, Volume 810, Issue 1, pp 133–144 | Cite as

An improved method for in vitro culture of glochidia in freshwater mussel Cristaria plicata (Mollusca, Bivalvia)

  • Xue-Yan Ma
  • Hai-Bo Wen
  • Jun Zou
  • Wu Jin
  • Dan Hua
  • Ruo-Bo GuEmail author
  • Pao XuEmail author


We established an improved method for in vitro culture of glochidia in freshwater mussel Cristaria plicata and compared the transformation percentage in L-15 basic medium supplemented with different plasma from host fish or two non-host fish species. The results showed that the metamorphosis rate of glochidia cultured with host fish plasma and non-host fish plasma was all above 96%. High-density lipoprotein and low-density lipoprotein (LDL) levels were significantly lower in host fish plasma than in non-host fish plasma (P < 0.05), suggesting that LDL may be an important nutrient for glochidia metamorphosis. There were no significant differences in the 15-day survival and growth of juvenile mussels obtained from groups with non-host fish plasma under 1100/dish (C5) and a high concentration of antibiotics (D7), as compared with the control (A4) (P > 0.05). The method developed herein not only provides a simplified in vitro culture method for glochidia at a reduced cost, but it also improves the culture efficiency. The method and medium composition may be further improved in studies investigating the long-term growth of juvenile mussels, and it would greatly benefit artificial breeding and germplasm resource preservation for this important species.


Non-parasitic metamorphosis Metamorphosis rate Nutrition factor Juvenile mussel culture 



The authors would like to thank Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2016GH10), National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center (2015JBFM02) and Ocean and Fishery Bureau of Jiangsu Province (Y2014-38), for providing financial support for this project. We also appreciate the insightful comments from the anonymous reviewers.


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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  1. 1.Wuxi Fishery CollegeNanjing Agriculture UniversityWuxiChina
  2. 2.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiChina
  3. 3.Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiChina

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