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Sepiolid paralarval diversity in a regional upwelling area of the NE Atlantic

  • CEPHALOPOD ECOLOGY AND LIFE CYCLES
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Abstract

Sepiolid paralarvae are poorly studied, at least in part, because of the difficulty of accurate identification using morphological analysis. To unravel the biodiversity of sepiolid paralarvae collected in the Ría de Vigo during the upwelling season (2012–2014), and to overcome the difficulties of traditional identification, sepiolid paralarvae were identified by amplifying the barcoding gene cytochrome c oxidase subunit I (COI). In addition, morphometric analysis (Generalised Lineal Models, GLM and Discriminant Analysis, DA) was used to identify morphometric patterns useful for paralarval species identification. Genetic barcoding successfully identified 34 Sepiola pfefferi, 31 Rondeletiola minor, 30 Sepiola tridens, 4 Sepiola atlantica, 2 Sepietta neglecta and 1 Sepiola ligulata. COI analysis also allowed us to infer that the paralarvae of the three most abundant species belonged to the same populations independently of the year sampled. GLM suggested that total length (statistically different among the three species) and tentacle length (statistically larger in S. pfefferi from the other two species) were good variables to distinguish among species. DA succeeded in separating S. pfefferi from S. tridens, but R. minor overlapped along the first axes with both species, decreasing the accurate classification rate to 67%.

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Acknowledgements

Funding was provided by Ministerio de Economía y Competitividad (Grant Nos. CTM-2011- 25929 and BES – 2012-055651), Fundación Barrié (Grant No. Postdoctoral Grant), and La Trobe University (Grant No. RFWE).

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Authors and Affiliations

  1. Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello 6, 36208, Vigo, Spain

    Lorena Olmos-Pérez, Álvaro Roura & Ángel F. González

  2. La Trobe University, Bundoora, Melbourne, VIC, Australia

    Álvaro Roura

  3. Oceanlab, University of Aberdeen, Main Street, Newburgh, Aberdeenshire, AB41 6AA, UK

    Graham J. Pierce

  4. CESAM & Departamento de Biologia, Universidade de Aveiro, 3810-193, Aveiro, Portugal

    Graham J. Pierce

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  1. Lorena Olmos-Pérez
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  4. Ángel F. González
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Correspondence to Lorena Olmos-Pérez.

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Guest editors: Erica A. G. Vidal, Ian G. Gleadall & Natalie Moltschaniswskyi / Advances in Cephalopod Ecology and Life Cycles

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Supplementary Material 1

Measures undertaken in all sepiolid paralarvae: dorsal mantle length (DML), total length (TL), eye distance (ED) and tentacle length (TeL). Supplementary material 1 (TIFF 25684 kb)

Supplementary Material 2

Graphic representation of the values of the two discriminant equations and the group centroids. Supplementary material 2 (TIFF 34471 kb)

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Olmos-Pérez, L., Roura, Á., Pierce, G.J. et al. Sepiolid paralarval diversity in a regional upwelling area of the NE Atlantic. Hydrobiologia 808, 57–70 (2018). https://doi.org/10.1007/s10750-017-3186-3

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