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DNA barcoding as a useful tool for identifying non-native species of freshwater ichthyoplankton in the neotropics

  • INVASIVE SPECIES II
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Abstract

The objective of our study was to evaluate the efficacy of DNA barcoding as a tool to identify non-native fish in their early stages of life. Specimens were collected from 14 sites in the lower and middle regions of the Paranapanema River—the main tributary of the Upper Paraná River basin in southern Brazil. Over three reproductive periods from 2012 and 2015, 75 eggs and 170 larvae from 13 non-native fish species were identified. The most abundant species were Plagioscion squamosissimus (75 specimens) and Serrasalmus marginatus (68 specimens). The highest abundance was found near the Rosana hydroelectric plant reservoir. The removal of the natural physical barrier is the main reason that fish are introduced into the Paranapanema River basin with higher occurrence in the Rosana reservoir. P. squamosissimus was found in all reservoirs. DNA barcoding is a useful tool for identifying non-native fish during their reproductive phase. Furthermore, recording the presence of potentially invasive species in their early development stages may provide information useful for the rapid and effective control.

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Authors and Affiliations

  1. Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, PR 445 km 380, Londrina, PR, CEP: 86057-970, Brazil

    Fernanda S. Almeida, Wilson Frantine-Silva & Same C. Lima

  2. Departamento de Biologia Animal e Vegetal, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, PR 445 km 380, Londrina, PR, 86057-970, Brazil

    Diego A. Z. Garcia & Mário L. Orsi

Authors
  1. Fernanda S. Almeida
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  2. Wilson Frantine-Silva
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  3. Same C. Lima
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  4. Diego A. Z. Garcia
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  5. Mário L. Orsi
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Corresponding author

Correspondence to Fernanda S. Almeida.

Additional information

Guest editors: John E. Havel, Sidinei M. Thomaz, Lee B. Kats, Katya E. Kovalenko & Luciano N. Santos / Aquatic Invasive Species II

Data Accessibility

Bold dataset for non-native species: http://www.boldsystems.org/index.php/Public_SearchTerms?query=DS-IFEL. BOLD projects: container project FELPR (Projects: PDCAI, accessions PDCAI001-13 to PDCAI134-13; PDCAP, accessions PDCAP001-14 to PDCAP287-14; PDCII accessions PDCII115-14). BOLD annexed project FUPR, accessions: BAST525-12, FUPR001-09 to FUPR1468-10. BOLD projects: PRP, Fish eggs and larvae of Paranapanema river (Accessions PRP001-16 to PRP960-16).

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Fig. S1

BOLD TaxonID Tree for non-native fish species identified by DNA barcoding data along the lower–middle Paranapanema River (PDF 10 kb)

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Almeida, F.S., Frantine-Silva, W., Lima, S.C. et al. DNA barcoding as a useful tool for identifying non-native species of freshwater ichthyoplankton in the neotropics. Hydrobiologia 817, 111–119 (2018). https://doi.org/10.1007/s10750-017-3443-5

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  • DOI: https://doi.org/10.1007/s10750-017-3443-5

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