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What scares a mussel? Changes in valve movement pattern as an immediate response of a byssate bivalve to biotic factors

Abstract

Responsiveness to biotic factors is crucial for the survival of sessile aquatic animals. They cannot escape from danger, but developed a number of defences against predation, usually delayed in time. We checked the initial defence of the freshwater byssate zebra mussel, Dreissena polymorpha, associated with valve gaping. We tested the effect of chemical signals: fish predator scent (the roach Rutilus rutilus), conspecific alarm cue and a mixture of both, as well as a mechanical stimulus: the presence of an amphipod (Dikerogammarus villosus) mechanically irritating mussels. The alarm cues and amphipod presence made mussels spend more time with closed/narrowly open valves, which can be related to decreasing detection probability by reduced infochemical excretion and/or protecting soft tissues in the presence of an imminent threat. In contrast, reactions to the predator scent alone were much weaker. Moreover, the fish scent mixed with alarm substance induced weaker responses than the alarm substance alone. Thus, the fish infochemical might mask the presence of the alarm cue components, potentially benefiting the predator. A variety of defences exhibited by mussels demonstrates the importance of the predation cue type (direct/indirect, chemical/mechanical, originating from conspecifics/predators/mixed) for the behaviour of sessile animals.

Introduction

Aquatic sessile species are ecologically and economically important as ecosystem engineers, fouling community members and nuisance for submerged equipment (Khalaman, 2001; Sousa et al., 2009). Their responses to environmental factors differ from those exhibited by mobile animals. With no or limited relocation capabilities they can react by withdrawing into their shells or shelters and modifying their activity (Reimer & Tedengren, 1997; Kobak et al., 2010; Naddafi & Rudstam, 2013). On the other hand, prolonged activity is crucial for their survival as most of them are filter-feeders, relying on constant pumping of water into their filtering structures (Gosling, 2003).

One of the factors most affecting animal behaviour is the presence of predators (Lima & Dill, 1990; Ferrari et al., 2009). Common defensive reactions of animals are active avoidance (Lima & Dill, 1990; Wisenden & Millard, 2001; Jermacz et al., 2015) and/or taking an appropriate shelter (Schmidt-Drewello et al., 2016). However, sessile organisms developed more sophisticated and less conspicuous responses. Depending on evolutionary constraints and predator hunting modes, they can more strongly adhere to the substratum (Shin et al., 2008; Cheung et al., 2009), reduce feeding rates (Naddafi et al., 2007), develop harder shells (Czarnołęski et al., 2006), produce toxins (Hill et al., 2005) and/or select sheltered sites during their mobile phase of life (Bryan et al., 1998; Dahms et al., 2004). These responses may vary depending on the quality of the predation cue, ranging from predator kairomones (depending on their diet and hunger level) to alarm substances produced by wounded conspecifics (indicating a more direct danger: a currently foraging predator) (Smee & Weissburg, 2006; Large et al., 2012; Jermacz et al., 2017). Furthermore, prey reactions vary with time, different measures being induced immediately after the detection of predation cues and during the chronic exposure to predator pressure (Reimer & Tedengren, 1997; Turner et al., 2006).

Moreover, sessile species are exposed to the impact of mobile non-predatory organisms occupying their colonies in great numbers (Botts et al., 1996). Being attached to the substratum, they have limited capability of avoiding scraping and tickling by specimens moving over their bodies, which can negatively affect their activity and condition, sometimes resembling the effect of predation cues (Kobak et al., 2012). Both predators and cohabiting organisms form a natural biotic environment of sessile organisms. In the field, various stimuli may create conflicting situations (when protection against one danger increases exposure to another) or act synergistically, optimizing responses to different risk sources (Wudkevich et al., 1997; Beggel et al., 2016). Therefore, the knowledge how they respond to these factors is crucial for understanding their functioning, impact on the environment and relationships with other species.

One of the most common freshwater sessile species is the zebra mussel, Dreissena polymorpha (Pallas, 1771). Although a hard shell defends it from most of its potential predators, it constitutes a considerable diet item for several fishes (Prejs et al., 1990; Naddafi & Rudstam, 2013). In the presence of predators, mussels increase their attachment (Kobak et al., 2010; Naddafi & Rudstam, 2013), aggregation (Naddafi & Rudstam, 2013) and shell strength (Czarnołęski et al., 2006; Naddafi & Rudstam, 2014). These changes increase predator handling costs (Prejs et al., 1990; Czarnołęski et al., 2006), but develop over a relatively long time (> 4 days) (Kobak & Kakareko, 2009; Kobak et al., 2012; Naddafi & Rudstam, 2013, 2014). Quite similar responses of zebra mussels (increase in attachment strength, reduction in upward movement) to the presence of gammarid amphipods occupying mussel colonies were also observed (Kobak et al., 2012). These animals are not considered a direct threat to mussels, but they may mechanically irritate mussel soft tissues and affect their responses to other environmental factors. The unresolved question is whether and how D. polymorpha reacts to these nuisance cues immediately after the signal reception. To answer this question, we checked mussel valve movements. They are associated with all life activities of mussels, such as nutrition, respiration, reproduction, excretion and signal reception (Gosling, 2003; García-March et al., 2008). Their feeding efficiency is directly related to the degree of valve gaping, regulating the pumping rate (Jørgensen et al., 1988; Newell et al., 1998; Riisgård et al., 2003; Saurel et al., 2007). In optimal environmental conditions, a mussel spends most of the time with open valves (Jørgensen et al., 1988; Kramer & Foekema, 2001). Thus, prolonged disturbance in valve opening may negatively affect mussel condition. In urgent stress situations, one of the fastest mussel reactions is valve closing (Borcherding, 2006), which reduces its contacts with the environment: both in terms of intaking potentially harmful substances (Rajagopal et al., 1997) as well as releasing chemical and mechanical cues that could be used by enemies to locate the sender (Antoł et al., 2018). Thus, reduction in valve gaping can be a potential defensive response regardless of the predator hunting mode.

In the present study, we checked short-time valve movement responses of D. polymorpha to predation cues and physical presence of non-predatory organisms occupying mussel beds. We tested mussel reactions to the kairomone of the roach Rutilus rutilus (Linnaeus, 1758). This fish is an efficient molluscivore (Prejs et al., 1990) and defensive responses of D. polymorpha to its kairomones have been found in previous studies (e.g. Naddafi et al., 2007; Kobak et al., 2010). The fish were fed with mussels or neutral food (chironomid larvae) to check for the potential impact of dietary cues in predator exudates (Chivers et al., 1996; Wisenden, 2015). Predation is connected with alarm substances released by crushed prey; thus, we also used this stimulus in our study. We hypothesized that all these chemical stimuli would decrease time spent with widely open valves and frequency of valve movements, which would be beneficial in terms of “chemical hiding” from a predator (Antoł et al., 2018). We also intended to check whether a mixture of predation cues (conspecific alarm substance, predator dietary cues and/or kairomone) would increase synergistically the magnitude of mussel reactions. Additionally, we checked the effects of the presence of a Ponto-Caspian gammarid Dikerogammarus villosus (Sowinsky, 1894), occupying D. polymorpha colonies (Devin et al., 2003). We supposed that gammarids might irritate soft parts of siphons and mantle surface and finally induce valve closing similarly to the aforementioned predation cues. If so, we intended to compare the relative strength of responses to predation and gammarid cues.

Materials and methods

Animal collection and housing

All experiments were conducted under laboratory conditions, using D. polymorpha, roach R. rutilus and gammarids collected from the Włocławek Reservoir (a dam lake located on the lower Vistula River, Central Poland) from a depth of ca. 2 m (flow velocity < 10 cm/s, Gierszewski, 2006) in October 2015 (temperature 10 °C). D. polymorpha and D. villosus occupying mussel colonies were sampled from the reservoir bottom by a diver together with their substrata (unionid bivalves). D. polymorpha were removed from the unionids in situ and the later were immediately returned to the environment. Roach were captured by electrofishing, using a certified device IUP-12 (Radet, Poznań, Poland). Other fish were released after capture and recovered within a few minutes. Fish collection and use in the experiments were conducted under permit from the Local Ethic Committee (University of Science and Technology, Bydgoszcz, Poland) no. 21/2015. Animals were transported to the laboratory (2 h transport time) in 10-l aerated plastic containers. All animals were kept for 2 weeks in single-species stock aquaria (all mussels: 350 L, 3 fish per 200 L, all gammarids: 150 L) supplied with aquarium filters and aerators. The water in aquaria had a constant temperature of 18 °C (sustained by air-conditioning). Mussels were fed every second day with 2 g of dried Chlorella sp. per 1000 individuals (Kilgour & Baker, 1994). Fish were fed according to the experimental treatment (see below) and gammarids were fed every day with 4 g of frozen chironomid larvae. Ca. 30% of the water volume was changed on weekly basis and on the occasion of water collection for the purpose of the experiment (see below).

Experimental design

All tested mussels were collected from the top layer of druses formed on unionid shells to minimize the risk of their weak condition. Mussels were detached from their natural substrate by severing their byssal threads with a scalpel. 24 h before the experiments, mussels (length: 18–22 mm) were marked by a thin layer of a low-weight red plasticine (determined as the fastest and hence least invasive labelling method in preliminary trials), attached to the posterior part of one of the valves (Fig. 1). This mark allowed the behaviour analysis software to follow the video-recorded valve movements. Marked mussels were fastened to glass microscope slides with unreactive fast-binding methyl acrylic glue by the ventral surface of the non-marked valve to prevent them from relocating during the experiment (Fig. 1). In the wild, mussels are often immobilized by overgrowing conspecifics and spend most of their life attached to the substratum; thus, this situation is not unusual for them. All processed individuals were left to recover in a 150-l stock tank for 24 h in the same conditions as those in experimental tanks. Only mussels which produced byssus threads after this time were used in the experiments.

Fig. 1
figure 1

Experimental setup

The experiments were conducted in glass beakers (diameter: 140 mm, height: 72 mm) filled with 700 ml of water. A microscope slide with a single mussel was placed in the beaker and positioned so that the mussel was located in a horizontal plane. The experimental beakers were cleaned, water changed and new individuals used for each replicate. Experimental treatments were replicated 19–25 times. This variability depended on the availability of suitable mussels (byssally attached after marking and widely open at least once during the entire experimental period) and number of successful trials (without technical errors, such as accidental detachment of the plasticine marker during the trial). Individuals that did not extend their siphons were also removed from the analysis as it could result from their poor physical condition or injury during the pre-experimental handling. After the visual inspection of the recordings ca. 5% of all trials were rejected due to the aforementioned reasons including only few cases of individuals that did not open their valves.

Mussels were placed in the beakers filled with water containing a particular stimulus (see below) and exposed for the next 2 h 15 min. The first 15-min. period was considered as an acclimation time and excluded from the analysis. Disturbances resulting from handling during the stimulus application at the beginning of the experiment could interrupt mussel behaviour and in consequence responses to examined factors. During the next 2 h mussel valve movements were recorded from the top by video cameras (SNB-6004, Samsung, South Korea).

The experiment consisted of seven treatments: (1) the conspecific alarm substance, (2) the scent of roach (kairomone) fed with chironomid larvae, (3) the scent of roach fed with D. polymorpha (kairomone and conspecific dietary cues), (4) the alarm substance and scent of roach fed with chironomids, (5) the alarm substance and scent of roach fed with mussels, (6) the presence of D. villosus and (7) control treatment without any cues. The alarm substance was a colourless filtered mixture of water and manually crushed mussels in volumetric proportion of 4:7. One ml of the alarm substance was added to the experimental beaker filled with control water to obtain the active concentration according to Toomey et al. (2002). The scent of the roach was applied by adding 700 ml of water (i.e. the entire beaker volume) from a 200 L stock tank, in which three fish, 220 mm in length, had been incubated for 2 weeks, to the experimental beakers. The fish were fed once a day with 3 g of chironomid larvae or 3 D. polymorpha per individual. They were starved for 24 h before collecting water for the experiment to avoid the presence of signals from living mussels (not consumed yet) in the test. The control water was pre-conditioned for 2 weeks in the same tank as that used to incubate the fish, but devoid of mussels and predators. In the gammarid treatment, three D. villosus individuals (15–18 mm in length) were placed in the experimental arena and allowed to move freely among the mussels.

Water temperature (sustained by air-conditioning) during the experiments was 18 °C. Illumination (necessary due to video recording) provided by incandescent light was 65 lx. Oxygen concentrations (measured with a multiparameter benchtop meter, inoLab Multi 9620 IDS, WTW, Poland connected with an optical sensor FDO 925, WTW, Poland) before and after the trials were 8.95 ± 0.04 and 8.85 ± 0.08 mg/l (mean ± SD), respectively, and did not differ among the experimental treatments.

Data analysis

We used Noldus Ethovision XT 10.1 software to quantify mussel behaviour on the basis of the recorded videos. Every second, we determined the position of the colour mark located on the movable mussel valve, relative to a constant point fixed on the other valve. Then, each valve position was expressed as the percentage of the maximum valve gaping observed for a given individual. We found no significant differences among the treatments in the maximum valve gaping (in mm) observed for each individual, showing that they all exhibited their maximum gaping at some time during the exposure. Moreover, we found no correlation between maximum valve gaping and mussel size (within the range used in the experiments). This justifies the use of the percentage gaping as the optimum response variable. The obtained dataset was further processed with a custom-made application written in Visual Basic for Microsoft Excel to detect gradual changes in valve position (opening and closing movements) and stable periods spent at particular valve gaping ranges, as well as their parameters (rate, amplitude, duration) during the experiment. We considered two valve movements as separate when they were separated by a stable period lasting for at least 5 s. We examined the following response variables: (1) Average valve gaping during the experiment; (2) Percentage of time spent by mussels with: (i) widely open valves (> 80% of the maximum valve gaping) (ii) narrowly open valves (< 20%) and (iii) totally closed valves (for better understanding of the average valve gaping value); (3) Number of valve opening movements. Visual inspection of the recordings indicated that mussels did not extend their siphons at valve gaping below 20%. Such a detailed approach can potentially lead to detection of subtle mussel reactions to environmental factors, undetectable with methods using only binary valve states (closed/open shell). All these parameters were calculated separately for the first and second hour of the experiment in order to reflect the potential impact of time on mussel responses to experimental factors.

We applied a General Linear Model analysis to test the effect of experimental treatments on the average valve gaping and percentages of time spent by mussels in particular gaping ranges. The models included two fixed factors: treatment (7 levels: various predation or gammarid cues and control) and experiment duration (repeated measures factor: hour 1 and 2) as well as their interaction. Normality and homoscedasticity were checked with Shapiro–Wilk and Levene tests, respectively, and percentage time data were log-transformed to meet these assumptions. A Tukey HSD test was used as a post hoc procedure for significant main effects of treatment and a sequential Bonferroni-corrected Fisher LSD test was applied for pairwise comparisons when an interaction was significant.

Numbers of valve opening movements were compared using a Generalized Linear Model (Poisson distribution, log-link function) including treatment, time (repeated measures) and their interaction. Sequential Bonferroni-corrected pairwise contrasts were used as a post hoc procedure.

The analyses were carried out using SPSS 25.0 statistical package (IBM Inc.).

Results

Average valve gaping during the experiment varied from 2 to 72% (Fig. 2) and significantly depended on experiment duration (valve gaping in the second hour of the experiment was by 10% higher than in the first hour) and on the cues (General Linear Model: Table 1A). Significantly lower valve gaping was observed in the presence of gammarids (2–3%) and the alarm substance alone (39–43%) relative to the control treatment (53–72%).

Fig. 2
figure 2

Average valve gaping of zebra mussels responding to the presence and diet of predators (fish), conspecific alarm substance (present: +, absent: −) and gammarid (D. villosus) cues. Presented values are means predicted for significant effects by the General Linear Model (± 95% confidence intervals). Treatments labelled with the same letter above the bar did not differ from one another with regard to the average valve gaping (within each experimental hour) (see Supplementary Table S1 for the full results of the post hoc analysis). N indicates the number of replicates

Table 1 Analyses of the zebra mussel valve movement parameters in response to the presence and diet of fish, conspecific alarm substance and gammarid (D. villosus) cues

The presence of gammarids, fish scents and/or alarm signals significantly affected the time spent by mussels with totally closed, narrowly open (0–20%) or widely open (> 80%) valves (General Linear Model: Table 1B–D). Time spent by mussels with totally closed valves varied depending on experiment duration (a significant treatment x time interaction). The highest total closure times were observed in the presence of gammarids (34–48% of the test duration), as well as, to a lesser extent and only during the first hour of the test, in the presence of the alarm substance alone (2.5%) and in the control treatment (1.8%) (Fig. 3A). The presence of fish scents (both diets) together with the alarm substance reduced the time spent with totally closed valves (0.1–0.2%) compared to the treatments with both these stimuli applied separately (0.5–2.5%).

Fig. 3
figure 3

Percentage time spent by zebra mussels at particular gaping ranges during exposure to the presence and diet of predators (fish), conspecific alarm substance (present: +, absent: −) and gammarid (D. villosus) cues. Presented values are means predicted for significant effects by the General Linear Model (± 95% confidence intervals). Treatments labelled with the same letter above the bar did not differ from one another with regard to the time spent at the particular valve gaping range (within each experimental hour, if separated) (see Supplementary Tables S2–S4 for the full results of the post hoc analyses)

Time spent by mussels with narrowly open valves (< 20%) depended on treatment only (General Linear Model: Table 1C). Mussels were narrowly open for a longer time in the presence of gammarids (95% of test duration) and the alarm substance alone (12%) (Fig. 3B). The shortest times spent with narrowly open valves were exhibited by mussels exposed to the fish scents (both diets) mixed with the alarm substance (1.7–3.1%). Values observed in these treatments differed significantly from the corresponding treatments with the alarm substance absent (4.8–6.0%).

Time spent with widely open valves (> 80%) depended on treatment only (General Linear Model: Table 1D) and was markedly lower in the presence of gammarids (0.1%) and the alarm substance alone (12%) (Fig. 3C). Moreover, this parameter was significantly lower in the presence of the alarm substance and the scent of fish fed with mussels (21%) than in the control treatment (38%).

The number of valve opening movements varied strongly among individuals, ranging from 1 to 816 per h. This parameter significantly depended on experimental treatment, but not on duration of the test (Generalized Linear Model: Table 1E). The mussels tested in the presence of gammarids (232 movements/h), as well as those exposed to the mixture of the scent of fish fed with mussels and the alarm substance (179 movements/h) were generally more active than in the other treatments (35–71 movements/h) (Fig. 4).

Fig. 4
figure 4

Numbers of valve opening movements exhibited by zebra mussels responding to the presence and diet of predators (fish), conspecific alarm substance (present: +, absent: −) and gammarid (D. villosus) cues. Presented values are means predicted for significant effects by the Generalized Linear Model (± 95% confidence intervals). Treatments labelled with the same letter above the bar did not differ from one another (see Supplementary Table S5 for the full results of the post hoc analysis)

Discussion

The most influential factors in our study turned out to be the physical presence of D. villosus [which naturally occurs in D. polymorpha colonies (Devin et al., 2003)] and the alarm substance excreted by injured conspecifics. Mussel responses to these factors were of similar character; however, the effect of the mechanical stimulus was much greater than that of the chemical predation cue. D. polymorpha exposed to gammarids spent most of the time with totally closed or narrowly open valves (< 20%), which resulted in the very narrow average valve gaping during the entire experiment. Visual inspection of the video recordings revealed that these strong behavioural reactions were likely connected with the protection of siphons and other exposed soft tissues against mechanical irritation by D. villosus appendages. The longer-term impact of D. villosus on D. polymorpha was reported by Kobak et al. (2012), who observed that mussels in the presence of amphipods increased their attachment strength and reduced upward movement. Moreover, D. villosus is considered to be a voracious and aggressive carnivore (Kley & Maier, 2005; Bacela-Spychalska et al., 2013). These features are associated with its direct and indirect negative impact on other amphipods (Bij de Vaate et al., 2002; Devin et al., 2003; MacNeil et al., 2013; Jermacz et al., 2015) and even fish fry (Platvoet et al., 2009). However, it has not been demonstrated to attack D. polymorpha directly, except one observation of gammarids feeding on mussel byssal threads (Platvoet et al., 2009). Thus, it is unlikely that D. villosus could pose a direct predatory threat to mussels. Nevertheless, taking into account the results of the present study, we assume that a prolonged exposure to its high density may negatively affect D. polymorpha condition.

In contrast to D. villosus, the presence of the alarm substance in the environment definitely indicates a direct and immediate danger, i.e. the proximity of a foraging predator (Wisenden, 2015). In our study, mussels exposed to this cue significantly reduced their average valve gaping and time spent with widely open valves (> 80%). This behaviour does not defend them during a direct attack of a shell-crushing predator, but can prevent detection by limiting the amount of infochemicals released from the exposed mantle surface and siphonal currents generated during filtration (Weissburg et al., 2002; Smee & Weissburg, 2006; Antoł et al., 2018). Similarly to our results, Robson et al. (2007, 2010) revealed that another bivalve species, Mytilus edulis Linnaeus, 1758 in the presence of predation risk (alarm cue) demonstrated smaller gape angles. Moreover, in the presence of the alarm cue, mytilids closed their valves more rapidly and became less responsive to food. Strong behavioural responses to alarm cues have also been described for many freshwater animals (Wisenden & Millard, 2001; Wisenden et al., 2001; Dalesman et al., 2006; Smee & Weissburg, 2006), indicating the importance of this factor in shaping animal behaviour. Predator foraging activity can be triggered by chemical signals from metabolically active mussels (Czarnołęski et al., 2011), which further supports the protective value of valve gaping reduction.

The reduced valve gaping of D. polymorpha exposed to the alarm cues, found in our study, corroborates previous observations, revealing that byssate mussels during the first few hours of exposure to predation cues reduce their attachment (Reimer & Tedengren, 1997) travelled distances (Toomey et al., 2002; Czarnołęski et al., 2010) and respiration rates (Antoł et al., 2018). All these responses lead to decreased probability of detection of mussels by predators (Antoł et al., 2018). However, after the initial activity reduction, mussels exposed to predation cues were found to relocate more than in the control treatment (Kobak & Ryńska, 2014) and attach more strongly to substratum (Reimer & Tedengren, 1997; Kobak et al., 2010), indicating the importance of time factor for shaping animal responses to danger cues.

For most of the analysed valve movement parameters we did not reveal any specific effects of the predator (the roach R. rutilus) scent itself, separated from conspecific alarm cues, regardless of the differences in the roach diet. In a previous experimental study, Kobak et al. (2010) demonstrated that mussels were capable of recognizing the scent of roach (fed with neutral chironomid diet) and in response to this stimulus attached more strongly to the substratum, though the effect appeared after 6 days of exposure. These findings show that mussels can adjust their anti-predator strategy depending on the exposure time and proximity of the danger. Their immediate responses to direct dangers (currently hunting predators indicated by the presence of the conspecific alarm cue) involve activity decrease aimed at hiding the prey presence from the predator (Reimer & Tedengren, 1997; Czarnołęski et al., 2010; Antoł et al., 2018). When a danger is indirect (a non-foraging predator releasing only kairomones) and/or exposure lasts for a longer time, different mechanisms become involved, increasing the mussel security through stronger attachment (Reimer & Tedengren, 1997), aggregation (Kobak et al., 2010) and/or development of thicker shells (Czarnołęski et al., 2006; Naddafi & Rudstam, 2014). Changes in valve movements seem to be the first behavioural reaction exhibited immediately after detecting predatory cues, but only in the case of a direct threat (as the alarm substance in our experiments). The alarm cue in the direct vicinity may be treated as a sign of an immediate danger by mussels, with no time to employ more sophisticated and time-consuming defence mechanisms. On the other hand, valve gaping reduction in response to indirect and/or long-term predation danger would be too costly, limiting respiration and feeding (Jørgensen et al., 1988), whereas the presence of some numbers of predators in the environment is a natural situation in the wild.

In our study, mussels exposed to the alarm cues spent more time with totally closed or only narrowly open valves (< 20%) which was probably insufficient for effective filtration (Jørgensen et al., 1988; Newell et al., 1998; Robson et al., 2010), as at this degree of valve opening they were unable to extend their siphons (personal observation). This narrow valve opening could be related to a compromise between hiding and sustaining respiration (impossible during the complete closure) or to periodic “testing the external environment” in a stress situation. The latter hypothesis is also supported by another reaction of mussels exposed to alarm cues in our study: the increased number of valve opening movements. This seems useful, as completely closed mussels have impaired sensory contact with the external environment and, being unable to sustain filter-feeding and aerobic respiration, bear severe costs of non-consumptive predation effects. Thus, although D. polymorpha can stay closed even for several days protecting themselves against unfavourable environmental conditions (Rajagopal et al., 2010), it is beneficial if they can quickly detect a positive change in their environment and return to their normal activity as soon as possible.

Another interesting phenomenon observed in our study concerns the reactions of mussels to the scent of fish mixed with the alarm substance and/or dietary alarm cue. We supposed these separate predation cues to act synergistically and elicit stronger mussel responses than each of them applied separately (Dalesman et al., 2006). In contrast to this expectation, the compound stimulus (i.e. the conspecific alarm substance combined with predator scent) made mussels spend less time with the totally closed or narrowly open valves compared to the treatments with separate alarm cue and fish scents. On the other hand, mussels exposed to the mixture of the alarm cue and the scent of a predator fed with conspecifics still showed the increased number of valve movements, similar to that exhibited by the individuals exposed to the alarm cue alone. Nevertheless, the response of mussels to the mixed predation cue was clearly weaker than that observed in the presence of the alarm substance alone and actually more similar to the results of the control treatment. Taking into account the fact that we always used the same amount of the alarm cue, the change in its effect on mussel behaviour must have been caused by the presence of fish infochemicals. Perhaps, the predator might be able to mask the components of alarm cues (including those originating from its faeces containing the remnants of digested mussels). It should be noted that, in the first hour of experiment, the control mussels tended to be totally closed for a longer time than those exposed to the fish scents. This might point to the fact that they were still adapting to the experimental conditions, whereas this effect did not occur in the presence of fish scents, potentially supporting our hypothesis on their masking effect on stressors influencing mussel behaviour. Anyway, further experimental evidence is needed to disentangle this observation. A similar phenomenon was suggested by Wisenden (2015) but only for dietary alarm cues (postingestion cues released by the predator, the northern pike Esox lucius) of the ruffe (Gymnocephalus cernuus) (Maniak et al., 2000). Similarly, Feminella & Hawkins (1994) observed that tadpoles of the tailed frog (Ascaphus truei) were unable to detect the presence of their predator, the shorthead sculpin (Cottus confusus) fed with conspecific tadpoles, whereas other predator species were recognized. It is not known, however, whether the tadpoles can respond to the scent of the conspecific alarm cues alone. Thus, to our knowledge, our work is the first to suggest an interaction between predator infochemicals and alarm cues released by wounded prey resulting in the weakening of the prey response. Definitely, this phenomenon deserves further research to explain its mechanisms.

Conclusion

We have shown that biotic environmental factors, such as predation alarm cues and mechanical irritation by mobile dwellers of a mussel bed, may significantly affect D. polymorpha valve movements. This points to potential negative non-consumptive effects on mussels, disrupting their normal filtration and respiration activity. Due to the common occurrence of both stimuli in the wild, we can expect that they may have a real influence on the functioning of mussel colonies, including their filtration (which varies with the degree of valve opening, Jørgensen et al., 1988; Newell et al., 1998; Saurel et al., 2007), being one of the most important aspects of their environmental impact. The decrease in filtration efficiency in the presence of predators was demonstrated by Naddafi et al. (2007). Thus, colony filtration rates may be overestimated if these factors are not taken into account.

Our study together with the existing literature (Naddafi et al., 2007; Shin et al., 2008; Cheung et al., 2009; Czarnołęski et al., 2010; Kobak et al., 2010; Naddafi & Rudstam, 2013; Antoł et al., 2018) shows that sessile organisms are capable of fine-tuning their responses to specific situations involving variable predation pressure, taking multiple factors, such as the vicinity and condition of a predator as well as duration of exposure into account. Although unable to escape from environmental dangers and having limited possibilities of selecting their location, they can use a variety of behavioural responses to avoid detection by predators and/or to make their handling more difficult.

Additionally, our findings show that in behavioural studies on mussel valve movements special attention should be paid to subtle, non-binary valve reactions, which can reveal responses invisible when only two valve states (open/closed) are considered. Thus, our study may also be important for the development of early warning systems detecting environmental pollution on the basis of mussel valve movement patterns (Kramer et al., 1989; Borcherding, 2006; Robson et al., 2007; Redmond et al., 2017). Such non-target factors as predation pressure should be taken into account when calibrating these systems to avoid false alarms.

Several questions raised by our present study still await answers to be given by future research, the most important being valve movement responses to the studied factors over a longer time scale (e.g. after several days of exposure) and the mechanism behind the observed reduction in the effect of the alarm cue in the presence of fish.

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Acknowledgements

Our study was supported by a grant of the Polish National Science Centre No. 2015/17/N/NZ8/01653.

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Dzierżyńska-Białończyk, A., Jermacz, Ł., Zielska, J. et al. What scares a mussel? Changes in valve movement pattern as an immediate response of a byssate bivalve to biotic factors. Hydrobiologia 841, 65–77 (2019). https://doi.org/10.1007/s10750-019-04007-0

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Keywords

  • Valve gaping
  • Dreissena polymorpha
  • Predation cues
  • Activity
  • Induced defence