Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress
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Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city’s main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.
KeywordsUnio pictorum Unio tumidus Comet assay
This research received funding from the European Community’s FP7/2007-2013 Agreement No. 265264.
- Ahmed, M. K., M. Habibullah-Al-Mamun, M. A. Hossain, M. Arif, E. Parvin, M. S. Akter, M. S. Khan & M. M. Islam, 2011. Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test. Chemosphere 84: 143–149.PubMedCrossRefGoogle Scholar
- Ali, D., S. Alarifi, S. Kumar, M. Ahamed & M. A. Siddiqui, 2012. Oxidative stress and genotoxic effect of zinc oxide nanoparticles in freshwater snail Lymnaea luteola L. Aquatic Toxicology 15: 124–125.Google Scholar
- Bagdonas, E. & M. Z. Vosyliene, 2006. A study of toxicity and genotoxicity of copper, zinc and their mixture to rainbow trout (Oncorhynchus Mykiss). Biologija 1: 8–13.Google Scholar
- Bauer, G. & K. Wachtler, 2001. Ecology and evolution of the freshwater mussels unionoida. In Bauer, G. & K. Wachtler. (eds), Ecological Studies. Vol. 145. Berlin: Springer.Google Scholar
- Bolognesi, C., A. Buschini, E. Branchi, P. Carboni, M. Furlini, A. Martino, M. Monteverde, P. Poli & C. Rossi, 2004. Comet and micronucleus assays in zebra mussel cells for genotoxicity assessment of surface drinking water treated with three different disinfectants. Science of the Total Environment 333: 127–136.PubMedCrossRefGoogle Scholar
- Collins, A. R., A. Oscoz, G. Brunborg, I. Gaiva, L. Giovannelli, M. Kruszewski, C. C. Smith & R. Stetina, 2008. The comet assay: topical issues. Mutagenesis 23: 143–151.Google Scholar
- Conners, E. D. & C. M. Black, 2004. Evaluation of lethality and genotoxicity in the freshwater mussel Utterbackia imbecillis (Bivalvia: Unionidae) exposed singly and in combination to chemicals used in Lawn Care. Archives of Environmental Contamination and Toxicology 46: 362–371.PubMedCrossRefGoogle Scholar
- De Kock, W. C. & K. J. M. Kramer, 1994. Active biomonitoring (ABM) by translocation of Bivalves mollusks. In Kramer, K. J. M. (ed.), Biomonitoring of Coastal Waters and Estuaries. CRC Press, Boca Raton, FL: 51–84.Google Scholar
- European Commission, 2006. Directive 2006/7/EEC, Directive concerning management of bathing water quality and repealing Directive 76/160/EEC. Official Journal of European Communities L. 64: 37–51.Google Scholar
- Guidi, P., G. Frenzilli, M. Benedetti, M. Bernardeschi, A. Falleni, D. Fattorini, F. Regoli, V. Scarcelli & M. Nigro, 2010. Antioxidant, genotoxic and lysosomal biomarkers in the freshwater bivalve (Unio pictorum) transplanted in a metal polluted river basin. Aquatic Toxicology 100: 75–83.PubMedCrossRefGoogle Scholar
- ICPDR, 2002. Water Quality in the Danube River Basin. TNMN Yearbook 2002. http://www.icpdr.org/icpdr-pages/tnmn_yearbooks.htm.
- Lehtonen, K. K., D. Schiedek, A. Köhler, T. Lang, P. J. Vuorinen, L. Förlin, J. Barŝiené, J. Pempkowiak & J. Gercken, 2006. The BEEP project in the Baltic Sea: overview of results and outline for a regional biological effects monitoring strategy. Marine Pollution Bulletin 53: 523–537.PubMedCrossRefGoogle Scholar
- Olive, P. L. & P. B. Judit, 2006. The comet assay: a method to measure DNA damage in individual cells. British Columbia Cancer Research Center. doi: 10.1038/nprot.2006.5675.
- Ravera, O., 2001. Monitoring of the aquatic environment by species accumulators of pollutants: a review, in: Ravera, O. (eds), Scientific and legal aspects of biological monitoring in freshwater. Journal of Limnology 60: 63–72.Google Scholar
- Regoli, F., G. Frenzilli, R. Bocchetti, F. Annarumma, V. Scarcelli, D. Fattorini & M. Nigro, 2004. Time-course variations of oxyradical metabolism, DNA integrity and lysosomal stability in mussels, Mytilus galloprovincialis during a field translocation experiment. Aquatic toxicology 68: 167–178.PubMedCrossRefGoogle Scholar
- Soares-da-Silva, I. M., J. Ribeiro, C. Valongo, R. Pinto, M. Vilanova, R. Bleher & J. Machado, 2002. Cytometric, morphologic and enzymatic characterization of haemocytes in Anodonta cygnea. Comp. Biochem Physiol. Part A: Mol. Integr. Physiol. 132: 541–553.Google Scholar
- StatSoft, Inc., 2001. STATISTICA for Windows [Computer program manual]. Tulsa, OK: StatSoft, Inc., http://www.statsoft.com.
- Sunjog, K., Z. Gačić, S. Kolarević, Z. Višnjić-Jeftić, I. Jarić, J. Knežević-Vukčević, B. Vuković-Gačić, & M. Lenhardt, 2012. Heavy metal accumulation and the genotoxicity in Barbel (Barbus barbus) as indicators of the Danube river pollution. The Scientific World Journal. doi: 10.1100/2012/351074.
- Viarengo, A., D. Lowe, C. Bolognesi, E. Fabbri & A. Koehler, 2007. The use of biomarkers in biomonitoring: a 2-tier approach assessing the level of pollutant-induced stress syndrome in sentinel organisms. Comparative biochemistry and physiology. Toxicology & Pharmacology 146: 281–300.Google Scholar