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Genetic variation of a freshwater snail Hydrobioides nassa (Gastropoda: Bithyniidae) in Thailand examined by mitochondrial DNA sequences

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Abstract

In Thailand, there are at least ten species of freshwater snails of the family Bithyniidae, most of which can act as intermediate hosts of veterinary and medically important parasites. The genetic variation, geographical distribution, and taxonomic status of some species are obscure, including for Hydrobioides nassa. Thus, this study aims to explore the genetic variation and distribution of H. nassa in north, west, and central Thailand. We collected 264 specimens of H. nassa from 46 localities in 16 provinces using mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA sequences to determine variation. Genetic diversity of H. nassa is relatively high, with 65 and 11 haplotypes of COI and 16S rDNA observed, respectively. The phylogenetic tree and haplotype network analyses classified H. nassa into three haplogroups (haplogroup I–III). Haplogroup I and II belonged to clade A, whereas haplogroup III belonged to clade B. Interestingly, haplogroup III or clade B contained the specimens from Yom river basin in Phrae province, which was the most genetically distinct. Thus, this study suggests that H. nassa in Thailand is a complex of phenotypically similar but genetically distinct species.

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Acknowledgements

This study was supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. program (Grant No. PHD/0095/2558) to Naruemon Bunchom. Thammasat University financial supports this project to Chairat Tantrawatpan. Takeshi Agatsuma was supported by Grants-in-Aid for Scientific Research (B) (26305011). The authors would like to thank the Mahasarakham University for financially supporting Weerachai Saijuntha, enabling him to present this work in the first FMCS International Freshwater Mollusk Meeting 2018, Verbania, Italy. The authors would also like to thank Dr. Wittaya Tawong for BI analysis.

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Authors and Affiliations

  1. Biodiversity and Conservation Research Unit, Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, 44150, Thailand

    Naruemon Bunchom, Weerachai Saijuntha, Warayutt Pilap & Warong Suksavate

  2. Department of Veterinary Technology, Faculty of Agricultural Technology, Rajabhat Maha Sarakham University, Maha Sarakham, 44000, Thailand

    Kotchaphon Vaisusuk

  3. Department of Environmental Medicine, Kochi Medical School, Kochi University, Nankoku, 783-8505, Japan

    Narufumi Suganuma & Takeshi Agatsuma

  4. Department of Paleontology and Evolution, State Museum of Natural History Karlsruhe, Erbprinzenstrasse 13, 76133, Karlsruhe, Germany

    Trevor N. Petney

  5. Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, 12120, Thailand

    Chairat Tantrawatpan

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  1. Naruemon Bunchom
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Correspondence to Weerachai Saijuntha.

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Bunchom, N., Saijuntha, W., Pilap, W. et al. Genetic variation of a freshwater snail Hydrobioides nassa (Gastropoda: Bithyniidae) in Thailand examined by mitochondrial DNA sequences. Hydrobiologia 848, 2965–2976 (2021). https://doi.org/10.1007/s10750-019-04013-2

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