Cryptic lineages and hybridization of the predaceous chub Parazacco spilurus (Actinopterygii, Cypriniformes, Xenocyprididae) in Hong Kong
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Fine-scale genetic studies are essential for understanding population connectivity and formulating appropriate conservation measures for freshwater inhabitants. Despite their significance, such studies remain relatively limited in South China and Asia in general. We examined the genetic structure of the predaceous chub Parazacco spilurus in Hong Kong by incorporating genetic data from both mitochondrial (control region and cytochrome b oxidase gene) and nuclear (recombination-activating gene 1) DNA markers. We identified two highly divergent lineages having discrete distribution ranges with limited overlap near two reservoirs located in north-eastern (Plover Cove Reservoir) and south-eastern (Tai Tam Tuk Reservoir) Hong Kong. Each lineage is further divided into sublineages with geographical association. As such, gene flow is highly restricted at both sublineage and population levels. Despite the presence of sublineages, inter-lineage divergence is far greater than that divergence within lineage. It also exceeds the species divergence in closely related cyprinid genera, suggesting that the two lineages recovered potentially represent two distinct species. Yet genetic analyses based on the nuclear marker indicated a low degree of hybridization between the lineages at two closely situated localities. This study provides insights on the possible direction of conservation plans for P. spilurus in Hong Kong and South China.
KeywordsCryptic diversity Freshwater fish Population genetics
This work was supported by the two research grants (33/2009 and 28/2013) from the Environment and Conservation Fund, Hong Kong SAR, China. We thank B. T. S. Bui, K. Y. Lau, C. P. Li, and K. C. Cheung (The Chinese University of Hong Kong) for field collection and technical assistance. Thanks also go to the staff of the Agriculture, Fisheries and Conservation Department, Hong Kong SAR Government for their valuable advice on field sites for sampling. The authors offer their appreciation to K. Y. Ma (The Chinese University of Hong Kong) and D. Dudgeon (The University of Hong Kong) for their inspiring discussion.
This work was supported by the two research grants (33/2009 and 28/2013) from the Environment and Conservation Fund, Hong Kong SAR, China.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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