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Hydrobiologia

, Volume 789, Issue 1, pp 91–106 | Cite as

Acclimation to hypercarbia protects cardiac contractility and alters tissue carbohydrate metabolism in the Amazonian armored catfish Pterygoplichthys pardalis

  • T. J. MacCormackEmail author
  • J. L. Robinson
  • V. M. F. Almeida-Val
  • A. L. Val
  • W. R. Driedzic
ADAPTA

Abstract

The armored catfish Pterygoplichthys pardalis tolerates environmental hypercarbia, high partial pressures of CO2 (\(P_{{{\text{CO}}_{ 2} }}\)), by preferentially protecting intracellular pH (pHi) in the face of extracellular acidosis. This response is associated with ionic changes which may disrupt contractility in cardiac muscle, and it is not known whether acclimation to hypercarbia provides protection against these changes. We studied the influence of different \(P_{{{\text{CO}}_{ 2} }}\) acclimation histories on cardiac muscle function using isometrically contracting ventricular strip preparations. Fish were held for >4 months at 21 mmHg \(P_{{{\text{CO}}_{ 2} }}\) and then exposed to normocarbia (6 mmHg \(P_{{{\text{CO}}_{ 2} }}\)) for either 15 h or 5–6 days. Acclimation to chronic hypercarbia eliminated the negative inotropic effects of in vitro hypercarbia, decreased extracellular Ca2+ sensitivity, and reduced maximum pacing frequency in ventricular strip preparations. Fish acclimated to chronic hypercarbia also exhibited hepatic glycogen and plasma glucose accumulation, and lower plasma lactate levels compared to fish acclimated to normocarbia for 5–6 days. We suggest chronic hypercarbia may induce cardiac remodeling to protect contractility and reduce the energetic demands of pHi regulation. The activation of HCO3 synthesis pathways may decrease glucose utilization and enhance carbohydrate stores, potentially providing protection against hypoxia, a stressor frequently encountered in conjunction with hypercarbia in the Amazon.

Keywords

Acid–base regulation Hypercapnia pHe pHi Intracellular pH regulation Fish heart 

Notes

Acknowledgements

TJM and WRD were supported by Natural Sciences and Engineering Research Council of Canada Discovery grants. WRD holds the Canada Research Chair in Marine Bioscience. JLR was supported by a travel grant from Memorial University of Newfoundland. The experiments were supported by INCT ADAPTA (CNPq/FAPEAM) and by the Brazilian National Institute for Research in the Amazon (INPA) grants to ALV. ALV holds a research fellowship from the Brazilian National Counsel of Technological and Scientific Development (CNPq). The authors thank Nazare De Paula Silva and the staff at LEEM for technical assistance, Connie Short for biochemical analyses, and Maria Thistle for statistical advice.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • T. J. MacCormack
    • 1
    Email author
  • J. L. Robinson
    • 2
  • V. M. F. Almeida-Val
    • 3
  • A. L. Val
    • 3
  • W. R. Driedzic
    • 4
  1. 1.Department of Chemistry and BiochemistryMount Allison UniversitySackvilleCanada
  2. 2.Department of BiochemistryMemorial University of NewfoundlandSt. John’sCanada
  3. 3.Laboratory of Ecophysiology and Molecular EvolutionInstituto Nacional Pesquisa da AmazôniaManausBrazil
  4. 4.Department of Ocean SciencesMemorial University of NewfoundlandSt. John’sCanada

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