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Insect-associated bacterial communities in an alpine stream

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Abstract

The roles of macroinvertebrate and microbial communities in stream ecosystems are recognized to be important to energy flow and nutrient cycling. While the linkages of these major groups of aquatic organisms have not been thoroughly investigated, determining how they interact is particularly important for understanding the mechanisms and potential evolutionary relationships that contribute to ecosystem processes, such as organic matter decomposition. We evaluated the microbiomes of several aquatic insect species differing in trophic ecology and belonging to different functional feeding groups at two sites along an Italian Alpine river with different elevation and environmental characteristics, one located above the tree line and the other in a forested environment. We found that the internal microbial communities of the different species significantly varied in taxonomic and functional composition and could be used to classify samples to both species and environment. We demonstrated that functional differences existed between the microbiota of different insect species with variable feeding behaviors, and that species differences were more important, in this context, than environmental or habitat conditions. These results provide new information on how the microbiomes of aquatic insects may potentially be influenced by their hosts and habitat conditions in Alpine streams.

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Acknowledgements

The authors would like to thank Courtney Weatherbee for assistance in processing insect specimens as well as the anonymous reviewers whose thoughtful comments greatly improved the manuscript.

Funding

Partial funding was provided by the College of Agriculture and Natural Resources, the Department of Entomology and AgBioResearch at Michigan State University.

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Authors and Affiliations

  1. Department of Entomology, Michigan State University, East Lansing, MI, USA

    Joseph P. Receveur & M. Eric Benbow

  2. Ecology, Evolutionary Biology and Behavior Program, Michigan State University, East Lansing, MI, USA

    Joseph P. Receveur & M. Eric Benbow

  3. Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Alessandria, Italy

    Stefano Fenoglio

  4. Centro per lo Studio dei Fiumi Alpini (ALPSTREAM - Alpine Stream Research Center), Ostana, Italy

    Stefano Fenoglio

  5. Department of Osteopathic Medical Specialties, Michigan State University, East Lansing, MI, USA

    M. Eric Benbow

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  1. Joseph P. Receveur
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Receveur, J.P., Fenoglio, S. & Benbow, M.E. Insect-associated bacterial communities in an alpine stream. Hydrobiologia 847, 331–344 (2020). https://doi.org/10.1007/s10750-019-04097-w

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