, Volume 802, Issue 1, pp 97–113 | Cite as

Ecophysiological characterization and toxin profile of two strains of Cylindrospermopsis raciborskii isolated from a subtropical lagoon in Southern Brazil

  • Maria Cecília MiottoEmail author
  • Luiza Dy Fonseca Costa
  • Débora Monteiro Brentano
  • Camila Nader
  • Luana dos Santos Souza
  • Pablo Diego Gressler
  • Roselane Laudares-Silva
  • João Sarkis Yunes
  • José Bonomi Barufi
  • Leonardo Rubi Rörig
Primary Research Paper


In the present work, we attempted to characterize two isolates of Cylindrospermopsis raciborskii, LP1 and LP2, from Peri Lagoon, for their morphology, ecophysiology, and toxin profiles. The genetic identity of the isolates was confirmed by amplifying and sequencing 16S rRNA. The isolates showed different morphologies and significant differences in the length of trichomes. LP2 showed a trend for higher growth rates than LP1 at the different temperatures and N:P ratios. Both isolates showed low light requirements, but were able to tolerate irradiances of around 200 μmol photons m−2 s−1. LP2 showed higher concentrations of saxitoxin than LP1 and wider range of analogs, therefore being considered more toxic. These results support the hypothesis of ecotype selection for this species, which probably originated in response to environmental fluctuations in Peri Lagoon. Dominance during almost the entire year can be explained by the alternation of these ecotypes in the total biomass contribution according to their physiological advantages, contributing to the ecological success of this species.


Cyanobacteria Ecotypes Cyanotoxins Saxitoxin 



We would like to thank the Coordination for the Improvement of Higher Education Personnel (CAPES—Brazil) for providing the scholarship, National Council for Scientific and Technological Development (CNPq) and Fundação de Amparo à Pesquisa e Inovação de Santa Catarina (FAPESC) for research funding, Cecília Barberena de Vinatea for isolating LP1, Mateus Reck and Gustavo Klabunde for their help and support in molecular analyses and Eduardo de Oliveira Bastos for taking and editing the images.


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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • Maria Cecília Miotto
    • 1
    Email author
  • Luiza Dy Fonseca Costa
    • 2
  • Débora Monteiro Brentano
    • 3
  • Camila Nader
    • 1
  • Luana dos Santos Souza
    • 1
  • Pablo Diego Gressler
    • 1
  • Roselane Laudares-Silva
    • 1
  • João Sarkis Yunes
    • 2
  • José Bonomi Barufi
    • 1
  • Leonardo Rubi Rörig
    • 1
  1. 1.Laboratory of Phycology, Department of BotanyFederal University of Santa Catarina (UFSC)FlorianópolisBrazil
  2. 2.Laboratory of Cyanobacteria and Phycotoxins, Institute of OceanographyFederal University of Rio Grande (FURG)Rio GrandeBrazil
  3. 3.Laboratory of Ecotoxicology, Federal Institute of EducationScience and Technology of Santa Catarina (IFSC)FlorianópolisBrazil

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