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Hydrobiologia

, Volume 784, Issue 1, pp 349–365 | Cite as

Genetic characterization of the Asian clam species complex (Corbicula) invasion in the Iberian Peninsula

  • Luis Peñarrubia
  • Rosa-Maria Araguas
  • Oriol Vidal
  • Carles Pla
  • Jordi Viñas
  • Nuria SanzEmail author
Primary Research Paper

Abstract

The Asian clam (Corbicula sp.) is an invasive freshwater bivalve native to Asia, the Middle East, Australia, and Africa. It is now widely distributed around the world producing large ecological and economic impacts. Three well-described invasive lineages form a cryptic species complex with asexual reproduction based on androgenesis. In this study, we collected 175 individuals from different Iberian, European, and North American locations to genetically study Corbicula invasion in the Iberian Peninsula using COI and 28S genes. The use of mitochondrial and nuclear markers allows us to characterize both maternal and paternal inheritance from androgenetic Corbicula locations and to deal with the incongruences caused by egg parasitism. We identified 7 COI and 10 28S haplotypes that grouped individuals within the three invasive Corbicula lineages. Haplotype distribution of mitochondrial and nuclear markers detected genetic divergence between the Ebro Delta location and the rest of Iberian sites, suggesting that at least two invasion episodes occurred in the Iberian Peninsula. Haplotype distribution also suggested secondary contacts between Iberian and other European invaded regions. Additionally, results revealed that nuclear hybridization, a feature more widespread than previously reported, contributes to retain gene diversity in the Corbicula invasion.

Keywords

Corbicula Iberian Peninsula Invasive species Mitochondrial COI Nuclear 28S Population structure 

Notes

Acknowledgments

We are indebted to different colleagues for Corbicula sample collections: J.C Pérez-Quintero (Universidad de Huelva), R. Araujo (CSIC), R. Miranda (Universidad de Navarra), J. Checa (Equipos y Suministros, S.L.), C. Alcaraz (IRTA, Spain), I. Planas and R. Puig (Nyctibios Association), J. F. Fruget (Université de Lyon), O. Popa and A. M. Krapaland (National Museum of Natural History, Romania), C. Robertson (Texas Parks and Wildlife Department, TX, USA) and J. Alvarado-Bremer (A&M University at Galveston, TX, USA). This research was carried out within the objectives of the research project CGL200909407 of the Spanish Ministerio de Ciencia e Innovación (MICINN). LP received a PhD fellowship support of the Spanish MICINN with reference BES—2010037446.

Supplementary material

10750_2016_2888_MOESM1_ESM.docx (75 kb)
Supplementary material 1 (DOCX 75 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Laboratori d’Ictiologia Genètica, Departament de BiologiaUniversitat de GironaGironaSpain

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