Diversity and the environmental drivers of spatial variation in Bacteria and micro-Eukarya communities from the Hawaiian anchialine ecosystem

Abstract

Little is currently known regarding microbial community structure, and the environmental factors influencing it, within the anchialine ecosystem, defined as near-shore, land-locked water bodies with subsurface connections to the ocean and groundwater aquifer. The Hawaiian Archipelago is home to numerous anchialine habitats, with some on the islands of Maui and Hawaii harboring unique, laminated orange cyanobacterial–bacterial crusts that independently assembled in relatively young basalt fields. Here, benthic and water column bacterial and micro-eukaryotic communities from nine anchialine habitats on Oahu, Maui, and Hawaii were surveyed using high-throughput amplicon sequencing of the V6 (Bacteria-specific) and V9 (Eukarya-biased) hypervariable regions of the 16S- and 18S-rDNA genes, respectively. While benthic communities from habitats with cyanobacterial–bacterial crusts were more similar to each other than to ones lacking it on the same island, each habitat had distinct benthic and water column microbial communities. Analyses of the survey data in the context of environmental factors identified salinity, site, aquifer, and watershed as having the highest explanatory power for the observed variation in microbial diversity and community structure, with lesser drivers being annual rainfall, longitude, ammonium, and dissolved organic carbon. Our results epitomize the abiotic and biotic uniqueness characteristic of individual habitats comprising the Hawaiian anchialine ecosystem.

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References

  1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers & D. J. Lipman, 1990. Basic local alignment search tool. Journal of Molecular Biology 215: 403–410.

    CAS  Article  PubMed  Google Scholar 

  2. Amaral-Zettler, L. A., E. A. McCliment, H. W. Ducklow & S. M. Huse, 2009. A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes. PLoS ONE 4: e6372.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Bailey-Brock, J. H. & R. E. Brock, 1993. Feeding, reproduction, and sense organs of the Hawaiian anchialine shrimp Halocaridina rubra (Atyidae). Pacific Science 47: 338–355.

    Google Scholar 

  4. Bender, J. & P. Phillips, 2004. Microbial mats for multiple applications in aquaculture and bioremediation. Bioresource Technology 94: 229–238.

    CAS  Article  PubMed  Google Scholar 

  5. Bender, J., R. F. Lee & P. Phillips, 1995. Uptake and transformation of metals and metalloids by microbial mats and their use in bioremediation. Journal of Industrial Microbiology 14: 113–118.

    CAS  Article  Google Scholar 

  6. Benson, D. A., I. Karsch-Mizrachi, D. J. Lipman, J. Ostell & E. W. Sayers, 2009. GenBank. Nucleic Acids Research 37: D26–D31.

    CAS  Article  PubMed  Google Scholar 

  7. Bishop, R. E., B. Kakuk & J. J. Torres, 2004. Life in the hypoxic and anoxic zones: metabolism and proximate composition of Caribbean troglobitic crustaceans with observations on the water chemistry of two anchialine caves. Journal of Crustacean Biology 24: 379–392.

    Article  Google Scholar 

  8. Bokulich, N. A., S. Subramanian, J. J. Faith, D. Gevers, J. I. Gordon, R. Knight, D. A. Mills & J. G. Caporaso, 2013. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nature Methods 10: 57–59.

    CAS  Article  PubMed  Google Scholar 

  9. Bray, J. R. & J. T. Curtis, 1957. An ordination of the upland forest communities of southern Wisconsin. Ecological Monographs 27: 325–349.

    Article  Google Scholar 

  10. Brock, R. E. & J. H. Bailey-Brock, 1998. An unique anchialine pool in the Hawaiian Islands. International Review of Hydrobiology 83: 65–75.

    CAS  Article  Google Scholar 

  11. Brock, R. E., J. E. Norris, D. A. Ziemann & M. T. Lee, 1987. Characteristics of water quality in anchialine ponds of Kona, Hawaii Coast. Pacific Science 41: 200–208.

    CAS  Google Scholar 

  12. Caporaso, J. G., K. Bittinger, F. D. Bushman, T. Z. DeSantis, G. L. Andersen & R. Knight, 2010a. PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26: 266–267.

    CAS  Article  PubMed  Google Scholar 

  13. Caporaso, J. G., J. Kuczynski, J. Stombaugh, K. Bittinger, F. D. Bushman, E. K. Costello, N. Fierer, A. G. Pena, J. K. Goodrich, J. I. Gordon, G. A. Huttley, S. T. Kelley, D. Knights, J. E. Koenig, R. E. Ley, C. A. Lozupone, D. McDonald, B. D. Muegge, M. Pirrung, J. Reeder, J. R. Sevinsky, P. J. Turnbaugh, W. A. Walters, J. Widmann, T. Yatsunenko, J. Zaneveld & R. Knight, 2010b. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7: 335–336.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. Chao, A., 1984. Nonparametric estimation of the number of classes in a population. Scandinavian Journal of statistics 11: 265–270.

    Google Scholar 

  15. Chien, A., D. B. Edgar & J. M. Trela, 1976. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. Journal of Bacteriology 127: 1550–1557.

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Clarke, K. R., 1993. Non-parametric multivariate analyses of changes in community structure. Australian Journal of Ecology 18: 117–143.

    Article  Google Scholar 

  17. Clarke, K. R. & M. Ainsworth, 1993. A method of linking multivariate community. Marine ecology progress series 92: 205–219.

    Article  Google Scholar 

  18. Cockell, C. S. & H. L. Jones, 2009. Advancing the case for microbial conservation. Oryx 43: 520–526.

    Article  Google Scholar 

  19. Colwell, R. R., 1997. Microbial diversity: the importance of exploration and conservation. Journal of Industrial Microbiology & Biotechnology 18: 302.

    CAS  Article  Google Scholar 

  20. Craft, J. D., A. D. Russ, M. N. Yamamoto, T. Y. Iwai, S. Hau, J. Kahiapo, C. T. Chong, S. Ziegler-Chong, C. Muir, Y. Fujita, D. A. Polhemus, R. A. Kinzie & S. R. Santos, 2008. Islands under islands : the phylogeography and evolution of Halocaridina rubra Holthuis, 1963 (Crustacean: Decapoda: Atyidae) in the Hawaiian Archipelago. Limnology and oceanography 53: 675–689.

    Article  Google Scholar 

  21. Dalton, C. M., A. Mokiao-Lee, T. S. Sakihara, M. G. Weber, C. A. Roco, Z. Han, B. Dudley, R. A. MacKenzie & N. G. Hairston, 2013. Density- and trait-mediated top–down effects modify bottom–up control of a highly endemic tropical aquatic food web. Oikos 122: 790–800.

    Article  Google Scholar 

  22. De Mendiburu, F., 2015. Agricolae: Statistical procedures for agricultural research. R package version 1.2-3: 1–6.

  23. DeSantis, T. Z., P. Hugenholtz, N. Larsen, M. Rojas, E. L. Brodie, K. Keller, T. Huber, D. Dalevi, P. Hu & G. L. Andersen, 2006. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Applied and Environmental Microbiology 72: 5069–5072.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  24. Donachie, S. P., S. Hou, K. S. Lee, C. W. Riley, A. Pikina, C. Belisle, S. Kempe, T. S. Gregory, A. Bossuyt, J. Boerema, J. Liu, T. A. Freitas, A. Malahoff & M. Alam, 2004. The Hawaiian Archipelago: a microbial diversity hotspot. Microbial Ecology 48: 509–520.

    CAS  Article  PubMed  Google Scholar 

  25. Dunfield, K. E. & G. M. King, 2004. Molecular analysis of carbon monoxide-oxidizing bacteria associated with recent Hawaiian volcanic deposits. Applied and Environmental Microbiology 70: 4242–4248.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  26. Edgar, R. C., 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26: 2460–2461.

    CAS  Article  PubMed  Google Scholar 

  27. Garman, K., H. Rubelmann, D. Karlen, T. Wu & J. Garey, 2011. Comparison of an inactive submarine spring with an active nearshore anchialine spring in Florida. Hydrobiologia 677: 65–87.

    CAS  Article  Google Scholar 

  28. Giambelluca, T. W., X. Shuai, M. L. Barnes, R. J. Alliss, R. J. Longman, T. Miura, Q. Chen, A. G. Frazier, R. G. Mudd, & L. Cuo, 2014. Evapotranspiration of Hawai ‘i. Final report submitted to the US Army Corps of Engineers – Honolulu District, and the Commission on Water Resource Management, State of Hawai ‘i .

  29. Gloor, G. B., R. Hummelen, J. M. Macklaim, R. J. Dickson, A. D. Fernandes, R. MacPhee & G. Reid, 2010. Microbiome profiling by Illumina sequencing of combinatorial sequence-tagged PCR products. PLoS ONE 5: e15406.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Gomez-Alvarez, V., G. M. King & K. Nüsslein, 2007. Comparative bacterial diversity in recent Hawaiian volcanic deposits of different ages. FEMS Microbiology Ecology 60: 60–73.

    CAS  Article  PubMed  Google Scholar 

  31. Gonzalez, B., T. Iliffe, J. Macalady, I. Schaperdoth & B. Kakuk, 2011. Microbial hotspots in anchialine blue holes: initial discoveries from the Bahamas. Hydrobiologia 677: 149–156.

    CAS  Article  Google Scholar 

  32. Gordon, A., & G. J. Hannon, 2010. Fastx-toolkit. FASTQ/A short-reads preprocessing tools (unpublished). http://hannonlab.cshl.edu/fastx_toolkit.

  33. Griffith, G. W., 2012. Do we need a global strategy for microbial conservation? Trends in Ecology & Evolution 27: 1–2.

    Article  Google Scholar 

  34. Havird, J., J. Weeks, S. Hau & S. Santos, 2013. Invasive fishes in the Hawaiian anchialine ecosystem: investigating potential predator avoidance by endemic organisms. Hydrobiologia 716: 189–201.

    CAS  Article  Google Scholar 

  35. Havird, J. C., S. R. Santos & R. P. Henry, 2014. Osmoregulation in the Hawaiian anchialine shrimp Halocaridina rubra (Crustacea: Atyidae): Expression of ion transporters, mitochondria-rich cell proliferation and hemolymph osmolality during salinity transfers. Journal of Experimental Biology 217: 2309–2320.

    Article  PubMed  Google Scholar 

  36. Holthuis, L. B., 1963. On red coloured shrimps (Decapoda, Caridea) from tropical land-locked saltwater pools. Zoologische Mededelingen 16: 261–279.

    Google Scholar 

  37. Holthuis, L. B., 1973. Caridean shrimps found in land-locked saltwater pools at four Indo-West Pacific localities (Sinai Peninsula, Funafuti Atoll, Maui and Hawaii Islands), with the description of one new genus and four new species. Zoologische Verhandelingen 128: 1–48.

    Google Scholar 

  38. Humphreys, W. F., 1999. Physico-chemical profile and energy fixation in Bundera Sinkhole, an anchialine remiped habitat in north-western Australia. Journal of the Royal Society of Western Australia 82: 89–98.

    Google Scholar 

  39. Humphreys, W., S. Tetu, L. Elbourne, M. Gillings, J. Seymour, J. Mitchell & I. Paulsen, 2012. Geochemical and microbial diversity of Bundera Sinkhole, an anchialine system in the eastern Indian ocean. Natura Croatica 21: 59–63.

    Google Scholar 

  40. Huse, S. M., D. M. Welch, H. G. Morrison & M. L. Sogin, 2010. Ironing out the wrinkles in the rare biosphere through improved OTU clustering. Environmental Microbiology 12: 1889–1898.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  41. Iliffe, T. M., 2002. Conservation of anchialine cave biodiversity. In Martin, J. B., C. M. Wicks, & I. D. Sasowsky (eds), Hydrogeology and Biology of Post-Paleozoic Carbonate Aquifers. Karst Waters Institute, Charles Town: 99–102.

  42. Ishino, S., & Y. Ishino, 2014. DNA polymerases as useful reagents for biotechnology – the history of developmental research in the field. Frontiers in Microbiology 5. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148896/.

  43. Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles 44: 223–270.

    Google Scholar 

  44. Kano, Y. & T. Kase, 2004. Genetic exchange between anchialine cave populations by means of larval dispersal: the case of a new gastropod species Neritilia cavernicola. Zoologica Scripta 33: 423–437.

    Article  Google Scholar 

  45. Konhauser, K. O., B. Jones, A.-L. Reysenbach & R. W. Renaut, 2003. Hot spring sinters: keys to understanding Earth’s earliest life forms. Canadian Journal of Earth Sciences 40: 1713–1724.

    Article  Google Scholar 

  46. Krstulović, N., M. Šolić, D. Šantić, J. Maršić-Lučić, M. Ordulj & S. Šestanović, 2013. Microbial community structure in two anchialine caves on Mljet Island (Adriatic Sea). Acta Adriatica 54: 183–198.

    Google Scholar 

  47. Kunin, V., A. Engelbrektson, H. Ochman & P. Hugenholtz, 2010. Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates. Environmental Microbiology 12: 118–123.

    CAS  Article  PubMed  Google Scholar 

  48. Langenheder, S., V. Kisand, J. Wikner & L. J. Tranvik, 2003. Salinity as a structuring factor for the composition and performance of bacterioplankton degrading riverine DOC. FEMS Microbiology Ecology 45: 189–202.

    CAS  Article  PubMed  Google Scholar 

  49. Maciolek, J. A., 1983. Distribution and biology of Indo-Pacific insular hypogeal shrimps. Bulletin of Marine Science 33: 606–618.

    Google Scholar 

  50. Maciolek, J. A. & R. E. Brock, 1974. Aquatic survey of the Kona coast ponds, Hawaii Island. U.S. Department of Commerce and Hawaii Cooperative Fishery Unit, Honolulu, HI.

    Google Scholar 

  51. Marrack, L., 2015. Modeling potential shifts in Hawaiian anchialine pool habitat and introduced fish distribution due to sea level rise. Estuaries Coasts. doi:10.1007/s12237-015-0025-5.

    Google Scholar 

  52. Marrack, L., S. Beavers & P. O’Grady, 2015. The relative importance of introduced fishes, habitat characteristics, and land use for endemic shrimp occurrence in brackish anchialine pool ecosystems. Hydrobiologia 758: 107–122.

    CAS  Article  Google Scholar 

  53. Masella, A. P., A. K. Bartram, J. M. Truszkowski, D. G. Brown & J. D. Neufeld, 2012. PANDAseq: paired-end assembler for Illumina sequences. BMC Bioinformatics 13: 31.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  54. McMurdie, P. J. & S. Holmes, 2013. phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE 8: e61217.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  55. Mejía-Ortíz, L. M., G. Yánez & M. López-Mejía, 2007. Echinoderms in an anchialine cave in Mexico. Marine Ecology 28: 31–34.

    Article  Google Scholar 

  56. Menning, D., L. M. Boop, E. D. Graham & J. R. Garey, 2014. Molecular analyses of microbial abundance and diversity in the water column of anchialine caves in Mallorca, Spain. International Journal of Speleology 43: 9.

    Article  Google Scholar 

  57. Nüsslein, K. & J. M. Tiedje, 1998. Characterization of the dominant and rare members of a young Hawaiian soil bacterial community with small-subunit ribosomal DNA amplified from DNA fractionated on the basis of its guanine and cytosine composition. Applied and Environmental Microbiology 64: 1283–1289.

    PubMed  PubMed Central  Google Scholar 

  58. Nüsslein, K. & J. M. Tiedje, 1999. Soil bacterial community shift correlated with change from forest to pasture vegetation in a tropical soil. Applied and Environmental Microbiology 65: 3622–3626.

    PubMed  PubMed Central  Google Scholar 

  59. Office of Planning: Hawaii Statewide GIS Program. http://planning.hawaii.gov/gis/.

  60. Oksanen, J., F. G. Blanchet, R. Kindt, P. Legendre, P. R. Minchin, R. B. O’Hara, G. L. Simpson, P. Solymos, M. H. H. Stevens, & H. Wagner, 2015. Vegan: Community ecology package. R package version 2.3-0. http://CRAN.R-project.org/package=vegan.

  61. Paniagua-Michel, J. & O. Garcia, 2003. Ex-situ bioremediation of shrimp culture effluent using constructed microbial mats. Aquacultural Engineering 28: 131–139.

    Article  Google Scholar 

  62. Pedrós-Alió, C., 2007. Dipping into the rare biosphere. Science 315: 192–193.

    Article  PubMed  Google Scholar 

  63. Phillips, P., J. Bender, R. Simms, S. Rodriguez-Eaton & C. Britt, 1995. Manganese removal from acid coal-mine drainage by a pond containing green algae and microbial mat. Water Science and Technology 31: 161–170.

    CAS  Google Scholar 

  64. Pohlman, J. W., T. M. Iliffe & L. A. Cifuentes, 1997. A stable isotope study of organic cycling and the ecology of an anchialine cave ecosystem. Marine Ecology Progress Series 155: 17–27.

    CAS  Article  Google Scholar 

  65. Quast, C., E. Pruesse, P. Yilmaz, J. Gerken, T. Schweer, P. Yarza, J. Peplies & F. O. Glöckner, 2013. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research 41: D590–D596.

    CAS  Article  PubMed  Google Scholar 

  66. R Development Core Team, 2008. R: A Language and Environment for Statistical Computing. R Development Core Team, Vienna.

  67. Sakihara, T. S., B. D. Dudley, R. A. MacKenzie & J. P. Beets, 2015. Endemic grazers control benthic microalgal growth in a eutrophic tropical brackish ecosystem. Marine Ecology Progress Series 519: 29–45.

    CAS  Article  Google Scholar 

  68. Sarbu, S. M., T. C. Kane & B. K. Kinkle, 1996. A chemoautotrophically based cave ecosystem. Science 272: 1953–1955.

    CAS  Article  PubMed  Google Scholar 

  69. Sayers, E. W., T. Barrett, D. A. Benson, S. H. Bryant, K. Canese, V. Chetvernin, D. M. Church, M. DiCuccio, R. Edgar, S. Federhen, M. Feolo, L. Y. Geer, W. Helmberg, Y. Kapustin, D. Landsman, D. J. Lipman, T. L. Madden, D. R. Maglott, V. Miller, I. Mizrachi, J. Ostell, K. D. Pruitt, G. D. Schuler, E. Sequeira, S. T. Sherry, M. Shumway, K. Sirotkin, A. Souvorov, G. Starchenko, T. A. Tatusova, L. Wagner, E. Yaschenko & J. Ye, 2009. Database resources of the National Center for Biotechnology Information. Nucleic Acids Research 37: D5–15.

    CAS  Article  PubMed  Google Scholar 

  70. Seidel, B., A. Brasher, K. Auerswald & J. Geist, 2016. Physicochemical characteristics, community assemblages, and food web structure in anchialine pools along the Kona Coast on the Island of Hawaii, USA. Hydrobiologia 770: 225–241.

    CAS  Article  Google Scholar 

  71. Seymour, J. R., W. F. Humphreys & J. G. Mitchell, 2007. Stratification of the microbial community inhabiting an anchialine sinkhole. Aquatic Microbial Ecology 50: 11–24.

    Article  Google Scholar 

  72. Shannon, C. E., 2001. A mathematical theory of communication. SIGMOBILE Mobile Computing and Communications Review 5: 3–55.

    Article  Google Scholar 

  73. Simpson, E. H., 1949. Measurement of diversity. Nature 163: 688.

    Article  Google Scholar 

  74. Sket, B., 1996. The ecology of anchihaline caves. Trends in Ecology & Evolution 11: 221–225.

    CAS  Article  Google Scholar 

  75. Stock, J. H., T. M. Iliffe & D. Williams, 1986. The concept ‘anchialine’reconsidered. Stygologia 2: 90–92.

    Google Scholar 

  76. Thomas, M. L. H., A. Logan, K. E. Eakins & S. M. Mathers, 1992. Biotic characteristics of the anchialine ponds of Bermuda. Bulletin of Marine Science 50: 133–157.

    Google Scholar 

  77. Walker, J. J., J. R. Spear & N. R. Pace, 2005. Geobiology of a microbial endolithic community in the Yellowstone geothermal environment. Nature 434: 1011–1014.

    CAS  Article  PubMed  Google Scholar 

  78. Walter, M. R., J. Bauld & T. D. Brock, 1972. Siliceous algal and bacterial stromatolites in hot spring and geyser effluents of Yellowstone National Park. Science 178: 402–405.

    CAS  Article  PubMed  Google Scholar 

  79. Wong, D. C. L., 1975. Algae of the anchialine pools at Cape Kinau, Maui, and aspects of the trophic ecology of Halocaridina rubra Holthuis (Decapoda, Atyidae). Master of Science Thesis, University of Hawaii.

  80. Wu, Q. L., G. Zwart, M. Schauer, M. P. K. Agterveld & M. W. Hahn, 2006. Bacterioplankton community composition along a salinity gradient of sixteen high-mountain lakes located on the Tibetan Plateau, China. Applied and Environmental Microbiology 72: 5478–5485.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  81. Yager, J., 1981. Remipedia, a new class of Crustacea from a marine cave in the Bahamas. Journal of Crustacean Biology 1: 328–333.

    Article  Google Scholar 

  82. Yang, J., L. Ma, H. Jiang, G. Wu, & H. Dong, 2016. Salinity shapes microbial diversity and community structure in surface sediments of the Qinghai-Tibetan Lakes. Scientific Reports 6. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844989/.

  83. Zepeda, V. K., H.-J. Busse, J. Golke, J. H. Saw, M. Alam & S. P. Donachie, 2015. Terasakiispira papahanaumokuakeensis gen. nov., sp. nov., a Gammaproteobacterium from Pearl and Hermes Atoll, Northwestern Hawaiian Islands. International journal of systematic and evolutionary microbiology 65: 3609–3617.

    Article  PubMed  Google Scholar 

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Acknowledgements

We thank K. L. Kim and R. A. Kinzie III for generous help and support associated with fieldwork. P. M. Brannock and D. S. Waits graciously assisted with processing of sequence data. We are indebted to P.M. Brannock and M. Newman for providing helpful comments and feedback during the writing process. M. Ramsey assisted and provided comments and photos, regarding work at the WC site. Site access and collections were conducted under the following scientific permits: State of Hawaii Native Invertebrate Research Permit # FHM10-232 and MAKA: Kamehameha Schools Permit # 4803. The experiments conducted in this study comply with current laws of the United States and the State of Hawaii. Funding support for this work came from the National Science Foundation (DEB #0949855 to S.R.S). This represents contributions No. 162 and No. 67 to the Auburn University (AU) Marine Biology Program and Molette Biology Laboratory for Environmental and Climate Change Studies, respectively.

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This study was funded by the National Science Foundation (DEB #0949855 to S.R.S).

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Fig. S1

Schematic of data generation showing processing of a microbial community samples from DNA extraction through sequencing of the Bacteria-specific V6 hypervariable region of the 16S-rDNA gene or the Eukarya-biased V9 hypervariable region of the 18S-rDNA gene from anchialine habitats surveyed in Summer 2010 on the islands of Oahu, Maui, and Hawaii (EPS 88 kb)

Fig. S2

Diversity estimates as number of observed OTUs, Chao1, Shannon diversity, and Inverse Simpson diversity for a the Bacteria-specific V6 hypervariable region of the 16S-rDNA gene b Eukarya-biased V9 hypervariable region of the 18S-rDNA gene. Samples are grouped by benthos and water column communities within sites from anchialine habitats surveyed in Summer 2010 on the islands of Oahu, Maui, and Hawaii (EPS 1688 kb)

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Hoffman, S.K., Seitz, K.W., Havird, J.C. et al. Diversity and the environmental drivers of spatial variation in Bacteria and micro-Eukarya communities from the Hawaiian anchialine ecosystem. Hydrobiologia 806, 265–282 (2018). https://doi.org/10.1007/s10750-017-3365-2

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Keywords

  • Illumina amplicon sequencing
  • V6 hypervariable region 16S rDNA gene
  • V9 hypervariable region 18S rDNA gene
  • Microbial diversity