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Taxonomic and phylogenetic evaluation of Limnothrix strains (Oscillatoriales, Cyanobacteria) by adding Limnothrix planktonica strains isolated from central China

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Abstract

Six Limnothrix strains, isolated for the first time from a shallow eutrophic lake in central China, were taxonomically and phylogenetically evaluated by investigating their polyphasic characteristics, including morphological features, cellular ultrastructures, and 16S rRNA gene sequences. All the six strains were morphologically similar, and their trichomes were in average 1.7 μm wide and cells 4.0 μm long, and having small gas vesicles within cells, and therefore identified as Limnothrix planctonica (Woloszynska) Meffert. Cellular ultrastructures of them showed that peripheral thylakoids with 3–5 parallel layers were parietally distributed in the cells. The phylogenetic results based on the 16S rRNA gene sequences showed that all the Limnothrix strains, including the six in this study and those from the Genbank, formed two distinct clusters. The similarity in 16S rDNA sequences between these two clusters was lower than 90%, indicating that these Limnothrix strains belong to different genera. This is the first report on the morphology and phylogeny of L. planctonica strains, providing the new information on taxonomy of the genus Limnothrix.

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Acknowledgments

This research was supported by the China National Natural Science Foundation (NSFC) (No. 30970185), the State Key Basic Research and Development Plan of China (2008CB418002) and the Research fund from Hubei Province Environmental Agency (Y03A061603).

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Authors and Affiliations

  1. Key Laboratory of Aquatic Biodiversity and Conservation Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, Hubei, People’s Republic of China

    Mengling Zhu, Gongliang Yu, Xiaochuang Li, Wenhua Tan & Renhui Li

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Mengling Zhu, Xiaochuang Li & Wenhua Tan

Authors
  1. Mengling Zhu
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  2. Gongliang Yu
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  3. Xiaochuang Li
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  4. Wenhua Tan
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  5. Renhui Li
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Correspondence to Renhui Li.

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Guest editors: N. Salmaso, L. Naselli-Flores, L. Cerasino, G. Flaim, M. Tolotti & J. Padisák / Phytoplankton responses to human impacts at different scales: 16th workshop of the International Association of Phytoplankton Taxonomy and Ecology (IAP)

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Zhu, M., Yu, G., Li, X. et al. Taxonomic and phylogenetic evaluation of Limnothrix strains (Oscillatoriales, Cyanobacteria) by adding Limnothrix planktonica strains isolated from central China. Hydrobiologia 698, 367–374 (2012). https://doi.org/10.1007/s10750-012-1127-8

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  • DOI: https://doi.org/10.1007/s10750-012-1127-8

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