intermittent streams because smaller individuals have display fast development and population growth, which allow to complete the life cycle before the water disappears (Bonada et al. 2007).
Effects of flow intermittency on the ecological niches of Nemouridae
When considering the effect of flow intermittency on the three examined genera, our results showed that abundances of the three genera were lower in intermittent than in permanent reaches, in agreement with our second hypothesis. This is in accordance with the results observed in a related study performed in the same study area, where we observed a significant negative effect of flow intermittency on the relative abundance of scrapers taxa (Piano et al., 2019a). This reduction is due to the lower availability of organic matter in intermittent than in permanent sites, which is in turn potentially determined by the lower retention capacity of the riverbed substrate in intermittent sites. In fact, although the flow had recovered since 6 months at the sampling moment, we still observed a significant lower value of the substrate index in intermittent sites, indicating that sites experiencing recurrent drying events are characterized with finer substrates than perennial sites. Heavy fine sediment accumulation (i.e., clogging) is strictly associated with flow reduction and droughts, because lower water velocity prevent the export of fine sediments (Dewson et al., 2007; Rolls et al., 2012), thus reducing the retention capacity of the substrate. In addition, the high fine sediment deposition can alter the quantity of energy inputs, as it affects the amount of autotrophic production (Henley et al., 2000; Bona et al., 2016) and allochthonous coarse organic matter availability in Alpine streams (Doretto et al., 2016, 2017). In particular, the burial of leaf litter by sediments reduces availability of this resource and alters the composition of the microbial community involved in its degradation (Receveur et al., 2020), with consequent negative effects on shredder invertebrates (Danger et al., 2012).
When focusing on the differences in ecological niches of the three genera between permanent and intermittent sites, the relationship with CPOM is weak in permanent sites, except for Amphinemura, but it becomes consistently and highly positive in intermittent sites, suggesting food limitation under flow intermittency conditions. Competition with other shredders positively influences the examined genera in permanent sites, except for Protonemura, suggesting that in permanent sites, where CPOM availability is high, different shredder taxa can coexist. Conversely, in intermittent sites, competition with other shredders has a consistent weak negative effect, possibly because the access to trophic resources is dominated by exploitative competition among shredder taxa due to food limitation, with detrimental effects on Nemouridae. Our suggestion is supported by Tierno de Figueroa & Lopez-Rodriguez (2019) reviewed how Nemouridae are highly dependent on CPOM, even if some species can act as collector-gatherers (Lopez-Rodriguez et al., 2010).
While the role of CPOM availability and competition in determining the ecological niches in permanent and intermittent sites is consistent among the three examined genera, the role of the hydromorphological parameters is more controversial. When considering the Froude number, Nemoura and Protonemura were favored in reophilous microhabitats in permanent but not in intermittent sites, whereas Amphinemura was weakly affected by this factor. Our sampling sites are located in mountain streams, where near-bed hydraulic stress is naturally high. In these conditions, CPOM usually accumulates when particles hit an obstruction, such as a rock, log or vegetation, where the hydraulic stress is lower (Hoover et al., 2006; Quinn et al., 2007). Therefore, at microhabitat level, hydraulic conditions have an indirect effect on the examined genera, with high Froude numbers likely negatively affecting the occurrence of shredder taxa as often associated with low CPOM retention. We can suggest that in permanent sites, where there is no food limitation, Nemoura and Protonemura better thrive in fast flowing conditions, which represent optimal habitats for Nemouridae (Usseglio-Polatera et al., 2000), whereas in intermittent sites they select suboptimal habitats, where the CPOM concentration is expected to be higher (Quinn et al., 2007).
Given that microhabitats with low flow velocity are deposition areas not only of CPOM but also of fine sediments (Quinn et al., 2007), the substrate preference can also indirectly affect the examined genera by conditioning the availability of CPOM. In fact, we observed again contrasting results among the three examined genera, and even between permanent and intermittent sites for Protonemura, which could be ascribed to the relationship between CPOM availability and substrate composition. In fact, Protonemura is mainly found on finer substrates in permanent sites whereas it shifts on coarser substrates in intermittent sites. This change can be due to the food limitation in intermittent sites, where Protonemura selects microhabitat with coarser substrates that have a higher retention capacity of CPOM. The relationship of Nemoura and Amphinemura with this parameter corroborates this hypothesis, as Amphinemura, which shows the stronger association with CPOM in both permanent and intermittent sites, has a consistent positive relationship with the Substrate Index, whereas Nemoura, which is the genus with the lowest association with CPOM in both permanent and intermittent sites, consistently prefers finer substrates.
Conclusions
Overall, our results emphasize how stream physical parameters, resource availability and their interaction play a key role in determining the distribution and the ecological niche of shredders in Alpine streams. Recurrent drying events negatively affect the examined genera, which are less represented in ISs than in PSs, and the narrower niches in ISs than in PSs, and their niche shift in ISs furtherly corroborate this hypothesis. According to our results, the negative effect of flow intermittency is mainly due to the reduced availability of CPOM compared to permanent sites confirming previous findings in other temperate geographical areas (Datry et al., 2011; Pinna et al., 2016). Consequently, water flow reduction and recurrent drying events are expected to reduce shredder biodiversity by altering the availability of their energetic inputs, with potential dramatic effects on stream ecosystem functionality (e.g., Ledger et al., 2008; Datry et al., 2011; Piano et al., 2019b). In fact, the expected decrease in allochthonous trophic resources will likely cause a bottom-up effect in the food web, directly influencing the survival, growth and reproduction of invertebrate shredders. Further investigations in mountain areas are thus required in the next future to better unravel how the interaction between flow intermittency and CPOM availability may affect shredders in lotic ecosystems.
References
Abril, M., I. Muñoz & M. Menéndez, 2016. Heterogeneity in leaf litter decomposition in a temporary Mediterranean stream during flow fragmentation. Science of The Total Environment 553: 330–339.
Agenzia Regionale per la Protezione dell’Ambiente, 2013. Idrologia in Piemonte nel 2012. Regione Piemonte.
Belmar, O., D. Bruno, S. Guareschi, A. Mellado-Díaz, A. Millán & J. Velasco, 2019. Functional responses of aquatic macroinvertebrates to flow regulation are shaped by natural flow intermittence in Mediterranean streams. Freshwater Biology 64: 1064–1077.
Benstead, J. P. & A. D. Huryn, 2011. Extreme seasonality of litter breakdown in an arctic spring-fed stream is driven by shredder phenology, not temperature. Freshwater Biology 56: 2034–2044.
Blonder, B., 2015. hypervolume: High-Dimensional Kernel Density Estimation and Geometry Operations. - R package version 1.2.2., http://CRAN.R-project.org/package=hypervolume
Bo, T., M. J. López-Rodríguez, A. Mogni, J. M. Tierno de Figueroa & S. Fenoglio, 2013. Life history of Capnia bifrons (Newman, 1838) (Plecoptera: Capniidae) in a small Apennine creek, NW Italy. Entomologica Fennica 24: 29–34.
Bo, T., E. Piano, A. Doretto, F. Bona & S. Fenoglio, 2017. Microhabitat preference of sympatric Hydraena Kugelann, 1794 species (Coleoptera: Hydraenidae) in a low-order forest stream. Aquatic Insects 37: 287–292.
Bona, F., A. Doretto, E. Falasco, V. La Morgia, E. Piano, R. Ajassa & S. Fenoglio, 2016. Increased sediment loads in alpine streams: an integrated field study. River Research and Applications 32: 1316–1326.
Bonada, N. & S. Dolédec, 2018. Does the Tachet trait database report voltinism variability of aquatic insects between Mediterranean and Scandinavian regions? Aquatic Sciences 80: 7.
Bonada, N., M. Rieradevall & N. Prat, 2007. Macroinvertebrate community structure and biological traits related to flow permanence in a Mediterranean river network. Hydrobiologia 589: 91–106.
Boyero, L., R. G. Pearson, D. Dudgeon, V. Ferreira, M. A. Graça, M. O. Gessner, J. A. Boulton, E. Chauvet, C. M. Yule, R. J. Albariño, A. Ramírez, J. E. Helson, M. Callisto, M. Arunachalam, J. Chará, R. Figueroa, J. M. Mathooko, J. F. Gonçalves, M. S. Moretti, A. M. Chará-Serna, J. N. Davies, A. Encalada, S. Lamothe, L. M. Buria, J. Castela, A. Cornejo, A. O. Y. Li, C. M’Erimba, V. Díaz Villanueva, M. C. Zúñiga, C. M. Swan & L. A. Barmuta, 2012. Global patterns of stream detritivore distribution: implications for biodiversity loss in changing climates. Global Ecology and Biogeography 21: 134–141.
Brighenti, S., M. Tolotti, M. C. Bruno, G. Wharton, M. T. Pusch & W. Bertoldi, 2019. Ecosystem shifts in Alpine streams under glacier retreat and rock glacier thaw: a review. The Science of the Total Environment 675: 542–559.
Bruno, M. C., A. Siviglia, M. Carolli & B. Maiolini, 2013. Multiple drift responses of benthic invertebrates to interacting hydropeaking and thermopeaking waves. Ecohydrology 6: 511–522.
Bruno, D., O. Belmar, A. Maire, A. Morel, B. Dumont & T. Datry, 2019. Structural and functional responses of invertebrate communities to climate change and flow regulation in alpine catchments. Global Change Biology 25: 1612–1628.
Calapez, A. R., C. L. Elias, S. F. Almeida & M. J. Feio, 2014. Extreme drought effects and recovery patterns in the benthic communities of temperate streams. Limnetica 33: 281–296.
Campaioli, S., P. F. Ghetti, A. Minelli & S. Ruffo, 1999. Manuale per il riconoscimento dei macroinvertebrati delle acque dolci italiane, Vol. II. Provincia Autonoma di Trento, Trento, IT.
Campaioli, S., P. F. Ghetti, A. Minelli & S. Ruffo, 1994. Manuale per il riconoscimento dei macroinvertebrati delle acque dolci italiane, Vol. I. Provincia Autonoma di Trento, Trento, IT.
Chessel, D., A. B. Dufour & J. Thioulouse, 2004. The ade4 package-I: one-table methods. R News. 4: 5–10.
Corti, R., T. Datry, L. Drummond & S. T. Larned, 2011. Natural variation in immersion and emersion affects breakdown and invertebrate colonization of leaf litter in a temporary river. Aquatic Sciences 73: 537–550.
Cummins, K. W., M. A. Wilzbach, D. M. Gates, J. B. Perry & W. B. Taliaferro, 1989. Shredders and riparian vegetation. BioScience 39: 24–30.
Danger, M., J. Cornut, A. Elger & E. Chauvet, 2012. Effects of burial on leaf litter quality, microbial conditioning and palatability to three shredder taxa. Freshwater Biology 57: 1017–1030.
Datry, T., R. Corti, C. Claret & M. Philippe, 2011. Flow intermittence controls leaf litter breakdown in a French temporary alluvial river: the “drying memory”. Aquatic Sciences 73: 471–483.
Dewson, Z. S., A. B. James & R. G. Death, 2007. A review of the consequences of decreased flow for instream habitat and macroinvertebrates. Journal of North American Benthological Society 26: 401–415.
Dolédec, S., D. Chessel & C. Gimaret-Carpentier, 2000. Niche separation in community analysis: a new method. Ecology 81: 2914–2927.
Doretto, A., F. Bona, E. Falasco, E. Piano, P. Tizzani & S. Fenoglio, 2016. Fine sedimentation affects CPOM availability and shredder abundance in Alpine streams. Journal of Freshwater Ecology 31: 299–302.
Doretto, A., F. Bona, E. Piano, I. Zanin, A. C. Eandi & S. Fenoglio, 2017. Trophic availability buffers the detrimental effects of clogging in an alpine stream. Science of the Total Environment 592: 503–511.
Doretto, A., E. Piano, E. Falasco, S. Fenoglio, M. C. Bruno & F. Bona, 2018. Investigating the role of refuges and drift on the resilience of macroinvertebrate communities to drying conditions: an experiment in artificial streams. River Research and Applications 34: 777–785.
Doretto, A., F. Bona, E. Falasco, D. Morandini, E. Piano & S. Fenoglio, 2020a. Stay with the flow: how macroinvertebrate communities recover during the rewetting phase in Alpine streams affected by an exceptional drought. River Research and Applications 36: 91–101. https://doi.org/10.1002/rra.3563.
Doretto, A., T. Bo, F. Bona & S. Fenoglio, 2020b. Efficiency of Surber net under different substrate and flow conditions: insights for macroinvertebrates sampling and river biomonitoring. Knowledge and Management of Aquatic Ecosystems 421: 10.
Dray, S. & A. B. Dufour, 2007. The ade4 package: implementing the duality diagram for ecologists. Journal of Statistical Software 22: 1–20.
Dray, S., A. B. Dufour & D. Chessel, 2007. The ade4 package-II: two-table and K-table methods. R News 7: 47–52.
Elias, C. L., A. R. Calapez, S. F. Almeida & M. J. Feio, 2015. From perennial to temporary streams: an extreme drought as a driving force of freshwater communities’ traits. Marine and Freshwater Resources 66: 469–480.
Falasco, E., E. Piano, A. Doretto, S. Fenoglio & F. Bona, 2018. Lentification in Alpine rivers: patterns of diatom assemblages and functional traits. Aquatic Sciences 80: 36.
Fenoglio, S., T. Bo, P. Agosta & G. Malacarne, 2005. Temporal and spatial patterns of coarse particulate organic matter and macroinvertebrate distribution in a low-order Apennine stream. Journal of Freshwater Ecology 20: 539–547.
Fenoglio, S., T. Bo, M. Cucco & G. Malacarne, 2007. Response of benthic invertebrate assemblages to varying drought conditions in the Po river (NW Italy). Italian Journal of Zoology 74: 191–201.
Fenoglio, S., T. Bo, M. Cucco, L. Mercalli & G. Malacarne, 2010. Effects of global climate change on freshwater biota: a review with special emphasis on the Italian situation. Italian Journal of Zoology 77: 374–383.
Fenoglio, S., T. Bo, M. Cammarata, M. J. López-Rodríguez & J. M. Tierno de Figueroa, 2014. Seasonal variation of allochthonous and autochthonous energy inputs in an alpine stream. Journal of Limnology 74: 272–277.
Ferreira, V., A. V. Lírio, J. Rosa & C. Canhoto, 2013. Annual organic matter dynamics in a small temperate mountain stream. Annales de Limnologie-International Journal of Limnology 49: 13–19.
Fochetti, R., C. A. Ravizza & J. T. De Figueroa, 2009. Plecoptera, Vol. 43. Fauna d’Italia, Calderini.
González, J. M. & M. A. S. Graça, 2003. Conversion of leaf litter to secondary production by a shredding caddis-fly. Freshwater Biology 48: 1578–1592.
Gordon, N. D., T. A. McMahon & B. L. Finlayson, 1992. Stream Hydrology: An Introduction for Ecologists. Wiley, Chichester.
Henley, W. F., M. A. Patterson, R. J. Neves & A. D. Lemly, 2000. Effects of sedimentation and turbidity on lotic food webs: a concise review for natural resource managers. Review in Fisheries Science 8: 125–139.
Hoover, T. M., J. S. Richardson & N. Yonemitsu, 2006. Flow–substrate interactions create and mediate leaf litter resource patches in streams. Freshwater Biology 51: 435–447.
Jowett, I. G., J. Richardson, B. J. Biggs, C. W. Hickey & J. M. Quinn, 1991. Macrohabitat preferences of benthic invertebrates and the development of generalized Deleatidium spp. habitat suitability curves, applied to four New Zealand rivers. New Zealand Journal of Marine and Freshwater Research 25: 187–200.
Lamouroux, N., S. Dolédec & S. Gayraud, 2004. Biological traits of stream macroinvertebrate communities: effects of microhabitat, reach, and basin filters. Journal of the North American Benthological Society 23: 449–466.
Ledger, M. E., R. M. Harris, P. D. Armitage & A. M. Milner, 2008. Disturbance frequency influences patch dynamics in stream benthic algal communities. Oecologia 155: 809–819.
López-Rodríguez, M. J., J. M. Tierno de Figueroa, S. Fenoglio, T. Bo & J. Alba-Tercedor, 2009a. Life strategies of 3 Perlodidae species (Plecoptera) in a Mediterranean seasonal stream in southern Europe. Journal of the North American Benthological Society 28: 611–625.
López-Rodríguez, M. J., J. M. Tierno de Figueroa & J. Alba-Tercedor, 2009b. The life history of Serratella ignita (Poda, 1761)(Insecta: Ephemeroptera) in a temporary and permanent Mediterranean stream. Aquatic Sciences 71: 179–188.
López-Rodríguez, M. J., T. Bo, J. M. Tierno De Figueroa & S. Fenoglio, 2010. Nymphal trophic behaviour of two Nemouridae species (Insecta, Plecoptera) in the Curone creek (northern Apennines, Italy). Entomological Science 13: 288–292.
McGregor, G., G. E. Petts, A. M. Gurnell & A. M. Milner, 1995. Sensitivity of alpine stream ecosystems to climate change and human impacts. Aquatic Conservation: Marine and Freshwater Ecosystems 5: 233–247.
Merritt, R. W., K. W. Cummins & M. B. Berg, 2017. Trophic relationships of macroinvertebrates. In Hauer, F. R. & G. A. Lamberti (eds), Methods in Stream Ecology, 3rd ed. Academic Press, London: 413–433.
Mesa, L. M., 2010. Hydraulic parameters and longitudinal distribution of macroinvertebrates in a subtropical Andean basin. Interciencia 35: 760–764.
Milner, A. M., K. Khamis, T. J. Battin, J. E. Brittain, N. E. Barrand, L. Füreder, S. Cauvy-Fraunié, G. M. Gíslason, D. Jacobsen, D. M. Hannah, A. J. Hodson, E. Hood, V. Lencioni, J. S. Ólafsson, C. T. Robinson, M. Tranter & L. E. Brown, 2017. Glacier shrinkage driving global changes in downstream systems. Proceedings of the National Academy of Sciences 114: 9770–9778.
Petersen, R. C. & K. W. Cummins, 1974. Leaf processing in a woodland stream. Freshwater Biology 4: 343–368.
Piano, E., A. Doretto, E. Falasco, S. Fenoglio, L. Gruppuso, D. Nizzoli, P. Viaroli & F. Bona, 2019a. If Alpine streams run dry: the drought memory of benthic communities. Aquatic Sciences 81: 32.
Piano, E., A. Doretto, E. Falasco, L. Gruppuso, S. Fenoglio & F. Bona, 2019b. The role of recurrent dewatering events in shaping ecological niches of scrapers in intermittent Alpine streams. Hydrobiologia 841: 177–189.
Pinna, M., G. Marini, G. Cristiano, L. Mazzotta, P. Vignini, B. Cicolani & A. Di Sabatino, 2016. Influence of aperiodic summer droughts on leaf litter breakdown and macroinvertebrate assemblages: testing the drying memory in a Central Apennines River (Aterno River, Italy). Hydrobiologia 782: 111–126.
Quinn, J. M. & C. W. Hickey, 1994. Hydraulic parameters and benthic invertebrate distributions in two gravel-bed New Zealand rivers. Freshwater Biology 32: 489–500.
Quinn, J. M., N. R. Phillips & S. M. Parkyn, 2007. Factors influencing retention of coarse particulate organic matter in streams. Earth Surface Processes and Landforms 32: 1186–1203.
R Core Team ver. 5.3.5, 2019. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.
Receveur, J. P., S. Fenoglio & M. E. Benbow, 2020. Insect-associated bacterial communities in an alpine stream. Hydrobiologia 847: 331–344.
Rolls, R. J., C. Leigh & F. Sheldon, 2012. Mechanistic effects of low-flow hydrology on riverine ecosystems: ecological principles and consequences of alteration. Freshwater Science 31: 1163–1186.
Tachet, H., P. Richoux, M. Bournaud & P. Usseglio-Polatera, 2010. Invertébrés d’eau douce: Systématique, biologie, écologie, Vol. 15. CNRS Editions, Paris.
Taylor, B. R. & I. V. Andrushchenko, 2014. Interaction of water temperature and shredders on leaf litter breakdown: a comparison of streams in Canada and Norway. Hydrobiologia 721: 77–88.
Tierno de Figueroa, J. M., T. Bo, M. J. López-Rodríguez & S. Fenoglio, 2009. Life cycle of three stonefly species (Plecoptera) from an Apenninic stream (Italy) with the description of the nymph of Nemoura hesperiae. Annales de la Societé Entomologique de France 45: 339–343.
Tierno de Figueroa, J. M. & M. J. López-Rodríguez, 2019. Trophic ecology of Plecoptera (Insecta): a review. The European Zoological Journal 86: 79–102.
Usseglio-Polatera, P., M. Bournaud, P. Richoux & H. Tachet, 2000. Biological and ecological traits of benthic freshwater macroinvertebrates: relationships and definition of groups with similar traits. Freshwater Biology 43: 175–205.
Vannote, R. L., G. W. Minshall, K. W. Cummins, J. R. Sedell & C. E. Cushing, 1980. The river continuum concept. Canadian Journal of Fisheries and Aquatic Sciences 37: 130–137.
Wasson, J. G., A. Chandesris, A. G. Bautista, H. Pella & B. Villeneuve, 2007. REBECCA, Relationships between ecological and chemical status of surface waters. European Hydro-Ecoregions. EU 6th Framework Programme. Cemagref Institute, Lyon: 43.
Webster, J. R. & J. L. Meyer, 1997. Organic matter budgets for streams: a synthesis. Journal of the North American Benthological Society 16: 141–161.
Acknowledgements
The authors wish to thank the two anonymous reviewers for their suggestions and positive critics that highly contributed to the improvement of this work. We thank M. Apostolo, R. Bolpagni, M. C. Bruno, G. Burgazzi, C. Garetto, A. Laini, D. Melchio, D. Morandini, D. Nizzoli and B. Palmia for their assistance and contribution during the field and laboratory activities. Marco Baltieri (ATAAI - Associazione Tutela Ambienti Acquatici e Ittiofauna) is greatly acknowledged for his help in the identification of temporary sampling reaches. This work was realized within the framework of the PRIN NOACQUA “Risposte di comuNità e processi ecOsistemici in corsi d’ACQUA soggetti a intermittenza idrologica” - code 2O1572HW8F, funded by the Italian Ministry of Education, University and Research, and is part of the research fellowship “Aquatic invertebrate communities as sentinels of climate change in Italian Alpine streams” funded by Fondazione CRT and as part of the activities of ALPSTREAM, a research center financed by FESR, Interreg Alcotra 2014-2020, Project n 4083 - EcO of the Piter Terres Monviso.
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Piano, E., Doretto, A., Falasco, E. et al. Flow intermittency negatively affects three phylogenetically related shredder stoneflies by reducing CPOM availability in recently intermittent Alpine streams in SW-Italian Alps. Hydrobiologia 847, 4049–4061 (2020). https://doi.org/10.1007/s10750-020-04399-4
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DOI: https://doi.org/10.1007/s10750-020-04399-4