, Volume 819, Issue 1, pp 243–257 | Cite as

Species assignment and population genetic studies of Gran Paraná pejerrey (Odontesthes sp., Atheriniformes, Atherinopsidae) from La Plata Basin in South America

  • Gabriela Vanina Villanova
  • Manuel Vera
  • Florencia Brancolini
  • Juan Díaz
  • Paulino Martinez
  • Silvia Eda Arranz
Primary Research Paper


Pejerrey is the common name given to Odontesthes species from South America. Every year, individuals of pejerrey called “Gran (Big) Paraná” appear during the low-temperature season at rivers in the southern section of the La Plata Basin. Gran Paraná fishes are highly appreciated for fishing, and present some biological features different from other well-characterized Odontesthes fish, such as bigger size and migratory behavior. Regulations for the management of pejerrey fisheries within La Plata Basin have not been implemented yet. The aims of the present work were to characterize the Gran Paraná pejerrey species by molecular methods and carry out the first population genetic study of Gran Parana pejerrey from the La Plata Basin. All Gran Paraná specimens were classified as O. bonariensis, based on both morphology and microsatellite loci. Genetic differentiation was observed between Gran Paraná pejerrey and O. bonariensis pejerrey sampled at Chascomús lagoon. In addition, temporal genetic differentiation was observed, suggesting the presence of different cohorts migrating along the basin. The knowledge of population dynamics and differentiation will contribute to figure out fisheries models and to design protective areas for this species under commercial exploitation.


Molecular markers River Estuary Genetics Sustainability Fish Odontesthes bonariensis 



We would like to thank Alexis Grimberg, Julián Aguilar, and Diego Añaño and team for fishing assistance, Darío Colautti for the kind donation of samples, Victoria Posner for reading the manuscript, and anonymous reviewers that helped to improve our work. This work was supported by the National Agency for the Promotion of Science and Technology from Argentina (ANPCyT) and the Government of Santa Fe province [Grant Numbers: PID 020-2013 and PICT 0510-2011]; FB is a Ph. D student, JD is a postdoctoral fellow, and GVV is member of the researcher carrier from the National Council of Research from Argentina.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Supplementary material

10750_2018_3643_MOESM1_ESM.eps (20.9 mb)
Figure S1. Genetic analyses using CR mtDNA marker. (A) Maximum-Likelihood tree. The evolutionary history was inferred by using the Maximum-Likelihood method based on the Tamura 3-parameter model (Tamura, 1992) (α = 0.1343; ts/tv: 4.87). The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. (B) Median Joining Network. Numbers correspond to CRs haplotypes numbers. The size of circles is proportional to the number of individuals that share a particular haplotype. Median vectors are indicated as red spots. Each color represents a sample site or species previously described, and it is indicated at the color reference box. Branches length is proportional to the number of differences between haplotypes. (EPS 21443 kb)
10750_2018_3643_MOESM2_ESM.eps (7.5 mb)
Figure S2. Factorial correspondence analysis (FCA) plot. Genetic relationships between pejerrey individuals collected from PR, UR, iRLP, cRLP and CHL, at different years are shown in a 3D FCA plot. (EPS 7695 kb)
10750_2018_3643_MOESM3_ESM.docx (36 kb)
Supplementary Table 1 (DOCX 36 kb)
10750_2018_3643_MOESM4_ESM.docx (24 kb)
Supplementary Table 2 (DOCX 23 kb)
10750_2018_3643_MOESM5_ESM.docx (16 kb)
Supplementary Table 3 (DOCX 16 kb)
10750_2018_3643_MOESM6_ESM.docx (14 kb)
Supplementary Table 4 (DOCX 13 kb)
10750_2018_3643_MOESM7_ESM.docx (12 kb)
Supplementary Table 5 (DOCX 12 kb)


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Gabriela Vanina Villanova
    • 1
  • Manuel Vera
    • 2
  • Florencia Brancolini
    • 3
  • Juan Díaz
    • 1
  • Paulino Martinez
    • 2
  • Silvia Eda Arranz
    • 1
  1. 1.Laboratorio de Biotecnología AcuáticaFacultad de Ciencias Bioquímicas y Farmacéuticas – Universidad Nacional de Rosario- Centro Científico, Tecnológico y Educativo Acuario del Río ParanáRosarioArgentina
  2. 2.Department of Zoology, Genetics and Physical Anthropology, Faculty of VeterinaryUniversity of Santiago de CompostelaLugoSpain
  3. 3.Instituto de Investigación e Ingeniería AmbientalUniversidad Nacional de San Martín, CONICETSan MartinArgentina

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