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Hydrobiologia

, Volume 715, Issue 1, pp 63–76 | Cite as

Food quality of mixed bacteria–algae diets for Daphnia magna

  • Heike M. FreeseEmail author
  • Dominik Martin-Creuzburg
CLADOCERA

Abstract

Bacteria can comprise a large fraction of seston in aquatic ecosystems and can therefore significantly contribute to diets of filter-feeding zooplankton. To assess the effect of three heterotrophic bacteria (Flavobacterium sp., Pseudomonas sp. and Escherichia coli) on survival, growth and egg production of juvenile Daphnia magna during six-day growth experiments, five ratios of bacteria–Scenedesmus obliquus mixtures were fed. Potential growth-limiting effects mediated by essential biochemicals were assessed upon supplementation of pure bacterial diets with a sterol (cholesterol) or a polyunsaturated fatty acid (EPA). Pure bacterial diets always had detrimental effects on Daphnia. However, cholesterol supplementation of Flavobacterium sp. enhanced growth rates of Daphnia. Diets containing Pseudomonas impaired Daphnia growth even at low dietary proportions (20%), indicating their toxicity. In contrast, Daphnia grew at relative high dietary proportions of Flavobacterium sp. and E. coli (80–50%). In fact, diets containing small proportions of these heterotrophic bacteria (Flavobacterium ≤50%, E. coli 20%) even significantly increased Daphnia growth rates compared to pure algal diets, indicating a nutritional upgrading by these bacteria. Our results suggest that the relative contribution of bacteria and phytoplankton to total dietary carbon as well as their phylogenetic composition strongly influence Daphnia fitness and potentially other filter-feeding zooplankton under field conditions.

Keywords

Flavobacterium sp. Pseudomonas sp. Escherichia coli Polyunsaturated fatty acids Sterols Scenedesmus 

Notes

Acknowledgment

We thank A. Wiese for technical assistance.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Department of Biology, Microbial EcologyUniversity of KonstanzKonstanzGermany
  2. 2.Microbial Ecology and Diversity ResearchLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und ZellkulturenBraunschweigGermany
  3. 3.Limnological InstituteUniversity of KonstanzKonstanzGermany

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