Molecular phylogeny of glass sponges (Porifera, Hexactinellida): increased taxon sampling and inclusion of the mitochondrial protein-coding gene, cytochrome oxidase subunit I
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Marine sponges of the class Hexactinellida (glass sponges) are among the most understudied groups of Porifera, and molecular approaches to investigating their evolution have only recently emerged. Although these first results appeared reliable as they largely corroborated morphology-based hypotheses, they were almost exclusively based on ribosomal RNA genes (rDNA) and should, therefore, be further tested with independent types of genetic data, such as protein-coding genes. To this end, we established the mitochondrial-encoded cytochrome oxidase subunit I gene (COI) as an additional marker, and conducted phylogenetic analyses on DNA- and amino-acid level, as well as a supermatrix analysis based on combined COI DNA and rDNA alignments. Furthermore, we increased taxon sampling compared to previous studies by adding seven additional species. The COI-based phylogenies were largely congruent with the rDNA-based phylogeny but suffered from poor bootstrap support for many nodes. However, addition of the COI sequences to the rDNA data set increased resolution of the overall molecular phylogeny. Thus, although obtaining COI sequences from glass sponges turned out to be quite challenging, this gene appears to be a valuable supplement to rDNA data for molecular evolutionary studies of this group. Some implications of our extended phylogeny for the evolution and systematics of Hexactinellida are discussed.
KeywordsPorifera Hexactinellida Phylogeny Cytochrome oxidase subunit I Ribosomal DNA
Work at the Wörheide lab was supported by the Deutsche Forschungsgemeinschaft (DFG; project Wo896/5-3). The DFG is further acknowledged for funding the Deep Down Under expedition (Project Wo896/7-1; www.deepdownunder.de). During writing of this manuscript, MD was supported by a Postdoctoral Fellowship of the Smithsonian Institution. Work at the Lavrov lab was supported by the National Science Foundation (NSF) through the Poriferan Tree of Life project (DEB-0828783; https://www.portol.org/). Shirley Pomponi (HBOI), Sally Leys, and Jean Vacelet are acknowledged for providing additional specimens. Our warmest thanks go to the organizers of the 8th World Sponge Conference for making this great meeting possible. Allen Collins and three anonymous reviewers provided comments that led to improvement of this manuscript. Finally, we would like to thank Alexandros Stamatakis for implementing paired-sites models in his amazing program, RAxML.
- Dohrmann, M., O. Voigt, D. Erpenbeck & G. Wörheide, 2006. Non-monophyly of most supraspecific taxa of calcareous sponges (Porifera, Calcarea) revealed by increased taxon sampling and partitioned Bayesian analysis of ribosomal DNA. Molecular Phylogenetics and Evolution 40: 830–843.PubMedCrossRefGoogle Scholar
- Dohrmann, M., C. Göcke, D. Janussen, J. Reitner, C. Lüter & G. Wörheide, 2011. Systematics and spicule evolution in dictyonal sponges (Hexactinellida: Sceptrulophora) with description of two new species. Zoological Journal of the Linnean Society (in press).Google Scholar
- Erpenbeck, D. & G. Wörheide, 2007. On the molecular phylogeny of sponges (Porifera). Zootaxa 1668: 107–126.Google Scholar
- Göcke, C. & D. Janussen, 2011. ANT XXIV/2 (SYSTCO) Hexactinellida (Porifera) and bathymetric traits of Antarctic glass sponges (incorporating ANDEEP-material); including an emendation of the rediscovered genus Lonchiphora. Deep-Sea Research Part II: in press.Google Scholar
- Hillis, D. M. & J. J. Bull, 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42: 182–192.Google Scholar
- Mehl, D., 1992. Die Entwicklung der Hexactinellida seit dem Mesozoikum. Paläobiologie, Phylogenie und Evolutionsökologie. Berliner Geowissenschaftliche Abhandlungen Reihe E: Paläobiologie 2: 1–164.Google Scholar
- Philippe, H., R. Derelle, P. Lopez, K. Pick, C. Borchiellini, N. Boury-Esnault, J. Vacelet, E. Renard, E. Houliston, E. Quéinnec, C. Da Silva, P. Wincker, H. Le Guyader, S. Leys, D. J. Jackson, F. Schreiber, D. Erpenbeck, B. Morgenstern, G. Wörheide & M. Manuel, 2009. Phylogenomics revives traditional views on deep animal relationships. Current Biology 19: 706–712.PubMedCrossRefGoogle Scholar
- Pick, K. S., H. Philippe, F. Schreiber, D. Erpenbeck, D. J. Jackson, P. Wrede, M. Wiens, A. Alié, B. Morgenstern, M. Manuel & G. Wörheide, 2010. Improved phylogenomic taxon sampling noticeably affects non-bilaterian relationships. Molecular Biology and Evolution 27: 1983–1987.PubMedCrossRefGoogle Scholar