, Volume 792, Issue 1, pp 151–168 | Cite as

Phylogeography and population genetics of the European mudminnow (Umbra krameri) with a time-calibrated phylogeny for the family Umbridae

  • Saša Marić
  • David Stanković
  • Josef Wanzenböck
  • Radek Šanda
  • Tibor Erős
  • Péter Takács
  • András Specziár
  • Nenad Sekulić
  • Doru Bănăduc
  • Marko Ćaleta
  • Ilya Trombitsky
  • László Galambos
  • Sándor Sipos
  • Aleš SnojEmail author
Primary Research Paper


The genetic structure of European mudminnow populations throughout the species range was examined using mitochondrial DNA and seven microsatellite loci. Ten mitochondrial haplotypes were detected, suggesting three phylogeographic lineages, which likely diverged during the Early and Middle Pleistocene. These three lineages geographically correspond to three regions: the Danube drainage including the Drava system and Dniester Delta, the Sava system and the Tisza system. High genetic diversity observed using mtDNA was confirmed with microsatellite data, suggesting the existence of 14 populations in the studied area. The isolation-with-migration model showed that migration rates between populations were generally low and were highest between the Drava and its tributary Mura. According to the inferred relative population splitting times, Umbra krameri likely spread from the eastern part of the species range to the west, which also showed the highest genetic diversity and largest population size. As reported by the time-calibrated phylogeny, separation of the European and American Umbra occurred roughly at the end of Late Cretaceous and in the first half of the Paleogene (60.57 Ma with 95% highest probability density of 39.57–81.75). Taking these results into account, appropriate guidelines are proposed to conserve European mudminnow populations.


Umbra krameri Umbridae mtDNA Microsatellites Time-calibrated phylogeny Conservation 



This study received support from the Slovenian Research Agency, the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 173045) and the SYNTHESYS Project (CZ-TAF-5090; financed by European Community Research Infrastructure Action under the FP7 Integrating Activities Programme. RŠ was supported by the Ministry of Culture of the Czech Republic (DKRVO 2016/15, National Museum, 00023272) and DS by the DIANET postdoctoral fellowship programme (FP1527385002). TE, PT and ASp would like to express their thanks for Eszter Csoma for the DNA extraction of the Hungarian samples. Many thanks to dr Ladislav Pekarik for collected samples of European mudminnow from the Slovak Republic.

Supplementary material

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Supplementary material 1 (DOC 82 kb)
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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Saša Marić
    • 1
  • David Stanković
    • 2
    • 3
  • Josef Wanzenböck
    • 4
  • Radek Šanda
    • 5
  • Tibor Erős
    • 6
  • Péter Takács
    • 6
  • András Specziár
    • 6
  • Nenad Sekulić
    • 7
  • Doru Bănăduc
    • 8
  • Marko Ćaleta
    • 9
  • Ilya Trombitsky
    • 10
  • László Galambos
    • 11
  • Sándor Sipos
    • 12
  • Aleš Snoj
    • 2
    Email author
  1. 1.Institute of Zoology, Faculty of BiologyUniversity of BelgradeBelgradeSerbia
  2. 2.Department of Animal Science, Biotechnical FacultyUniversity of LjubljanaDomžaleSlovenia
  3. 3.Department of Life SciencesUniversity of TriesteTriesteItaly
  4. 4.Research Institute for LimnologyUniversity of InnsbruckMondseeAustria
  5. 5.Department of ZoologyNational MuseumPrague 1Czech Republic
  6. 6.MTA Centre for Ecological ResearchBalaton Limnological InstituteTihanyHungary
  7. 7.Institute for Nature Conservation of SerbiaNovi BeogradSerbia
  8. 8.Faculty of SciencesLucian Blaga University of SibiuSibiuRomania
  9. 9.Faculty of Teacher EducationUniversity of ZagrebZagrebCroatia
  10. 10.Eco-TIRAS International Association of Dniester River KeepersChisinauMoldova
  11. 11.Institute for Nature Conservation of VojvodinaNovi SadSerbia
  12. 12.Department of Biology and Ecology, Faculty of ScienceUniversity of Novi SadNovi SadSerbia

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