Species validation and cryptic diversity in the Geophagus brasiliensis Quoy & Gaimard, 1824 complex (Teleostei, Cichlidae) from Brazilian coastal basins as revealed by DNA analyses

  • Cássio Ribeiro Souza
  • Paulo Roberto Antunes de Mello Affonso
  • Jamille de Araújo Bitencourt
  • Iracilda Sampaio
  • Paulo Luiz Souza Carneiro
Primary Research Paper
  • 25 Downloads

Abstract

Many allopatric populations of the pearl cichlid Geophagus brasiliensis show remarkable morphological, chromosomal, and DNA sequence divergence. As a result, Geophagus brasiliensis is regarded as a species complex, combining recent descriptions of new species and several uncertainties about their actual richness. In the present work, we evaluated the number of evolutionary units in this fish complex along hydrographic basins in northeastern Brazil by using the DNA barcode, species delimitation algorithms and phylogenetic analyses. A total of 14 molecular operational taxonomic units (MOTUS) were identified with high support values and mean differentiation within and among groups of 0.4 and 12.7%, respectively. The DNA sequences supported Geophagus itapicuruensis as a valid taxon, besides indicating likely undescribed species. Moreover, the evolutionary units in coastal basins of useful to infer the evolutionary units in a cryptic and widespread fish group from the Neotropical region. Finally, the undescribed taxa in coastal basins of northeastern Brazil were clearly differentiated from Geophagus brasiliensis sensu stricto. The present data highlight the importance of conserving regional ichthyofauna since unique evolutionary lineages are potentially threatened by environmental degradation, invasive species and construction of dams.

Keywords

Barcode Cichlids Conservation Diversity Systematics 

Notes

Acknowledgements

The authors would like to thank FAPESB (RED0009/2013), “Rede de DNA barcoding da ictiofauna do Brasil” (MCT/CNPq/FNDCT 50/2010), and CNPq (610013/2011-4) for the financial support.

Supplementary material

10750_2017_3482_MOESM1_ESM.doc (60 kb)
Supplementary material 1 (DOC 60 kb)
10750_2017_3482_MOESM2_ESM.tif (6.8 mb)
Online Resource 2 PhyloMap PTP tree based on COI sequences of the samples of Geophagus brasiliensis complex from the present study and related species available in BOLD. The circles in the graph represent the taxa and species are indicated by distinct colors. The horizontal and vertical axis explained 65.80% and 13.56% of total variation, respectively. The thicker lines indicate that the length of branches in the original species tree is longer than those shown in the graph. Supplementary material 2 (TIFF 6962 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  • Cássio Ribeiro Souza
    • 1
  • Paulo Roberto Antunes de Mello Affonso
    • 1
  • Jamille de Araújo Bitencourt
    • 1
  • Iracilda Sampaio
    • 2
  • Paulo Luiz Souza Carneiro
    • 1
  1. 1.Department of Biological SciencesUniversidade Estadual do Sudoeste da BahiaJequiéBrazil
  2. 2.Institute of Coastal StudiesUniversidade Federal do ParáBragançaBrazil

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